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1.发明授权公开(公告)号：US06467630B1
公开(公告)日：2002-10-22
申请号：US09653432
申请日：2000-09-01
申请人： Maciej Zborowski , Jeff Chalmers , Lee R. Moore
发明人： Maciej Zborowski , Jeff Chalmers , Lee R. Moore
IPC分类号： B03B560
CPC分类号： B03C5/026 , B01D39/1692 , B01D39/18 , B01D49/00 , B01D57/02 , B01D61/00 , B01D61/14 , G01N27/44769 , G01N30/0005
摘要： The present invention provides apparatuses and methods for continuously separating particles of interest from a heterogeneous particle population. The heterogeneity can be based on, for example, magnetic susceptibility, particle size, thermal diffusion, phase solubility, and combinations of the preceding. Based on the heterogeneity, a separation force capable of exploiting the separand (i.e., particles subject to the separation process) is provided. The various embodiments of the present invention preferably employ an annular separation channel, appropriate separation force and flow compartments. In a first embodiment, an annular separation channel having semi-permeable inner and outer cylindrical walls is used to generate lateral convection forces. In a second embodiment of the present invention, an annular separation channel having heat conductive inner and outer cylindrical walls is used to generate thermal diffusion forces. In a third embodiment, an annular separation channel having non-permeable inner and outer cylindrical walls is used to generate a solubility difference separation force based on solubility coefficient differences between phases of flow compartments. In a fourth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate an electrophoretic separation force. In a fifth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate a dielectrophoretic separation force. Yet other embodiments of the present invention provide for the combination of separation forces including lateral convection, thermal diffusion, solubility difference, electrophoretic, dielectrophoretic, and magnetic separation forces.
摘要翻译： 本发明提供了用于连续分离感兴趣的颗粒与异质颗粒群体的装置和方法。 异质性可以基于例如磁化率，粒度，热扩散，相溶性和前述的组合。 基于异质性，提供能够利用分离的分离力（即，进行分离处理的颗粒）。 本发明的各种实施例优选地采用环形分离通道，适当的分离力和流动隔室。 在第一实施例中，使用具有半透的内圆柱形壁和外圆柱形壁的环形分隔通道产生横向对流力。 在本发明的第二实施例中，使用具有导热内壁和外圆柱壁的环形分隔通道产生热扩散力。 在第三实施例中，使用具有不可渗透的内圆柱形壁和外圆柱形壁的环形分离通道，以基于流动隔室的相位之间的溶解度系数差产生溶度差分离力。 在第四实施例中，使用具有导电内壁和外圆柱形壁的环形分隔通道产生电泳分离力。 在第五实施例中，使用具有导电内壁和外圆柱形壁的环形分隔通道产生介电电泳分离力。 本发明的其它实施例提供了分离力的组合，包括横向对流，热扩散，溶解度差异，电泳，介电电泳和磁力分离力。
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公开(公告)号：US6120735A
公开(公告)日：2000-09-19
申请号：US352725
申请日：1999-07-13
申请人： Maciej Zborowski , Jeff Chalmers , Lee R. Moore
发明人： Maciej Zborowski , Jeff Chalmers , Lee R. Moore
CPC分类号： G01N33/491 , G01N2015/149
摘要： A method and apparatus for the fraction sorting of cells is provided. The invention include a dispensing device, aspirating device, flow channel and a magnetic assembly. A first fractional sorting device includes a dipole fractional cell sorter. The dipole fractional cell sorter includes large aspect ratio flow channel and a dipole magnetic assembly. A second fractional sorting device includes a quadrupole fractional cell sorter. The quadrupole fractional cell sorter includes an annular flow channel having inlet and outlet splitters, and a quadrupole magnetic assembly. The quadrupole magnetic assembly includes pole pieces having projecting members with convex surfaces and in is in physical communication with the annular flow chamber. The method of the present invention provides a flow through fractional cell sorting process which is based on the application of a magnetic force to cells having a range of magnetic labeling densities.
