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6 条记录 (耗时 0.01 秒)

    Heterologous expression of cellular adhesion molecules
    2.
    发明授权
    Heterologous expression of cellular adhesion molecules 有权
    细胞粘附分子的异源表达

    公开(公告)号：US09273090B1

    公开(公告)日：2016-03-01

    申请号：US13408722

    申请日：2012-02-29

    申请人： Stephen K. Herbert ,  Levi G. Lowder

    发明人： Stephen K. Herbert ,  Levi G. Lowder

    IPC分类号： C12N1/13 ,  C07K4/08

    CPC分类号： C07K4/08 ,  C12N1/02 ,  C12N1/12 ,  C12N15/8257

    摘要： Methods for the production of unicellular photosynthetic organisms capable of producing cell adhesion proteins are disclosed. DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular photosynthetic organisms for the expression of cell adhesion proteins are also disclosed.

    摘要翻译： 公开了生产能够产生细胞粘附蛋白的单细胞光合生物的方法。 还公开了DNA构建体以及用于将DNA构建体整合到单细胞光合生物的基因组中以用于表达细胞粘附蛋白的方法。

    Selective heated extraction of globulin proteins from intact freshwater algal cells
    3.
    发明授权
    Selective heated extraction of globulin proteins from intact freshwater algal cells 失效
    从完整的淡水藻细胞中选择性加热提取球蛋白

    公开(公告)号：US08741629B2

    公开(公告)日：2014-06-03

    申请号：US13289801

    申请日：2011-11-04

    申请人： Aniket Kale

    发明人： Aniket Kale

    IPC分类号： C12N1/12 ,  C07K4/08 ,  A61K38/00

    CPC分类号： C11B1/10 ,  B01D11/04 ,  C11B1/108 ,  Y02E50/13

    摘要： Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

    摘要翻译： 公开了从藻类生物质或藻类培养物中选择性提取和分离藻类蛋白的方法。 从藻类生物质中选择性地除去产物的方法提供允许有效分离藻类蛋白质的单步骤和多步萃取方法。 这些蛋白质可用作动物原料和人类食物的可再生来源蛋白质。 此外，在提取蛋白质之后残留在藻类生物质中的脂质可用于产生可再生燃料。

    Aerosol beam microinjector
    4.
    发明授权
    Aerosol beam microinjector 失效
    气溶胶束微量注射器

    公开(公告)号：US5240842A

    公开(公告)日：1993-08-31

    申请号：US902478

    申请日：1992-06-19

    申请人： Laurens J. Mets

    发明人： Laurens J. Mets

    IPC分类号： C07K4/08 ,  C12M3/00 ,  C12N15/87

    CPC分类号： C12N15/87 ,  C12M35/00 ,  C12M35/04

    摘要： The present invention uses aerosol beam technology to accelerate either wet or dry aerosol particles to speeds enabling the particles to penetrate living cells. Aerosol particles suspended in an inert gas are accelerated to a very high velocity during the jet expansion of the gas as it passes from a region of higher gas pressure to a region of lower gas pressure through a small orifice. The accelerated particles are positioned to impact a preferred target, for example, a plant or animal cell or bacterial culture. When the droplets include DNA or other macromolecules, the macromolecules are introduced into the cells. The particles are constructed as droplets of a sufficiently small size so that the cells survive the penetration. Once introduced into the target cell the macromolecules can elicit biological effects. Because the method of introduction is a physical one, the biological barriers that restrict the application of other DNA transfer methods to a few plant species and a few cell types are not present. In addition, the method and apparatus of the present invention permit the treatment of a large number of cells in the course of any single treatment. Thus, the inventive method and apparatus should be applicable to a wide range of plant species and cell types that have proved in the past to be quite impervious to standard methods of genetic engineering.

    摘要翻译： 本发明使用气溶胶束技术来加速湿或干燥的气溶胶颗粒，以加速颗粒穿透活细胞。 悬浮在惰性气体中的气溶胶颗粒在气体从较高气体压力的区域通过小孔口流过较低气体压力的区域时，在气体的射流膨胀过程中被加速至非常高的速度。 加速的颗粒定位成影响优选的靶，例如植物或动物细胞或细菌培养物。 当液滴包含DNA或其他大分子时，将大分子引入细胞。 颗粒被构造成足够小的液滴，使得细胞在穿透中存活。 一旦引入靶细胞，大分子可以引发生物学效应。 由于引入方法是物理方法，因此不存在将其他DNA转移方法应用于少数植物物种和少数细胞类型的生物学障碍。 此外，本发明的方法和装置允许在任何单一处理过程中处理大量的细胞。 因此，本发明的方法和装置应当适用于过去证明的广泛的植物物种和细胞类型对基因工程的标准方法是非常不可渗透的。