公开(公告)号：US07619265B2

公开(公告)日：2009-11-17

申请号：US10577734

申请日：2004-11-05

申请人： Timothy Ashley ,  Kevin M Brunson ,  Philip D Buckle ,  Timothy I Cox ,  Norman J Geddes ,  John H Jefferson ,  Russell A Noble ,  Ian C Sage ,  David J Combes

发明人： Timothy Ashley ,  Kevin M Brunson ,  Philip D Buckle ,  Timothy I Cox ,  Norman J Geddes ,  John H Jefferson ,  Russell A Noble ,  Ian C Sage ,  David J Combes

IPC分类号： H01L21/00

CPC分类号： H01L51/0508 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2400/0633 ,  B82Y10/00 ,  G01N27/414 ,  H01L51/0035 ,  H01L51/0052 ,  H01L51/0595 ,  Y10S977/807

摘要： A molecular single electron transistor (MSET) detector device (14) is described that comprises at least one organic molecule (87) connecting a drain electrode (84) and a source electrode (82). In use, said at least one organic molecule (87) provides a quantum confinement region. At least one analyte receptor site (90, 92) is provided in the vicinity of said at least one organic molecule (87) that bind molecules of interest (analytes). A fluid analyser (2) is also described that includes the MSET detector, a pre-concentrator (4) and a fluid gating structure (6). The fluid gating structure (6) is arranged to selectively route fluid from the pre-concentrator (4) to either one of the detector (14) and an exhaust port (12). The pre-concentrator (4), fluid gating structure (6) and detector (14) are each formed as substantially planar layers and arranged in a stack or cube.

摘要翻译： 描述了分子单电子晶体管（MSET）检测器装置（14），其包括连接漏电极（84）和源电极（82）的至少一个有机分子（87）。 在使用中，所述至少一种有机分子（87）提供量子限制区域。 在所述至少一个结合感兴趣的分子（分析物）的有机分子（87）附近提供至少一个分析物受体位点（90,92）。 还描述了包括MSET检测器，预浓缩器（4）和流体门控结构（6）的流体分析器（2）。 流体门控结构（6）布置成选择性地将流体从预浓缩器（4）引导到检测器（14）和排气口（12）中的任一个。 预浓缩器（4），流体门控结构（6）和检测器（14）各自形成为基本上平面的层并且布置成堆叠或立方体。

公开(公告)号：US08841085B2

公开(公告)日：2014-09-23

申请号：US13060902

申请日：2009-08-27

申请人： Ick-Chan Kwon ,  Kui-Won Choi ,  Kwang-Meyung Kim ,  In-Chan Youn ,  Seul-Ki Lee ,  Kyeong-Soon Park ,  Dae-Hyuk Moon ,  Dae-Yoon Chi ,  Seung-Jin Lee ,  Seung-Jae Myung

发明人： Ick-Chan Kwon ,  Kui-Won Choi ,  Kwang-Meyung Kim ,  In-Chan Youn ,  Seul-Ki Lee ,  Kyeong-Soon Park ,  Dae-Hyuk Moon ,  Dae-Yoon Chi ,  Seung-Jin Lee ,  Seung-Jae Myung

IPC分类号： C12Q1/34 ,  C12Q1/37 ,  G01N33/543 ,  C12Q1/04 ,  C12Q1/06

CPC分类号： C12Q1/37 ,  G01N33/54346 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/807 ,  Y10S977/811 ,  Y10S977/953

摘要： Disclosed are a nanoparticle sensor for measuring protease activity, for protease imaging, and a method for preparing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and a quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis and dementia.

摘要翻译： 公开了用于测定蛋白酶活性的蛋白酶成像用纳米粒子传感器及其制备方法。 更具体地，本发明涉及用于测量蛋白酶活性的纳米颗粒传感器，其中荧光团和猝灭剂缀合的肽底物与生物相容性聚合物纳米颗粒缀合。 肽底物被蛋白酶特异性裂解。 根据本发明的传感器能​​够抑制荧光材料上猝灭剂具有高灭绝活性的荧光发射。 但是如果肽底物被特异性蛋白酶裂解，则强荧光是特异性发射的。 因此，该传感器作为筛选新型药物如蛋白酶过表达抑制剂的方法，以及早期诊断不治之症以及各种疾病，如自身免疫性疾病，包括癌症，骨关节炎，类风湿性关节炎和痴呆症，都是特别有用的。