摘要翻译： 提供了用于细胞分级分选的方法和装置。 本发明包括分配装置，抽吸装置，流动通道和磁性组件。 第一分数分选装置包括偶极子分数细胞分选器。 偶极子分数细胞分选机包括大纵横比流动通道和偶极磁组件。 第二分数分类装置包括四极分数细胞分选器。 四极分级电池分选机包括具有入口和出口分流器以及四极磁组件的环形流动通道。 四极磁组件包括具有凸表面并具有与环形流动腔物理连通的突出构件的极片。 本发明的方法提供了一种通过分数细胞分选过程的流程，其基于将磁力应用于具有一定范围的磁标记密度的细胞。
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公开(公告)号：US5968820A
公开(公告)日：1999-10-19
申请号：US806598
申请日：1997-02-26
申请人： Maciej Zborowski , Jeff Chalmers , Lee R. Moore
发明人： Maciej Zborowski , Jeff Chalmers , Lee R. Moore
IPC分类号： B03C1/035 , C12M1/42 , C12N13/00 , G01N15/14 , G01N33/543 , C12N5/00 , A01N1/02 , B01D35/06 , B03C1/00 , B07C5/02 , B07C9/00
CPC分类号： G01N33/54326 , B03C1/035 , C12M47/04 , C12N13/00 , G01N2015/149
摘要： A method for separating cells is presented. The method provides a flow through fractional cell sorting process that is based on the application of a magnetic force to cells having a range of magnetic labeling densities. Magnetically labeled cells are introduced into a flow channel. The cells are continuously fractionated into flow streams based on magnetic susceptibility by subjecting the cells in the flow channel to a magnetic field. The fractionated cells are recovered based on their response to the magnetic field. Methods employing dipole fractional sorters and quadrapole annular sorters are also presented.
摘要翻译： 提出了一种分离细胞的方法。 该方法提供了通过分数细胞分选过程的流程，其基于将磁力施加到具有一定范围的磁标记密度的细胞。 将磁性标记的细胞引入流动通道。 通过使流路中的细胞经受磁场，基于磁化率将细胞连续分馏成流动流。 基于它们对磁场的响应，分离的细胞被回收。 还提出了使用偶极子分数分拣机和四极环状分拣机的方法。
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公开(公告)号：US06557430B2
公开(公告)日：2003-05-06
申请号：US10187457
申请日：2002-07-01
IPC分类号： G01N2100
CPC分类号： G01P5/18 , G01N15/10 , G01N27/447 , G01N2015/1075 , G01N2015/1081 , G01N2015/149
摘要： The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing region of space having a substantially constant magnetic force field is also provided.
摘要翻译： 本发明提供了用于确定多个粒子物理特性中的至少一个的方法和装置。 颗粒物理特性包括粒度，形状，磁化率，磁性标记密度，电荷分离，介电常数及其衍生物。 该方法包括产生具有基本上恒定的力场的空间区域，通过在至少两个时间上定义的数字图像中识别和定位粒子及其坐标来确定该区域内的至少一个粒子的速度，以及确定粒子物理特性 从确定的速度和预定的力场大小和方向。 描述了一种用于确定一个或多个粒子物理特性的装置，其具有用于使至少一个粒子经受至少一个力场的力场装置，基本上透明的流动通道，以及用于收集和分析与该相关联的数据的数据的计算机系统 至少一个粒子。 提供了一种用于确定一个或多个粒子物理特性的系统，其具有用于产生具有预定力场大小和方向的至少一个力场并且用于使至少一个粒子经受至少一个力场的力场装置， 用于调节将至少一个粒子引入力场设备的系统，以及用于收集和分析与至少一个粒子相关联的数据的计算机系统。 还提供了一种用于产生具有基本恒定的磁力场的空间区域的极片组件。
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公开(公告)号：US6082205A
公开(公告)日：2000-07-04
申请号：US20327
申请日：1998-02-06
IPC分类号： G01N15/10 , G01N15/14 , G01N27/447 , G01P5/18 , G01N21/00
CPC分类号： G01P5/18 , G01N15/10 , G01N27/447 , G01N2015/1075 , G01N2015/1081 , G01N2015/149
摘要： The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing a region of space having a substantially constant magnetic force field is also provided.
