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公开(公告)号:US20210285936A1
公开(公告)日:2021-09-16
申请号:US16624062
申请日:2018-06-25
发明人: Robert Beech , Mikel Larson , Jacob A Bertrand , Gavi Begtrup
IPC分类号: G01N33/53 , G01N33/543 , G01N27/327
摘要: Electrochemical aptamer-based biosensing devices and methods are described herein that are configured to produce a detectible signal upon target analyte interaction, with reduced reliance on a conformational change by the aptamer. The disclosure includes embodiments of docked aptamer EAB sensors for measuring the presence of a target analyte in a biofluid sample. The sensors include an electrode capable of sensing redox events, and a plurality of aptamer sensing elements with aptamers selected to interact with a target analyte. Each aptamer sensing element includes a molecular docking structure attached to the electrode, and an analyte capture complex that includes an aptamer releasably bound to the docking structure, and an electroactive redox moiety. Upon the aptamer binding with a target analyte, the analyte capture complex separates from the docking structure. The separation of the analyte capture complex from the docking structure produces a positional change in the redox moiety that is detectable by the sensing device on interrogation of the electrode.
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公开(公告)号:US20210282671A1
公开(公告)日:2021-09-16
申请号:US16605979
申请日:2019-03-22
摘要: Embodiments of the disclosed invention provide wearable devices that use a humidity sensor to measure sweat rate generated from an area of skin. A sensing chamber is continuously filled with a sweat sample, which forms a droplet and alters the humidity measured within the chamber. Once the sweat sample droplet expands to the edge of the chamber, the droplet contacts a wick and is drawn away, so the chamber can fill with a subsequent droplet. The device uses a droplet volume and the time required to reach a maximum humidity to calculate a sweat rate. A pump is used to draw old sweat sample out of the wick to allow extended device operation. Some embodiments also include capacitive sensors to perform back up measurements. Another set of embodiments includes alternatively shaped sensing chambers configured to reduce sample volumes or improve function. A method for determining sweat rate based on humidity sensor measurements is also included.
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公开(公告)号:US20210204840A1
公开(公告)日:2021-07-08
申请号:US16087342
申请日:2017-03-21
IPC分类号: A61B5/1477 , G01N33/543 , A61B5/145 , A61B5/01 , A61B5/0533 , A61B5/00
摘要: Challenges of the sweat medium for electrochemical aptamer-based biosensor (“EAB sensor”) devices can be mitigated through aptamer selection, sensor/device configuration and physiological algorithms that account for the effects of (1) target analyte size, (2) potential concentration ranges, (3) sweat sample pH, and (4) sweat sample salinity. The disclosed invention includes a method of aptamer selection for use in an EAB sensor configured for use in a wearable sweat sensing device. The disclosed invention further provides a sweat sensing device configured to use EAB sensors to detect target analytes in a sweat sample.
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公开(公告)号:US10674946B2
公开(公告)日:2020-06-09
申请号:US15382703
申请日:2016-12-18
发明人: Gavi Begtrup , Jason Heikenfeld
摘要: The disclosed invention provides a sweat sensing device configured with self-aligned sweat stimulation means to provide adequate sweat generation for continuous monitoring of sweat. The disclosed device includes one or more analyte-specific sweat sensors that self-align with sweat glands. In one embodiment, the sweat sensing device includes means to protect the self-aligning sensors from abrasion against the skin or device components. In another embodiment, the device includes prolonged sweat stimulation for the self-aligning sensors through diffusion of a sweat stimulating compound into the skin.
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公开(公告)号:US10506968B2
公开(公告)日:2019-12-17
申请号:US15966476
申请日:2018-04-30
IPC分类号: A61B5/145 , A61B5/00 , G01N33/50 , A61B10/00 , A61B5/1468 , A61F13/02 , G01N33/66 , G01N33/53 , G01N33/543
摘要: The disclosed invention provides a fluid sensing device and method capable of collecting a fluid sample, concentrating the sample with respect to one or more target analytes, and measuring the target analyte(s) in the concentrated sample. The invention is also capable of determining the change in molarity of the fluid sample with respect to the target analyte(s), as the sample is concentrated by the device. The invention further includes a method for using a fluid sensing device to concentrate a fluid sample with respect to one or more target analytes. The disclosed method further includes the ability to correlate the measured target analyte concentration to a physiological condition of a device wearer, or of a fluid source.
