公开(公告)号：US20240216881A1

公开(公告)日：2024-07-04

申请号：US18577250

申请日：2022-06-22

申请人： THE JAPAN STEEL WORKS, LTD.

发明人： Naoki UEDA ,  Satoru NAKAMURA ,  Kenichi FRURKI

IPC分类号： B01F33/80 ,  B01F23/60 ,  B01F27/721 ,  B01F33/40 ,  F27D3/00 ,  F27D3/08 ,  H01M10/056 ,  H01M10/058

CPC分类号： B01F33/8052 ,  B01F23/69 ,  B01F27/721 ,  B01F33/402 ,  B01F33/404 ,  F27D3/08 ,  H01M10/056 ,  H01M10/058 ,  F27D2003/0083

摘要： In a reaction apparatus, a reaction furnace is cylindrical and includes a supply port for receiving a raw material to be supplied at one end and a discharge port for discharging a reaction product at another end. A temperature control region includes an apparatus for controlling a temperature of the reaction furnace. A screw extends from the one end of the reaction furnace to the other end and is configured to be able to convey the raw material toward the discharge port by rotating. A first fluid control region includes a first fluid inlet and outlet for allowing a first fluid to pass through the reaction furnace in a predetermined region in the intermediate part. A second fluid control region includes a second fluid inlet and outlet for allowing a second fluid to pass through a region different from the first fluid control region in the intermediate part.

公开(公告)号：US20240226826A1

公开(公告)日：2024-07-11

申请号：US18559309

申请日：2022-03-24

申请人： ADVACCINE (SUZHOU) BIOPHARMACEUTICALS CO. LTD.

发明人： Hongkai Yang ,  Zongsheng Wu

IPC分类号： B01F29/25 ,  B01F33/40 ,  C12N1/06 ,  C12N15/10

CPC分类号： B01F29/25 ,  B01F33/404 ,  C12N1/06 ,  C12N15/1017

摘要： Provided is a method for extracting plasmid DNA in bacteria, realizing lysis and neutralization during plasmid production in two mixing assemblies connected in series, and comprising the following steps: (1) mixing, (2) lysing, and (3) neutralizing. Step (1) is completed in a first mixing assembly; step (2) is completed in a lysis helical tube; step (3) is completed in a second mixing assembly; and the first mixing assembly, the lysis helical tube and the second mixing assembly are sequentially connected in series. A device used in the plasmid preparation process is simple, is convenient to operate, is low in costs, can remove a large amount of impurities during cell lysis without professional customized device and expensive device, has safe components, realizes automatic continuous lysis, and facilitates industrial production.