公开(公告)号：US20170211113A1

公开(公告)日：2017-07-27

申请号：US15114250

申请日：2014-01-28

申请人： PepsiCo, Inc.

发明人： Junhua TAO ,  Guoqing LI ,  Xiaoliang LIANG ,  Thomas LEE ,  Gregory YEP ,  Maoqi HOU ,  Andrew TAO

IPC分类号： C12P19/56

CPC分类号： C12P19/56 ,  C12Y204/01013 ,  C12Y204/01051

摘要： Provided is a method for preparing Rebaudioside M by using an enzyme method. In the method, Rebaudioside A or Rebaudioside D is used as a substrate, and in the presence of sucrose and UDP, Rebaudioside M is generated by means of reaction of the substrate under the catalysis of a mixture of UDP-glucosyl transferase and sucrose synthetase, or recombinant cells containing the UDP-glucosyl transferase and sucrose synthetase. The reaction is carried out in an aqueous-phase system at a temperature of 20 to 60° C. and pH 5.0 to 9.0. The reaction system further contains dimethyl sulfoxide at a concentration of 3% to 5% according to the ratio by volume for facilitating solubilization of the substrate.