公开(公告)号：US07615362B2

公开(公告)日：2009-11-10

申请号：US11851522

申请日：2007-09-07

申请人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

发明人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/26 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/2482 ,  C12Y302/01008

摘要： A mutated xylanase gene with a broad pH range of reaction includes a forty-first amino acid or a twenty-first amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the twenty-first amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

摘要翻译： 具有广泛pH范围的反应的变异的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第41位氨基酸或第21位氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第二十一个氨基酸，从而形成突变的木聚糖酶基因。

公开(公告)号：US20080254539A1

公开(公告)日：2008-10-16

申请号：US11851593

申请日：2007-09-07

申请人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

发明人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

IPC分类号： C12N15/01 ,  C07H21/00

CPC分类号： C12N9/2485 ,  C12Y302/01032

摘要： A mutated xylanase gene with high reaction activity includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

摘要翻译： 具有高反应活性的突变的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第五十八个氨基酸或第三十八个氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第三十八个氨基酸，从而形成突变的木聚糖酶基因。

公开(公告)号：US20080254526A1

公开(公告)日：2008-10-16

申请号：US11851522

申请日：2007-09-07

申请人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

发明人： Hsueh-Ling Cheng ,  Yo-Chia Chen ,  Yu-Chuan Chiang

IPC分类号： C12N9/26

CPC分类号： C12N9/2482 ,  C12Y302/01008

摘要： A mutated xylanase gene with a broad pH range of reaction includes a forty-first amino acid or a twenty-first amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the twenty-first amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

摘要翻译： 具有广泛pH范围的反应的变异的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第41位氨基酸或第21位氨基酸，以形成突变的木聚糖酶基因。 位点特异性诱变方法包括以下步骤：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第二十一个氨基酸，从而形成突变的木聚糖酶基因。

公开(公告)号：US07635471B2

公开(公告)日：2009-12-22

申请号：US11506891

申请日：2006-08-21

申请人： Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

发明人： Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

IPC分类号： A61K38/47 ,  A61K38/54 ,  C12N9/42 ,  C12N9/28 ,  C12N9/36 ,  C07H21/04

CPC分类号： C12N9/2434 ,  A61K38/47

摘要： A xylanase gene, denoted xynR8, encoding a xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermostable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the β-1,4-glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.

摘要翻译： 提供了一种称为xynR8的木聚糖酶基因，其编码从单分解的瘤胃微生物获得的木聚糖酶（XynR8）。 还提供了xynR8基因，木聚糖酶的DNA序列，该酶是热稳定的，并且对具有高活性的木聚糖具有高度特异性。 描述了具有xynR8基因的微生物宿主的转化。 用于降解含木聚糖结构的方法包括通过接触木聚糖酶水解木聚糖的β-1,4-糖苷键，并且进一步解决使用上述水解方法的组合物。

公开(公告)号：US07749743B2

公开(公告)日：2010-07-06

申请号：US11851593

申请日：2007-09-07

申请人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

发明人： Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Yu-Chuan Chiang

IPC分类号： C12N9/24

CPC分类号： C12N9/2485 ,  C12Y302/01032

摘要： An isolated xylanase gene with mutations includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the isolated xylanase gene. A site-specific mutagenesis method includes: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the isolated xylanase gene.

摘要翻译： 具有突变的分离的木聚糖酶基因包括从将天冬酰胺转化为天冬氨酸产生的第五十八个氨基酸或第三十八个氨基酸，以形成分离的木聚糖酶基因。 位点特异性诱变方法包括：通过将天冬酰胺转化为天冬氨酸来突变木聚糖酶基因的第四十一个氨基酸或第三十八个氨基酸，从而形成分离的木聚糖酶基因。