公开(公告)号：US11740174B2

公开(公告)日：2023-08-29

申请号：US17559379

申请日：2021-12-22

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14 ,  G01N15/10

CPC分类号： G01N15/14 ,  G01N15/147 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1436 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/149 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2015/1488

摘要： Described herein are apparatuses and methods for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and quantifying a relative contribution of each component to the supercurve.

公开(公告)号：US20210231554A1

公开(公告)日：2021-07-29

申请号：US17209057

申请日：2021-03-22

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14

摘要： Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US09551645B2

公开(公告)日：2017-01-24

申请号：US14793626

申请日：2015-07-07

申请人： Kinetic River Corp.

发明人： Giacomo Vacca

IPC分类号： G01N15/14 ,  G01N15/10

CPC分类号： G01N15/1436 ,  G01N15/1404 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149

摘要： A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

摘要翻译： 一种粒子分析仪，包括基本上非衍射光束的源; 流路，被配置为在流动池中产生具有至少4的横截面纵横比和至少50微米的最大横截面尺寸的带状芯流; 所述流动池被配置为将所述核心流的一部分暴露于所述光束; 检测器，被配置为接收由核心流中的粒子与光束的相互作用产生的信号; 与所述流动池连接的第一分选致动器，在所述暴露的核心流的段的下游; 与所述流动路径和所述第一致动器的下游流体连接的多个分选通道; 所述致动器具有多个致动状态，每个状态被配置为将所述核心流的至少一部分引导到相应的通道; 与所述流通池连接的第二分选致动器，与所述第一致动器相对，并且与所述第一致动器协调地可操作。

公开(公告)号：US11536642B2

公开(公告)日：2022-12-27

申请号：US17328331

申请日：2021-05-24

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14 ,  G01N15/10

摘要： Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20220113239A1

公开(公告)日：2022-04-14

申请号：US17559379

申请日：2021-12-22

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14 ,  G01N15/10

摘要： Described herein are apparatuses and methods for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and quantifying a relative contribution of each component to the supercurve.

公开(公告)号：US11249004B2

公开(公告)日：2022-02-15

申请号：US17209057

申请日：2021-03-22

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14 ,  G01N15/10

摘要： Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20210341391A1

公开(公告)日：2021-11-04

申请号：US17363907

申请日：2021-06-30

申请人： Kinetic River Corp.

发明人： Giacomo Vacca

IPC分类号： G01N21/65 ,  G01N15/14

摘要： An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles.

公开(公告)号：US20210302301A1

公开(公告)日：2021-09-30

申请号：US17346893

申请日：2021-06-14

申请人： Kinetic River Corp.

发明人： Giacomo Vacca

IPC分类号： G01N15/14

摘要： A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

公开(公告)号：US20210278332A1

公开(公告)日：2021-09-09

申请号：US17326662

申请日：2021-05-21

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14

摘要： Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20210270716A1

公开(公告)日：2021-09-02

申请号：US17307975

申请日：2021-05-04

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14

摘要： Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.