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    Chemically modified G-CSF
    2.
    发明申请
    Chemically modified G-CSF 审中-公开

    公开(公告)号：US20070219357A1

    公开(公告)日：2007-09-20

    申请号：US11716866

    申请日：2007-03-12

    申请人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

    发明人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

    IPC分类号： C07K14/475

    CPC分类号： C07K17/08 ,  A61K38/00 ,  A61K47/60 ,  C07K14/535 ,  Y10S930/145

    摘要： The present invention provides a chemically-modified protein prepared by binding polyethylene glycol to a polypeptide characterized by being the product of expression by a host cell of an exogenous DNA sequence and substantially having the following amino acid sequence: (Met)n Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro (n = 0 (SEQ ID NO: 1) or 1 (SEQ ID NO: 2)) The chemically-modified protein according to the present invention has a neutrophils-increasing activity much more lasted than that of the intact human G-CSF, enabling fewer numbers of administration with a lower dose.

    Production of erthropoietin
    3.
    发明授权
    Production of erthropoietin 失效
    生产促红细胞生成素

    公开(公告)号：US5955422A

    公开(公告)日：1999-09-21

    申请号：US100197

    申请日：1993-08-02

    申请人： Fu-Kuen Lin

    发明人： Fu-Kuen Lin

    IPC分类号： A61K38/00 ,  A61K38/16 ,  C07K14/505 ,  C07K16/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

    CPC分类号： C07K14/505 ,  C07K16/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  A61K38/00 ,  C12N2800/108 ,  Y10S530/835

    摘要： Disclosed are novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of mammalian erythropoietin ("EPO") which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence. Illustratively, genomic DNA, cDNA and manufactured DNA sequences coding for part or all of the sequence of amino acid residues of EPO or for analogs thereof are incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture. Upon isolation from culture media or cellular lysates or fragments, products of expression of the DNA sequences display, e.g., the immunological properties and in vitro and in vivo biological activities of EPO of human or monkey species origins. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of EPO. Also disclosed are improved methods for the detection of specific single stranded polynucleotides in a heterologous cellular or viral sample prepared from, e.g., DNA present in a plasmid or viral-borne cDNA or genomic DNA "library".

    摘要翻译： 公开了具有部分或全部主要结构构象和哺乳动物促红细胞生成素（“EPO”）的一种或多种生物学特性的新型多肽，其特征在于优选形式，其为外源DNA序列的原核或真核宿主表达的产物 。 说明性地，编码EPO或其类似物的氨基酸残基序列的部分或全部的基因组DNA，cDNA和制备的DNA序列被掺入用于转化或转染合适的原核或真核宿主细胞的自主复制质粒或病毒载体，例如 细菌，酵母或脊椎动物细胞。 当从培养基或细胞裂解物或片段分离时，DNA序列的表达产物显示例如人或猴物种来源的EPO的免疫学特性和体外和体内生物学活性。 还公开了共享EPO的生物化学和免疫学性质的化学合成的多肽。 还公开了用于检测由例如存在于质粒或病毒携带的cDNA或基因组DNA“文库”中的DNA制备的异源细胞或病毒样品中特异性单链多核苷酸的改进方法。

