公开(公告)号：US5500374A

公开(公告)日：1996-03-19

申请号：US107122

申请日：1993-08-17

Applicant: Hu Wenzhi

Inventor： Hu Wenzhi

IPC: B01J20/283 ,  G01N30/02 ,  G01N30/46 ,  G01N30/74 ,  G01N30/88

CPC classification number: G01N30/88 ,  G01N2030/027 ,  G01N2030/8813 ,  G01N30/02 ,  G01N30/461 ,  Y10T436/24

Abstract: A rapid, simple and reliable method and device for diagnosing diabetes mellitus under no influence of diet. The method for diagnosing diabetes mellitus involves injecting a saliva sample from a patient suspected of having diabetes mellitus into a stationary phase in a separation column, thereafter eluting a phosphate buffer as an eluent to separate analytes contained in the saliva sample, subsequently detecting the individual separated analytes with a UV detector and electrostatic ion chromatography to produce a chromatogram, and then diagnosing diabetes mellitus based on the presence or absence of a chromato-peak of the diabetes mellitus-specific component. A zwitterionic stationary phase is employed as the stationary phase and contains a support carrier and a zwitterionic charged layer formed by coating a compound having an ammonium salt portion, a sulfonate ion portion or carboxylate ion portion on the surface of the support carrier.

Abstract translation: 一种用于在不影响饮食的情况下诊断糖尿病的快速，简单和可靠的方法和装置。 用于诊断糖尿病的方法包括将来自疑似患有糖尿病的患者的唾液样品从分离柱中注入静止期，然后洗脱磷酸盐缓冲液作为洗脱液，以分离唾液样品中包含的分析物，随后检测个体分离 使用UV检测器和静电离子色谱法分析物，以产生色谱图，然后根据糖尿病特异性成分的色谱峰的存在或不存在来诊断糖尿病。 使用两性离子固定相作为固定相，并且含有载体载体和通过在载体载体的表面上涂布具有铵盐部分，磺酸根离子部分或羧酸根离子部分的化合物形成的两性离子带电层。

公开(公告)号：US5589069A

公开(公告)日：1996-12-31

申请号：US661892

申请日：1996-06-12

Applicant: Hu Wenzhi

Inventor： Hu Wenzhi

IPC: G01N30/02 ,  B01D15/08 ,  B01J20/286 ,  B01J20/32 ,  G01N30/64 ,  G01N30/74 ,  G01N30/88 ,  G01N30/96

CPC classification number: G01N30/96 ,  B01D15/364 ,  B01J20/2804 ,  B01J20/289 ,  B01J20/29 ,  B01J20/3204 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3285 ,  B01J20/3289 ,  B01D15/3833 ,  B01J2220/54 ,  B01J2220/58 ,  G01N2030/8868 ,  G01N30/02

Abstract: The present invention relates to a method for separating and analyzing ions by electrostatic ion chromatography using water as the eluent not only for the analysis of the group of anions or cations but also for the simultaneous and simple analysis of the two groups of analyte ions. A solid phase packed in a separation column to be used therefor is a zwitterionic stationary phase, comprising a support carrier and a zwitterionic layer formed by directly or indirectly coating or immobilized a compound having both of a positive-charge portion and a negative-charge portion (an ammonium salt or amide portion, and a sulfonate ion portion or carboxylate ion portion) on the surface of the support carrier for detecting given separated ions by ultraviolet method etc. And another present invention relates to a method for separating and analyzing inorganic and organic components etc. simultaneously by multi-functional liquid chromatography.

Abstract translation: 本发明涉及一种通过静电离子色谱法分离和分析离子的方法，使用水作为洗脱液，不仅用于阴离子或阳离子的分析，而且用于同时和简单地分析两组分析物离子。 填充在用于其的分离柱中的固相是两性离子固定相，其包含支撑载体和通过直接或间接涂覆或固定化具有正电荷部分和负电荷部分的化合物形成的两性离子层 （铵盐或酰胺部分，磺酸根离子部分或羧酸根离子部分），用于通过紫外线法检测给定的分离离子。另外本发明涉及一种分离和分析无机和有机物的方法 组分等通过多功能液相色谱同时进行。

公开(公告)号：US5484733A

公开(公告)日：1996-01-16

申请号：US108289

申请日：1993-08-19

Applicant: Hu Wenzhi

Inventor： Hu Wenzhi

IPC: B01J20/283 ,  G01N30/02 ,  G01N30/58 ,  G01N30/74 ,  G01N30/88

CPC classification number: G01N30/58 ,  G01N2030/484 ,  G01N30/02 ,  Y10T436/25

Abstract: The present invention relates to a rapid, simple and reliable method and a device for diagnosing rheumatism under no influence of diet. The method for diagnosing rheumatism includes injecting the urine sample from a suspected case of rheumatism into one end of a solid phase packed in a separation column 3, thereafter eluting a phosphate buffer as an eluent to separate the analytes contained in the urine sample, subsequently detecting the individual separated analytes with a detector 4 to confirm that a predetermined chromatogram is demonstrated by liquid chromatography, the chromatogram developing a higher chromato-peak of the rheumatism-specific component contained in the urine of the patient compared with a chromato-peak of non-rheumatic individual, and establishing the diagnosis of rheumatism on the basis of the presence of the higher peak, wherein a negative/positive charged micellar stationary phase is employed as the stationary phase, including a support carrier and a zwitterionic layer formed by directly or indirectly coating CHAPS and/or CHAPSO micelle on the surface of the support carrier.

Abstract translation: 本发明涉及快速，简单和可靠的方法和用于诊断不受饮食影响的风湿病的装置。 用于诊断风湿病的方法包括将来自疑似风湿病的尿液样品注入装在分离塔3中的固相的一端，然后洗脱磷酸盐缓冲液作为洗脱液，分离尿样中所含的分析物，随后检测 用检测器4分离分离出的分析物，以确认通过液相色谱法证实了预定的色谱图，色谱图显示了患者尿液中包含的风湿性特异性成分的较高色谱峰与非 - 风湿性个体，并根据高峰的存在建立风湿病的诊断，其中使用负/正电荷的胶束固定相作为固定相，包括支持载体和通过直接或间接涂覆形成的两性离子层 CHAPS和/或CHAPSO胶束在载体载体的表面上。