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1.发明授权
公开(公告)号：US4150150A
公开(公告)日：1979-04-17
申请号：US835922
申请日：1977-09-23
申请人： Otto C. Straub
发明人： Otto C. Straub
CPC分类号： A61K39/0011 , A61K2039/5152
摘要： A method has been developed for the modification of tumour cells by treatment of said tumour cells in aqueous suspension with an alternating electric current so that said tumour cells lose their pathogenic activity and carcinogenic activity and still possess only antigenic activity, stimulating the immunological system against themselves. In addition to the above-described method, the invention also includes the carcinostatically active material obtained by the above-described process, pharmaceutical compositions containing said carcinostatically active material and methods for the treatment of tumours.
摘要翻译： 已经开发了一种通过用交替电流处理水悬浮液中的所述肿瘤细胞来修饰肿瘤细胞的方法，使得所述肿瘤细胞失去其致病活性和致癌活性，并且仍然仅具有抗原活性，刺激免疫系统对抗自身 。 除了上述方法之外，本发明还包括通过上述方法获得的制癌活性物质，含有所述制癌活性物质的药物组合物和治疗肿瘤的方法。
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2.发明授权Viral oncolysate vaccine for stimulating the immune mechanism of mammals to species-specific tumors 失效用于刺激哺乳动物对特异性肿瘤的免疫机制的病毒性溶解疫苗
公开(公告)号：US4108983A
公开(公告)日：1978-08-22
申请号：US691486
申请日：1976-06-01
申请人： Marc K. Wallack
发明人： Marc K. Wallack
IPC分类号： C12N7/00 , A61K35/12 , A61K35/76 , A61K38/00 , A61K39/00 , A61K39/12 , A61K39/285 , A61K39/295 , C12K9/00
CPC分类号： A61K39/0011
摘要： A virus-lysed tumor cell vaccine is an active immunotherapeutic agent against tumors in mammals. In particular, a vaccine based upon vaccinia virus-lysed, species-specific tumor cells is an effective stimulator of the immune response in mammals, and a vaccine based upon vaccinia virus-lysed spontaneously arising tumor cells is an effective stimulator of the immune response in some human cancer patients. A process for preparing the vaccine by viral oncolysis is also disclosed. Tumor cells are removed from a mammal, the cells are cultured in a culture medium and infected with live vaccinia virus. Viral oncolysis occurs during incubation and the resulting viral oncolysate, after extraction, may be injected into mammals to stimulate the immune response mechanism.
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公开(公告)号：US4085006A
公开(公告)日：1978-04-18
申请号：US521518
申请日：1974-11-06
申请人： Leo R. Mindick , Avraham Bruck , Dan Inbar , Leopold G. Koss
发明人： Leo R. Mindick , Avraham Bruck , Dan Inbar , Leopold G. Koss
IPC分类号： G01N33/483 , C12K1/00 , C12K9/00
CPC分类号： G01N33/5005 , G01N33/574
摘要： A cell analyzer which automatically processes and evaluates cell samples for possible indications of malignancy and other abnormalities is disclosed. Cell samples are prepared in a suspension and are tagged with an appropriate radioactive stain or appropriate biochemical. Counting, filtering and drying steps under computer control are followed by the taking of a radioactive count with respect to a normalized number of cells. The results of the radioactive counting determine whether the particular sample is classified as "positive" or "negative".
摘要翻译： 公开了一种细胞分析仪，其自动处理和评估细胞样品以获得恶性肿瘤和其他异常的可能指征。 细胞样品以悬浮液制备，并用适当的放射性染色剂或适当的生化标记。 计算机控制下的计数，过滤和干燥步骤之后是对标准化数量的细胞进行放射性计数。 放射性计数的结果确定特定样品是否被归类为“阳性”或“阴性”。
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公开(公告)号：US4073695A
公开(公告)日：1978-02-14
申请号：US713441
申请日：1976-08-11
申请人： George F. Lyman
发明人： George F. Lyman
CPC分类号： C12M23/08
摘要： A Leighton tube assembly designed for the growth of tissue cultures and their preservation comprising a tube, a cover slip and a means to close said tube. The tube, made from two injection molded pieces welded together, has a chamber toward its closed end and a neck towards its open end. The chamber has a wall with a flat portion which is separated from the open end of the tube by a dam located in the neck. The cover slip is molded of a plastic material which is both transparent and inert with respect to most chemical solvents. The cover slip has a culture segment which lies on said flat portion of the chamber wall when the slip is in its growth position and a handle. The handle is of a length which allows the positioning of a cap over the open end of the tube.
