METHOD FOR PREPARING REBAUDIOSIDE M BY USING ENZYME METHOD

    公开(公告)号:US20170211113A1

    公开(公告)日:2017-07-27

    申请号:US15114250

    申请日:2014-01-28

    申请人: PepsiCo, Inc.

    IPC分类号: C12P19/56

    摘要: Provided is a method for preparing Rebaudioside M by using an enzyme method. In the method, Rebaudioside A or Rebaudioside D is used as a substrate, and in the presence of sucrose and UDP, Rebaudioside M is generated by means of reaction of the substrate under the catalysis of a mixture of UDP-glucosyl transferase and sucrose synthetase, or recombinant cells containing the UDP-glucosyl transferase and sucrose synthetase. The reaction is carried out in an aqueous-phase system at a temperature of 20 to 60° C. and pH 5.0 to 9.0. The reaction system further contains dimethyl sulfoxide at a concentration of 3% to 5% according to the ratio by volume for facilitating solubilization of the substrate.

    Enzymatic Method for Preparing Rebaudioside M
    3.
    发明申请
    Enzymatic Method for Preparing Rebaudioside M 审中-公开
    芦丁苷M的制备方法

    公开(公告)号:US20160298159A1

    公开(公告)日:2016-10-13

    申请号:US14911876

    申请日:2013-09-29

    申请人: PEPSICO INC.

    IPC分类号: C12P19/56 C12P19/18

    摘要: Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.

    摘要翻译: 提供了使用酶法制备新蛇菊苷M的方法。 在该方法中,使用新蛇菊苷A或莱鲍迪甙D作为底物; 并且在葡糖基供体的存在下,通过在UDP-葡糖基转移酶的催化下和/或含有UDP-葡糖基转移酶的重组细胞的底物反应产生新蛇菊苷M。