公开(公告)号：US20110213121A1

公开(公告)日：2011-09-01

申请号：US13060902

申请日：2009-08-27

申请人： Ick-Chan Kwon ,  Kui-Won Choi ,  Kwang-Meyung Kim ,  In-Chan Youn ,  Seul-Ki Lee ,  Kyeong-Soon Park ,  Dae-Hyuk Moon ,  Dae-Yoon Chi ,  Seung-Jin Lee ,  Seung-Jae Myung

发明人： Ick-Chan Kwon ,  Kui-Won Choi ,  Kwang-Meyung Kim ,  In-Chan Youn ,  Seul-Ki Lee ,  Kyeong-Soon Park ,  Dae-Hyuk Moon ,  Dae-Yoon Chi ,  Seung-Jin Lee ,  Seung-Jae Myung

IPC分类号： C07K9/00 ,  B82Y5/00

CPC分类号： C12Q1/37 ,  G01N33/54346 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/807 ,  Y10S977/811 ,  Y10S977/953

摘要： Disclosed are a nanoparticle sensor for measuring protease activity, for protease imaging, and a method for preparing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and a quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis and dementia.

摘要翻译： 公开了用于测定蛋白酶活性的蛋白酶成像用纳米粒子传感器及其制备方法。 更具体地，本发明涉及用于测量蛋白酶活性的纳米颗粒传感器，其中荧光团和猝灭剂缀合的肽底物与生物相容性聚合物纳米颗粒缀合。 肽底物被蛋白酶特异性裂解。 根据本发明的传感器能​​够抑制荧光材料上猝灭剂具有高灭绝活性的荧光发射。 但是如果肽底物被特异性蛋白酶裂解，则强荧光是特异性发射的。 因此，该传感器作为筛选新型药物如蛋白酶过表达抑制剂的方法，以及早期诊断不治之症以及各种疾病，如自身免疫性疾病，包括癌症，骨关节炎，类风湿性关节炎和痴呆症，都是特别有用的。

公开(公告)号：US20070134667A1

公开(公告)日：2007-06-14

申请号：US10577734

申请日：2004-11-05

申请人： Timothy Ashley ,  Kevin Brunson ,  Philip Buckle ,  Timothy Cox ,  Norman Geddes ,  John Jefferson ,  Russell Noble ,  Ian Sage ,  David Combes

发明人： Timothy Ashley ,  Kevin Brunson ,  Philip Buckle ,  Timothy Cox ,  Norman Geddes ,  John Jefferson ,  Russell Noble ,  Ian Sage ,  David Combes

IPC分类号： C12Q1/68 ,  H01L51/00 ,  C12M3/00

CPC分类号： H01L51/0508 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2400/0633 ,  B82Y10/00 ,  G01N27/414 ,  H01L51/0035 ,  H01L51/0052 ,  H01L51/0595 ,  Y10S977/807

摘要： A molecular single electron transistor (MSET) detector device (14) is described that comprises at least one organic molecule (87) connecting a drain electrode (84) and a source electrode (82). In use, said at least one organic molecule (87) provides a quantum confinement region. At least one analyte receptor site (90, 92) is provided in the vicinity of said at least one organic molecule (87) that bind molecules of interest (analytes). A fluid analyser (2) is also described that includes the MSET detector, a pre-concentrator (4) and a fluid gating structure (6). The fluid gating structure (6) is arranged to selectively route fluid from the pre-concentrator (4) to either one of the detector (14) and an exhaust port (12). The pre-concentrator (4), fluid gating structure (6) and detector (14) are each formed as substantially planar layers and arranged in a stack or cube.

摘要翻译： 描述了分子单电子晶体管（MSET）检测器装置（14），其包括连接漏电极（84）和源电极（82）的至少一个有机分子（87）。 在使用中，所述至少一种有机分子（87）提供量子限制区域。 在所述至少一个结合感兴趣的分子（分析物）的有机分子（87）附近提供至少一个分析物受体位点（90,92）。 还描述了包括MSET检测器，预浓缩器（4）和流体门控结构（6）的流体分析器（2）。 流体门控结构（6）布置成选择性地将流体从预浓缩器（4）引导到检测器（14）和排气口（12）中的任一个。 预浓缩器（4），流体门控结构（6）和检测器（14）各自形成为基本上平面的层并且布置成堆叠或立方体。