公开(公告)号：US06509443B1

公开(公告)日：2003-01-21

申请号：US09723896

申请日：2000-11-28

申请人： Yves Dubaquie ,  Henry Lowman

发明人： Yves Dubaquie ,  Henry Lowman

IPC分类号： C07K1465

CPC分类号： C07K14/65 ,  A61K38/00

摘要： IGF-I and insulin variants are provided that selectively bind to IGFBP-1 or IGFBP-3. These agonist variants are useful, for example, to improve the half-lives of IGF-I and insulin, respectively.

摘要翻译： 提供了选择性结合IGFBP-1或IGFBP-3的IGF-1和胰岛素变体。 这些激动剂变体可用于分别改善IGF-1和胰岛素的半衰期。

公开(公告)号：US06331609B1

公开(公告)日：2001-12-18

申请号：US08464361

申请日：1995-06-05

申请人： James M. Lee ,  Axel Ullrich ,  Arjun Singh

发明人： James M. Lee ,  Axel Ullrich ,  Arjun Singh

IPC分类号： C07K1465

CPC分类号： C12N9/2408 ,  A61K38/00 ,  C07K14/485 ,  C07K14/65 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/75 ,  C12N15/81

摘要： Human insulin-like growth factor is synthesized in recombinant cell culture by host cells transformed with expression vectors bearing DNA encoding human insulin-like growth factor.

摘要翻译： 通过用编码人类胰岛素样生长因子的DNA的表达载体转化的宿主细胞在重组细胞培养物中合成人胰岛素样生长因子。

公开(公告)号：US06518238B1

公开(公告)日：2003-02-11

申请号：US09418861

申请日：1999-10-15

申请人： Desmond Mascarenhas

发明人： Desmond Mascarenhas

IPC分类号： C07K1465

CPC分类号： A61K38/30 ,  Y10S514/866 ,  A61K2300/00

摘要： Methods are provided for treating or alleviating the symptoms of subjects with psychological disorders, metabolic disorders, chronic stress-related disorders, sleep disorders, conditions associated with sexual senescence, aging, or premature aging by treating such subjects with IGF or mutant IGF either alone or complexed with IGFBP-3. Methods for increasing the levels of DHEA or DHEAS and treating or alleviating the symptoms of subjects with disorders characterized by low levels of DHEA or DHEAS by administering effective amounts of IGF or mutant IGF alone or complexed with IGFBP-3 are also provided. Methods for increasing the level of T4 and treating or alleviating the symptoms of subjects with disorders characterized by low levels of T3 or T4 by administering effective amounts of IGF or mutant IGF alone or complexed with IGFBP-3 are additionally provided.

摘要翻译： 提供了用于治疗或减轻患有心理障碍，代谢紊乱，慢性应激相关疾病，睡眠障碍，与性衰老相关的病症，衰老或过早衰老的受试者的症状的方法，其通过用IGF或突变IGF单独或突变IGF 与IGFBP-3复合。 还提供了通过单独施用有效量的IGF或突变型IGF或与IGFBP-3复合来提高DHEA或DHEAS水平和治疗或减轻以低水平DHEA或DHEAS为特征的疾病的受试者症状的方法。 另外提供了通过施用有效量的IGF或突变IGF单独或与IGFBP-3复合来增加T4水平并治疗或减轻以T3或T4低水平为特征的受试者症状的方法。

公开(公告)号：US06235874B1

公开(公告)日：2001-05-22

申请号：US09383212

申请日：1999-08-26

申请人： Jen-Leih Wu ,  Jyh-Yih Chen

发明人： Jen-Leih Wu ,  Jyh-Yih Chen

IPC分类号： C07K1465

CPC分类号： C07K14/65

摘要： The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH2—B—C—A—D—COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II cDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells. The isolated and purified IGF-II E domain peptide has mitogenic and anti-tumor activity.

摘要翻译： 本发明涉及可以在细胞中克隆和表达的鱼类胰岛素样生长因子II（IGF-II）cDNA的发现和构建。 本发明还涉及通过使用鱼IGF-II cDNA的基因表达系统生产生物活性鱼IGF-II多肽。 鱼IGF-II cDNA具有1977bp，其转录成前肽（信号肽），以及B，C，A，D，E结构域肽。 鱼成熟IGF-II是含有NH2-B-C-A-D-COOH结构域的单一多肽。 成熟的IGF-II多肽的重量为7kDa，具有70个氨基酸。 鱼重组IGF-II cDNA可以克隆并表达在大肠杆菌，酵母，杆状病毒和鱼细胞中。 分离和纯化的IGF-II E结构域肽具有促有丝分裂和抗肿瘤活性。