DOCKED APTAMER EAB BIOSENSORS
    1.
    发明申请

    公开(公告)号:US20210285936A1

    公开(公告)日:2021-09-16

    申请号:US16624062

    申请日:2018-06-25

    摘要: Electrochemical aptamer-based biosensing devices and methods are described herein that are configured to produce a detectible signal upon target analyte interaction, with reduced reliance on a conformational change by the aptamer. The disclosure includes embodiments of docked aptamer EAB sensors for measuring the presence of a target analyte in a biofluid sample. The sensors include an electrode capable of sensing redox events, and a plurality of aptamer sensing elements with aptamers selected to interact with a target analyte. Each aptamer sensing element includes a molecular docking structure attached to the electrode, and an analyte capture complex that includes an aptamer releasably bound to the docking structure, and an electroactive redox moiety. Upon the aptamer binding with a target analyte, the analyte capture complex separates from the docking structure. The separation of the analyte capture complex from the docking structure produces a positional change in the redox moiety that is detectable by the sensing device on interrogation of the electrode.

    HUMIDITY-BASED SWEAT RATE SENSING DEVICES

    公开(公告)号:US20210282671A1

    公开(公告)日:2021-09-16

    申请号:US16605979

    申请日:2019-03-22

    IPC分类号: A61B5/145 A61B10/00 A61B5/01

    摘要: Embodiments of the disclosed invention provide wearable devices that use a humidity sensor to measure sweat rate generated from an area of skin. A sensing chamber is continuously filled with a sweat sample, which forms a droplet and alters the humidity measured within the chamber. Once the sweat sample droplet expands to the edge of the chamber, the droplet contacts a wick and is drawn away, so the chamber can fill with a subsequent droplet. The device uses a droplet volume and the time required to reach a maximum humidity to calculate a sweat rate. A pump is used to draw old sweat sample out of the wick to allow extended device operation. Some embodiments also include capacitive sensors to perform back up measurements. Another set of embodiments includes alternatively shaped sensing chambers configured to reduce sample volumes or improve function. A method for determining sweat rate based on humidity sensor measurements is also included.

    Sweat sensing devices with sensor abrasion protection

    公开(公告)号:US10674946B2

    公开(公告)日:2020-06-09

    申请号:US15382703

    申请日:2016-12-18

    IPC分类号: A61B5/145 A61N1/04 A61B5/00

    摘要: The disclosed invention provides a sweat sensing device configured with self-aligned sweat stimulation means to provide adequate sweat generation for continuous monitoring of sweat. The disclosed device includes one or more analyte-specific sweat sensors that self-align with sweat glands. In one embodiment, the sweat sensing device includes means to protect the self-aligning sensors from abrasion against the skin or device components. In another embodiment, the device includes prolonged sweat stimulation for the self-aligning sensors through diffusion of a sweat stimulating compound into the skin.

    BIOFLUID SENSING DEVICE NUCLEOTIDE SENSING APPLICATIONS

    公开(公告)号:US20190117132A1

    公开(公告)日:2019-04-25

    申请号:US16083382

    申请日:2017-03-09

    摘要: The disclosed invention addresses an unmet need of biofluid analysis by utilizing the presence of certain nucleotides, e.g., microDNA, eccDNA, DNA fragments, microRNA, RNA fragments, and peptides, in a sample biofluid, such as eccrine sweat, apocrine sweat, blood, serum, saliva or tears, to perform a number of physiological sensing functions. Specifically, the present disclosure provides: (1) a device and method of determining whether a biofluid sensing device is being worn by a specific individual based on a measurement of nucleotides in a biofluid; (2) a device and method of recognizing at least one physiological disease or condition based on nucleotide concentrations, ratios, or trends in a biofluid.

    BIOFLUID SENSING DEVICE CYTOKINE MEASUREMENT

    公开(公告)号:US20200080987A1

    公开(公告)日:2020-03-12

    申请号:US16344871

    申请日:2017-10-25

    IPC分类号: G01N33/487 G16H50/30

    摘要: The present disclosure provides a method of using a wearable biofluid sensing device to develop a cytokine profile for an individual. The method includes taking concentration, ratio, and trend measurements of one or more cytokines in the individual's sweat, along with other contemporaneous device measurements to inform sweat rate, skin temperature, sweat sample pH, or other factors. The method further considers these measured values in the context of external information about the individual, and uses such information to develop (1) a baseline cytokine profile characterizing the individual's healthy cytokine levels, or (2) an inflammation profile for a physiological condition, which characterizes the expected cytokine levels for a physiological condition. Also included is a method to use a biofluid sensing device to determine whether an individual has a physiological condition by comparing device measurements to the baseline profile and inflammation profile. Results are then communicated to a device user.

    BIOFLUID SENSING DEVICES WITH SENSOR ABRASION PROTECTION

    公开(公告)号:US20190254641A1

    公开(公告)日:2019-08-22

    申请号:US16403094

    申请日:2019-05-03

    IPC分类号: A61B10/00 A61B5/145 A61B5/00

    摘要: The disclosed invention provides a biofluid sensing device configured with a membrane-enhanced sensor located in a sweat collector. The disclosed analyte-specific sensor is configured to reduce required biofluid sample volume due to its close proximity to the skin and source of sweat biofluid. The sensor is contained within a pH and salinity-stabilized fluid that, in turn, is contained in a selectively permeable membrane to improve sensor performance in variable biofluids, and to protect the sensor from skin contact. In one embodiment, the biofluid sensing device includes means to protect the self-aligning sensors from abrasion against the skin or device components. Other embodiments of the disclosed invention include a track-etched membrane to provide sensor protection.

    CALIBRATION FOR EAB BIOFLUID SENSORS
    9.
    发明申请

    公开(公告)号:US20190142311A1

    公开(公告)日:2019-05-16

    申请号:US16163011

    申请日:2018-10-17

    IPC分类号: A61B5/145 A61B5/1495 A61B5/00

    摘要: Devices for calibrating electrochemical aptamer-based biofluid sensors. In some embodiments a calibrant is located proximate to the EAB sensors, and calibration is facilitated by introduction of the biofluid into the device. Other embodiments include various mechanisms for introducing calibration solution to the sensors, including drawing calibration solution into contact with sensors through electrical potential, a dispensing mechanism such as an ink jet-type nozzle, as well as pressure actuated calibration dispensing. Also included are embodiments employing bifurcated biofluid transport paths that allow alternately for calibration and biofluid sensing.

    HEXAGONAL NANOFLUIDIC MICROCHANNELS FOR BIOFLUID SENSING DEVICES

    公开(公告)号:US20190142309A1

    公开(公告)日:2019-05-16

    申请号:US16206095

    申请日:2018-11-30

    IPC分类号: A61B5/1477 A61B5/00 A61B5/145

    摘要: The disclosed invention provides a biofluid collection device configured with an open microfluidic network, which facilitates nanoliter-scale biofluid collection and transport for biosensing applications. In one embodiment, a biofluid sensing device placed on the skin for measuring a characteristic of an analyte in sweat includes one or more biofluid sensors and a hexagonal open microfluidic network biofluid collector. The disclosed collector provides a volume-reduced pathway for sweat biofluid between the one or more sensors and sweat glands when the device is positioned on the skin. In another embodiment, a biofluid collector includes a network of microchannels comprising three or more repeatedly intersecting channels that provide redundant pathways for biofluid transport. Embodiments of the disclosed invention are also directed to highly stable peptide-based self-assembled monolayers (SAM) and methods of making the SAMs. In some embodiments, the peptide-based SAM is formed on a component of a biofluid sensing device.