公开(公告)号：US06467630B1

公开(公告)日：2002-10-22

申请号：US09653432

申请日：2000-09-01

申请人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

发明人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

IPC分类号： B03B560

CPC分类号： B03C5/026 ,  B01D39/1692 ,  B01D39/18 ,  B01D49/00 ,  B01D57/02 ,  B01D61/00 ,  B01D61/14 ,  G01N27/44769 ,  G01N30/0005

摘要： The present invention provides apparatuses and methods for continuously separating particles of interest from a heterogeneous particle population. The heterogeneity can be based on, for example, magnetic susceptibility, particle size, thermal diffusion, phase solubility, and combinations of the preceding. Based on the heterogeneity, a separation force capable of exploiting the separand (i.e., particles subject to the separation process) is provided. The various embodiments of the present invention preferably employ an annular separation channel, appropriate separation force and flow compartments. In a first embodiment, an annular separation channel having semi-permeable inner and outer cylindrical walls is used to generate lateral convection forces. In a second embodiment of the present invention, an annular separation channel having heat conductive inner and outer cylindrical walls is used to generate thermal diffusion forces. In a third embodiment, an annular separation channel having non-permeable inner and outer cylindrical walls is used to generate a solubility difference separation force based on solubility coefficient differences between phases of flow compartments. In a fourth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate an electrophoretic separation force. In a fifth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate a dielectrophoretic separation force. Yet other embodiments of the present invention provide for the combination of separation forces including lateral convection, thermal diffusion, solubility difference, electrophoretic, dielectrophoretic, and magnetic separation forces.

摘要翻译： 本发明提供了用于连续分离感兴趣的颗粒与异质颗粒群体的装置和方法。 异质性可以基于例如磁化率，粒度，热扩散，相溶性和前述的组合。 基于异质性，提供能够利用分离的分离力（即，进行分离处理的颗粒）。 本发明的各种实施例优选地采用环形分离通道，适当的分离力和流动隔室。 在第一实施例中，使用具有半透的内圆柱形壁和外圆柱形壁的环形分隔通道产生横向对流力。 在本发明的第二实施例中，使用具有导热内壁和外圆柱壁的环形分隔通道产生热扩散力。 在第三实施例中，使用具有不可渗透的内圆柱形壁和外圆柱形壁的环形分离通道，以基于流动隔室的相位之间的溶解度系数差产生溶度差分离力。 在第四实施例中，使用具有导电内壁和外圆柱形壁的环形分隔通道产生电泳分离力。 在第五实施例中，使用具有导电内壁和外圆柱形壁的环形分隔通道产生介电电泳分离力。 本发明的其它实施例提供了分离力的组合，包括横向对流，热扩散，溶解度差异，电泳，介电电泳和磁力分离力。