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公开(公告)号:US20190117132A1
公开(公告)日:2019-04-25
申请号:US16083382
申请日:2017-03-09
发明人: Robert Beech , Gavi Begtrup , Benjamin Katchman
IPC分类号: A61B5/1477 , A61B5/00 , A61B5/145 , A61B5/117
摘要: The disclosed invention addresses an unmet need of biofluid analysis by utilizing the presence of certain nucleotides, e.g., microDNA, eccDNA, DNA fragments, microRNA, RNA fragments, and peptides, in a sample biofluid, such as eccrine sweat, apocrine sweat, blood, serum, saliva or tears, to perform a number of physiological sensing functions. Specifically, the present disclosure provides: (1) a device and method of determining whether a biofluid sensing device is being worn by a specific individual based on a measurement of nucleotides in a biofluid; (2) a device and method of recognizing at least one physiological disease or condition based on nucleotide concentrations, ratios, or trends in a biofluid.
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公开(公告)号:US20200080987A1
公开(公告)日:2020-03-12
申请号:US16344871
申请日:2017-10-25
IPC分类号: G01N33/487 , G16H50/30
摘要: The present disclosure provides a method of using a wearable biofluid sensing device to develop a cytokine profile for an individual. The method includes taking concentration, ratio, and trend measurements of one or more cytokines in the individual's sweat, along with other contemporaneous device measurements to inform sweat rate, skin temperature, sweat sample pH, or other factors. The method further considers these measured values in the context of external information about the individual, and uses such information to develop (1) a baseline cytokine profile characterizing the individual's healthy cytokine levels, or (2) an inflammation profile for a physiological condition, which characterizes the expected cytokine levels for a physiological condition. Also included is a method to use a biofluid sensing device to determine whether an individual has a physiological condition by comparing device measurements to the baseline profile and inflammation profile. Results are then communicated to a device user.
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公开(公告)号:US20190254641A1
公开(公告)日:2019-08-22
申请号:US16403094
申请日:2019-05-03
发明人: Gavi Begtrup , Jason Heikenfeld , Austin Morgan
摘要: The disclosed invention provides a biofluid sensing device configured with a membrane-enhanced sensor located in a sweat collector. The disclosed analyte-specific sensor is configured to reduce required biofluid sample volume due to its close proximity to the skin and source of sweat biofluid. The sensor is contained within a pH and salinity-stabilized fluid that, in turn, is contained in a selectively permeable membrane to improve sensor performance in variable biofluids, and to protect the sensor from skin contact. In one embodiment, the biofluid sensing device includes means to protect the self-aligning sensors from abrasion against the skin or device components. Other embodiments of the disclosed invention include a track-etched membrane to provide sensor protection.
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公开(公告)号:US20190142311A1
公开(公告)日:2019-05-16
申请号:US16163011
申请日:2018-10-17
IPC分类号: A61B5/145 , A61B5/1495 , A61B5/00
摘要: Devices for calibrating electrochemical aptamer-based biofluid sensors. In some embodiments a calibrant is located proximate to the EAB sensors, and calibration is facilitated by introduction of the biofluid into the device. Other embodiments include various mechanisms for introducing calibration solution to the sensors, including drawing calibration solution into contact with sensors through electrical potential, a dispensing mechanism such as an ink jet-type nozzle, as well as pressure actuated calibration dispensing. Also included are embodiments employing bifurcated biofluid transport paths that allow alternately for calibration and biofluid sensing.
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公开(公告)号:US20190142309A1
公开(公告)日:2019-05-16
申请号:US16206095
申请日:2018-11-30
IPC分类号: A61B5/1477 , A61B5/00 , A61B5/145
摘要: The disclosed invention provides a biofluid collection device configured with an open microfluidic network, which facilitates nanoliter-scale biofluid collection and transport for biosensing applications. In one embodiment, a biofluid sensing device placed on the skin for measuring a characteristic of an analyte in sweat includes one or more biofluid sensors and a hexagonal open microfluidic network biofluid collector. The disclosed collector provides a volume-reduced pathway for sweat biofluid between the one or more sensors and sweat glands when the device is positioned on the skin. In another embodiment, a biofluid collector includes a network of microchannels comprising three or more repeatedly intersecting channels that provide redundant pathways for biofluid transport. Embodiments of the disclosed invention are also directed to highly stable peptide-based self-assembled monolayers (SAM) and methods of making the SAMs. In some embodiments, the peptide-based SAM is formed on a component of a biofluid sensing device.
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