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公开(公告)号：US20070020770A1
公开(公告)日：2007-01-25
申请号：US11528231
申请日：2006-09-27
申请人： Aaron Fleischman , Shuvo Roy , Jeff Chalmers , Maciej Zborowski
发明人： Aaron Fleischman , Shuvo Roy , Jeff Chalmers , Maciej Zborowski
IPC分类号： C12M1/34 , G01N33/551 , C12M3/00
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/043 , B01L2400/0487 , C12M47/04 , G01N15/1031 , G01N15/1056 , G01N15/1456 , G01N2015/1006 , G01N2015/1062 , G01N2015/1081
摘要： The present invention provides a MEMS-based integrated particle identification system having a substrate, a magnetic structure, and a bioferrograph. The substrate includes a topside portion, backside portion and a flow system. The flow system includes a flow channel for accepting the flow of a stream of particles to identified. The magnetic structure is in physical communication with the topside and backside portions of the substrate and has at least two pole pieces. A plurality of pole piece embodiments are provided for generating a magnetic field that acts on magnetically susceptible particles in the flow stream. The bioferrograph has at least one sensor for identifying the presence and quantity of magnetically susceptible particles. A plurality of sensor embodiments are also provided.
摘要翻译： 本发明提供了一种基于MEMS的集成粒子识别系统，该系统具有基底，磁性结构和生物计量学。 衬底包括顶侧部分，后侧部分和流动系统。 流动系统包括用于接收所识别的颗粒物流的流动通道。 磁性结构与衬底的顶侧和后侧部分物理连通，并具有至少两个极片。 提供了多个极片实施例，用于产生作用在流动流中的易磁敏颗粒上的磁场。 生物传感器具有至少一个用于识别磁敏感颗粒的存在和数量的传感器。 还提供了多个传感器实施例。
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公开(公告)号：US06412359B1
公开(公告)日：2002-07-02
申请号：US09607702
申请日：2000-06-30
IPC分类号： G01N2100
CPC分类号： G01P5/18 , G01N15/10 , G01N27/447 , G01N2015/1075 , G01N2015/1081 , G01N2015/149
摘要： The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing a region of space having a substantially constant magnetic force field is also provided.
摘要翻译： 本发明提供了用于确定多个粒子物理特性中的至少一个的方法和装置。 颗粒物理特性包括粒度，形状，磁化率，磁性标记密度，电荷分离，介电常数及其衍生物。 该方法包括产生具有基本上恒定的力场的空间区域，通过在至少两个时间上定义的数字图像中识别和定位粒子及其坐标来确定该区域内的至少一个粒子的速度，以及确定粒子物理特性 从确定的速度和预定的力场大小和方向。 描述了一种用于确定一个或多个粒子物理特性的装置，其具有用于使至少一个粒子经受至少一个力场的力场装置，基本上透明的流动通道，以及用于收集和分析与该相关联的数据的数据的计算机系统 至少一个粒子。 提供了一种用于确定一个或多个粒子物理特性的系统，其具有用于产生具有预定力场大小和方向的至少一个力场并且用于使至少一个粒子经受至少一个力场的力场装置， 用于调节将至少一个粒子引入力场设备的系统，以及用于收集和分析与至少一个粒子相关联的数据的计算机系统。 还提供了一种用于产生具有基本上恒定的磁力场的空间区域的极片组件。
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公开(公告)号：US6142025A
公开(公告)日：2000-11-07
申请号：US232956
申请日：1999-01-19
IPC分类号： G01N15/10 , G01N15/14 , G01N27/447 , G01P5/18 , G01N21/00
CPC分类号： G01N15/10 , G01N27/447 , G01P5/18 , G01N2015/1075 , G01N2015/1081 , G01N2015/149
摘要： The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing a region of space having a substantially constant magnetic force field is also provided.