    Chemically-modified G-CSF
    7.
    发明申请
    Chemically-modified G-CSF 审中-公开

    公开(公告)号：US20020177688A1

    公开(公告)日：2002-11-28

    申请号：US09921114

    申请日：2001-08-02

    申请人： Kirin-Amgen, Inc.,

    发明人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

    IPC分类号： A61K038/17 ,  C07K014/435

    CPC分类号： C07K17/08 ,  A61K38/00 ,  A61K47/60 ,  C07K14/535

    摘要： The present invention provides a chemically-modified protein prepared by binding polyethylene glycol to a polypeptide characterized by being the product of expression by a host cell of an exogenous DNA sequence and substantially having the following amino acid sequence: 1 nullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnull(Met) n Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Glnnullnull Ser Phe Leu Leu Lys Cys Leu Glu Gln Val Argnullnull Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glunullnull Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pronullnull Glu Glu Leu Val Leu Leu Gly His Ser Leu Glynullnull Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Sernullnull Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Glnnullnull Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leunullnull Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leunullnull Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Valnullnull Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Hetnullnull Glu Glu Leu Gly Met Ala Pro Ala Leu Gln Pronullnull Thr Gln Gly Ala Het Pro Ala Phe Ala Ser Alanullnull Phe Gln Arg Arg Ala Gly Gly Val Leu Val Alanullnull Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyrnullnull Arg Val Leu Arg His Leu Ala Gln Pronullnullnullnullnullnullnullnullnullnull (n nullnull0 or 1) The chemically-modified protein according to the present invention has a neutrophils-increasing activity much more lasted than that of the intact human G-CSF, enabling fewer numbers of administration with a lower dose.

    Chemically modified G-CSF
    9.
    发明申请
    Chemically modified G-CSF 审中-公开

    公开(公告)号：US20060247422A1

    公开(公告)日：2006-11-02

    申请号：US11342519

    申请日：2006-01-30

    申请人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

    发明人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

    IPC分类号： C07K14/53

    CPC分类号： C07K17/08 ,  A61K38/00 ,  A61K47/60 ,  C07K14/535

    摘要： The present invention provides a chemically-modified protein prepared by binding polyethylene glycol to a polypeptide characterized by being the product of expression by a host cell of an exogenous DNA sequence and substantially having the following amino acid sequence: (Het)n Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala Thr Thr Ile Trp Gln Gln Het Glu Glu Leu Gly Het Ala Pro Ala Leu Gln Pro Thr Gln Gly Ala Het Pro Ala Phe Ala Ser Ala Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro (n = 0 or 1)

    Chemically modified G-CSF
    10.
    发明申请
    Chemically modified G-CSF 审中-公开
    化学修饰的G-CSF

    公开(公告)号：US20040158041A1

    公开(公告)日：2004-08-12

    申请号：US10750797

    申请日：2004-01-02

    申请人： KIRIN-AMGEN, INC.

    发明人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

    IPC分类号： C07K014/47

    CPC分类号： C07K17/08 ,  A61K38/00 ,  A61K47/60 ,  C07K14/535

    摘要： The present invention provides a chemically-modified protein prepared by binding polyethylene glycol to a polypeptide characterized by being the product of expression by a host cell of an exogenous DNA sequence and substantially having the following amino acid sequence: 1 (Met)n ThrProLeuGlyProAlaSerSerLeuProGln SerPheLeuLeuLysCysLeuGluGlnValArg LysIleGlnGlyAspGlyAlaAlaLeuGlnGlu LysLeuCysAlaThrTyrLysLeuCysHisPro GluGluLeuValLeuLeuGlyHisSerLeuGly IleProTrpAlaProLeuSerSerCysProSer GlnAlaLeuGlnLeuAlaGlyCysLeuSerGln LeuHisSerGlyLeuPheLeuTyrGlnGlyLeu LeuGlnAlaLeuGluGlyIleSerProGluLeu GlyProThrLeuAspThrLeuGlnLeuAspVal AlaAspPheAlaThrThrIleTrpGlnGlnMet GluGluLeuGlyMetAlaProAlaLeuGlnPro ThrGlnGlyAlaMetProAlaPheAlaSerAla PheGlnArgArgAlaGlyGlyValLeuValAla SerHisLeuGlnSerPheLeuGluValSerTyr ArgValLeuArgHisLeuAlaGlnPro (n null 0 or 1) The chemically-modified protein according to the present invention has a neutrophils-increasing activity much more lasted than that of the intact human G-CSF, enabling fewer numbers of administration with a lower dose.

    摘要翻译： 本发明提供通过将聚乙二醇与多肽结合而制备的化学修饰的蛋白质，其特征在于宿主细胞表达外源DNA序列并基本上具有以下氨基酸序列的产物：根据 本发明具有比完整的人G-CSF更持久的中性粒细胞增加活性，使得较少剂量的给药较少。