公开(公告)号：US20080118491A1

公开(公告)日：2008-05-22

申请号：US11506891

申请日：2006-08-21

Applicant: Kou-Joan Cheng ,  Yo-Chia Chen

Inventor： Kou-Joan Cheng ,  Yo-Chia Chen

IPC: A61K38/47 ,  C07H21/04 ,  C12N1/10 ,  C12N1/14 ,  C12N5/04 ,  C12N5/06 ,  C12N7/01 ,  C12N9/28 ,  C12N9/36 ,  C12Q1/68

CPC classification number: C12N9/2434 ,  A61K38/47

Abstract: A xylanase gene, denoted xynR8, encoding a novel xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermo-tolerable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the β-1,4-glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.

Abstract translation: 提供了一种称为xynR8的木聚糖酶基因，其编码从单分解的瘤胃微生物获得的新型木聚糖酶（XynR8）。 还提供了xynR8基因，木聚糖酶的DNA序列，该酶是耐热的，并且对具有高活性的木聚糖具有高度特异性。 描述了具有xynR8基因的微生物宿主的转化。 用于降解含木聚糖结构的方法包括通过接触木聚糖酶水解木聚糖的β-1,4-糖苷键，并且进一步解决使用上述水解方法的组合物。

公开(公告)号：US08137945B1

公开(公告)日：2012-03-20

申请号：US13150456

申请日：2011-06-01

Applicant: Rey-Ting Guo ,  Ya-Shan Cheng ,  Tzu-Hui Wu ,  Jian-Wen Huang ,  Hui-Lin Lai ,  Cheng-Yen Lin

Inventor： Rey-Ting Guo ,  Ya-Shan Cheng ,  Tzu-Hui Wu ,  Jian-Wen Huang ,  Hui-Lin Lai ,  Cheng-Yen Lin

IPC: C12N9/42 ,  C12N9/14

CPC classification number: C12N9/2437 ,  C12Y302/01004

Abstract: A thermostable cellulase having increased enzyme activity is disclosed. The cellulase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein the tyrosine at position 61 is substituted with an amino acid selected from the group consisting of phenylalanine, alanine and glycine.

Abstract translation: 公开了具有增加的酶活性的热稳定纤维素酶。 纤维素酶包含SEQ ID NO：2的修饰的氨基酸序列，其中61位的酪氨酸被选自苯丙氨酸，丙氨酸和甘氨酸的氨基酸取代。

公开(公告)号：US08192974B1

公开(公告)日：2012-06-05

申请号：US13153618

申请日：2011-06-06

Applicant: Rey-Ting Guo ,  Jian-Wen Huang ,  Ya-Shan Cheng ,  Tzu-Hui Wu ,  Hui-Lin Lai ,  Cheng-Yen Lin

Inventor： Rey-Ting Guo ,  Jian-Wen Huang ,  Ya-Shan Cheng ,  Tzu-Hui Wu ,  Hui-Lin Lai ,  Cheng-Yen Lin

IPC: C12N9/42

CPC classification number: C12N9/2448

Abstract: A glucanase having increased enzyme activity and thermostability is disclosed. The glucanase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein valine at position 18 is substituted with tyrosine and tryptophan at position 203 is substituted with tyrosine.

Abstract translation: 公开了具有增加的酶活性和热稳定性的葡聚糖酶。 葡聚糖酶包含SEQ ID NO：2的修饰的氨基酸序列，其中18位的缬氨酸被酪氨酸取代，203位的色氨酸被酪氨酸取代。

公开(公告)号：US07635471B2

公开(公告)日：2009-12-22

申请号：US11506891

申请日：2006-08-21

Applicant: Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

Inventor： Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

IPC: A61K38/47 ,  A61K38/54 ,  C12N9/42 ,  C12N9/28 ,  C12N9/36 ,  C07H21/04

CPC classification number: C12N9/2434 ,  A61K38/47

Abstract: A xylanase gene, denoted xynR8, encoding a xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermostable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the β-1,4-glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.

Abstract translation: 提供了一种称为xynR8的木聚糖酶基因，其编码从单分解的瘤胃微生物获得的木聚糖酶（XynR8）。 还提供了xynR8基因，木聚糖酶的DNA序列，该酶是热稳定的，并且对具有高活性的木聚糖具有高度特异性。 描述了具有xynR8基因的微生物宿主的转化。 用于降解含木聚糖结构的方法包括通过接触木聚糖酶水解木聚糖的β-1,4-糖苷键，并且进一步解决使用上述水解方法的组合物。