摘要翻译： 本发明提供了用于确定多个粒子物理特性中的至少一个的方法和装置。 颗粒物理特性包括粒度，形状，磁化率，磁性标记密度，电荷分离，介电常数及其衍生物。 该方法包括产生具有基本上恒定的力场的空间区域，通过在至少两个时间上定义的数字图像中识别和定位粒子及其坐标来确定该区域内的至少一个粒子的速度，以及确定粒子物理特性 从确定的速度和预定的力场大小和方向。 描述了一种用于确定一个或多个粒子物理特性的装置，其具有用于使至少一个粒子经受至少一个力场的力场装置，基本上透明的流动通道，以及用于收集和分析与该相关联的数据的数据的计算机系统 至少一个粒子。 提供了一种用于确定一个或多个粒子物理特性的系统，其具有用于产生具有预定力场大小和方向的至少一个力场并且用于使至少一个粒子经受至少一个力场的力场装置， 用于调节将至少一个粒子引入力场设备的系统，以及用于收集和分析与至少一个粒子相关联的数据的计算机系统。 还提供了一种用于产生具有基本上恒定的磁力场的空间区域的极片组件。
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公开(公告)号：US5974901A
公开(公告)日：1999-11-02
申请号：US20330
申请日：1998-02-06
IPC分类号： G01N15/10 , G01N15/14 , G01N27/447 , G01P5/18 , G01N21/00
CPC分类号： G01N15/10 , G01N27/447 , G01P5/18 , G01N2015/1075 , G01N2015/1081 , G01N2015/149
摘要： The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing a region of space having a substantially constant magnetic force field is also provided.
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公开(公告)号：US20050079600A1
公开(公告)日：2005-04-14
申请号：US10626221
申请日：2003-07-24
申请人： Aaron Fleischman , Shuvo Roy , Jeff Chalmers , Maciej Zborowski
发明人： Aaron Fleischman , Shuvo Roy , Jeff Chalmers , Maciej Zborowski
IPC分类号： B81B1/00 , G01N15/10 , G01N15/14 , G01N33/543 , C12M1/34 , G01N33/553
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/043 , B01L2400/0487 , C12M47/04 , G01N15/1031 , G01N15/1056 , G01N15/1456 , G01N2015/1006 , G01N2015/1062 , G01N2015/1081
摘要： The present invention provides a MEMS-based integrated particle identification system having a substrate, a magnetic structure, and a bioferrograph. The substrate includes a topside portion, backside portion and a flow system. The flow system includes a flow channel for accepting the flow of a stream of particles to identified. The magnetic structure is in physical communication with the topside and backside portions of the substrate and has at least two pole pieces. A plurality of pole piece embodiments are provided for generating a magnetic field that acts on magnetically susceptible particles in the flow stream. The bioferrograph has at least one sensor for identifying the presence and quantity of magnetically susceptible particles. A plurality of sensor embodiments are also provided.
摘要翻译： 本发明提供了一种基于MEMS的集成粒子识别系统，该系统具有基底，磁性结构和生物计量学。 衬底包括顶侧部分，后侧部分和流动系统。 流动系统包括用于接收所识别的颗粒物流的流动通道。 磁性结构与衬底的顶侧和后侧部分物理连通，并具有至少两个极片。 提供了多个极片实施例，用于产生作用在流动流中的易磁敏颗粒上的磁场。 生物传感器具有至少一个用于识别磁敏感颗粒的存在和数量的传感器。 还提供了多个传感器实施例。
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