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公开(公告)号：US3972775A
公开(公告)日：1976-08-03
申请号：US484010
申请日：1974-06-28
申请人： Charles R. Wilke , Gautam Mitra
发明人： Charles R. Wilke , Gautam Mitra
CPC分类号： C12N9/2437 , C12P19/14 , C12Y302/01004 , C13K1/02 , Y10S435/813 , Y10S435/816 , Y10S435/929 , Y10S435/945
摘要： A process for the production of sugar, mainly glucose, by the enzymatic degradation of cellulosic materials, particularly cellulosic wastes, which comprises hydrolyzing the cellulosic material in the presence of cellulase enzyme to produce a sugar solution and recovering from the hydrolysis products a major proportion of the cellulase enzyme used in the hydrolysis reaction for re-use. At least a portion of the required makeup cellulase enzyme is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is utilized to grow a cellulase-secreting microorganism, and, in the second stage, cellulase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer, such as a cellulosic material. Cellulase enzyme is precipitated from the culture liquid by the addition of an organic solvent material, such as a low molecular weight alkyl ketone or alcohol, and the cellulase precipitate is then fed to the hydrolysis reaction.
摘要翻译： 一种通过纤维素材料，特别是纤维素废物的酶降解来生产糖，主要是葡萄糖的方法，其包括在纤维素酶存在下水解纤维素材料以产生糖溶液并从水解产物中回收大部分 用于水解反应中的纤维素酶用于再利用。 至少一部分所需的补充纤维素酶在两阶段操作中产生，其中在第一阶段中，将一部分输出的糖溶液用于生长纤维素酶分泌微生物，并且在第二阶段中，纤维素酶 通过添加合适的诱导剂如纤维素材料在含微生物的培养基中诱导酶的形成。 纤维素酶通过加入有机溶剂材料如低分子量烷基酮或醇从培养液中沉淀出来，然后将纤维素酶沉淀物加入到水解反应中。
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公开(公告)号：US3926723A
公开(公告)日：1975-12-16
申请号：US49560974
申请日：1974-08-08
发明人： GREEN HOWARD , RHEINWALD JAMES G
CPC分类号： C12N5/0018 , C12N2500/34
摘要： A cell culture medium is disclosed which contains secondary sources of glucose comprising glucose-containing oligosaccharides or polysaccharides and hydrolytic enzymes (glucosidases) to liberate free glucose. Starch, for example, can be added to a culture medium to provide the controlled release of glucose when a serum supplement such as fetal calf serum, which contains the hydrolytic enzymes amylase and maltase, is used. Amylase breaks the starch down into glucose and maltose, and maltase converts the remaining maltose to glucose. The rate of glucose release can be controlled by regulating the amount of glucosidase activity present in the medium.
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