摘要翻译： Leighton管组件设计用于组织培养物的生长及其保存，其包括管，盖板和封闭所述管的装置。 由两个注射成型件焊接在一起的管具有朝向其封闭端的腔室和朝向其开口端的颈部。 该室具有一个具有平坦部分的壁，其通过位于颈部中的坝与管的开口端分离。 盖板由塑料材料成型，对于大多数化学溶剂而言，它是透明的和惰性的。 当滑移处于其成长位置时，盖板具有位于室壁的所述平坦部分上的培养段和手柄。 手柄的长度允许将盖定位在管的开口端上。
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公开(公告)号：US4064007A
公开(公告)日：1977-12-20
申请号：US663279
申请日：1976-03-02
申请人： Jean Choay , Paul Trolard , Henri Lucien Febvre
发明人： Jean Choay , Paul Trolard , Henri Lucien Febvre
CPC分类号： A61K35/15
摘要： Continuous cell lines of macrophages are provided by the in vitro action, on mammalian macrophages withdrawn in situ, of macrophage activating substances, which cause the removal by the macrophages of the other cells present in the cellular medium withdrawn. The macrophages thus isolated are then placed in a suitable culture medium, until the establishment of a continuous line of macrophages, then the macrophages established as a continuous line are cultured in the presence of a suitable activating substance and the supernatant liquid is collected from these cultures. This supernatant contains substances inhibiting the proliferation of the cells having a rapid rate of multiplication. This supernatant liquid is, if necessary, subjected to a purification process. The product is notably useful for inhibiting human tumor cells.
摘要翻译： 巨噬细胞的连续细胞系通过体外作用，原位取出的巨噬细胞活化物质的哺乳动物巨噬细胞提供，其引起细胞介质中存在的其他细胞的巨噬细胞的去除。 然后将如此分离的巨噬细胞置于合适的培养基中，直到建立连续的巨噬细胞系，然后在合适的活化物质存在下培养建立为连续线的巨噬细胞，并从这些培养物中收集上清液 。 该上清液含有抑制具有快速繁殖速率的细胞增殖的物质。 如果需要，该上清液进行纯化处理。 该产品对于抑制人肿瘤细胞特别有用。
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公开(公告)号：US4028190A
公开(公告)日：1977-06-07
申请号：US632610
申请日：1975-11-17
摘要： An apparatus and a method for preparing live, sterile cells from tissue are disclosed which enable high yields of viable isolated cells to be obtained with a greatly reduced possibility of contamination of the cells during the process. The apparatus and method allows one to truly mass manufacture isolated cells as contrasted to a small hand operation requiring many hours and dozens of people. The improved apparatus of the invention comprises a shredder device which mechanically shreds the tissue into small pieces of high surface to volume ratio, a processing vessel in which the shredded tissues are contacted with an enzyme solution to break down the intercellular material and thereby separate the cells one from the other and a means for separating the cells from the enzyme solution. Preferably, the means for separating the cells from the enzyme solution is a flow centrifuge which has been specifically designed to reduce shear force inside the bowl during operation. Also disclosed is a process for shredding tissue employing a plurality of moveable blade elements and at least a corresponding number of separated fixed elements. Interposing the tissue between the fixed and rotating elements and then driving the moveable blade elements through the spaces defined by adjacent fixed elements results in the tissue being shredded into small pieces of high surface to volume ratio. This in turn optimizes surface area for contact between the shredded tissue and the enzyme solution. In addition, a completely closed process is disclosed for obtaining cells from the tissue comprising the steps of sequentially shredding the tissue, contacting the shredded tissue at a controlled temperature with an enzyme solution to break down the intercellular material and then separating the isolated cells from the enzyme solution.
摘要翻译： 公开了一种用于从组织制备活的无菌细胞的装置和方法，其使得可以在该过程中大大降低细胞污染的可能性而获得高得多的活的分离的细胞。 该装置和方法允许真正大量制造隔离的细胞，与需要数十小时和数十人的小手操作相反。 本发明的改进的装置包括一个粉碎装置，其将组织机械地切碎成高的表面与体积比的小块;处理容器，其中切碎的组织与酶溶液接触以分解细胞间材料，从而分离细胞 一个来自另一个和用于从酶溶液中分离细胞的方法。 优选地，用于从酶溶液中分离细胞的装置是流动离心机，其已经被专门设计用于在操作期间减小碗内的剪切力。 还公开了一种使用多个可移动叶片元件和至少相应数量的分离的固定元件来切碎组织的方法。 将组织插入固定和旋转元件之间，然后通过由相邻的固定元件限定的空间驱动可移动的刀片元件，导致组织被切碎成高的表面与体积比的小块。 这又优化了切碎的组织和酶溶液之间的接触的表面积。 另外，公开了一种从组织获得细胞的完全封闭的方法，包括以下步骤：顺序地切碎组织，在受控温度下将切碎的组织与酶溶液接触以分解细胞间材料，然后将分离的细胞从 酶溶液。
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公开(公告)号：US4018649A
公开(公告)日：1977-04-19
申请号：US623238
申请日：1975-10-17
CPC分类号： C12N5/00 , C12N2500/10 , C12N2500/12
摘要： A method of controlling mitosis of biological cells by inducing a change in the intracellular ionic hierarchy accompanying the cellular electrical transmembrane potential difference (E.sub.m) of the cells. The ionic hierarchy may be varied by imposing changes on the relative concentrations of Na.sup.+, K.sup.+, and Cl.sup.-, or by directly imposed changes of the physical E.sub.m level across the cell surface.
摘要翻译： 通过诱导伴随细胞的细胞电跨膜电位差（Em）的细胞内离子层级的变化来控制生物细胞有丝分裂的方法。 可以通过对Na +，K +和Cl - 的相对浓度施加变化，或通过直接施加的跨越细胞表面的物理Em水平的变化来改变离子层级。
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公开(公告)号：US4003789A
公开(公告)日：1977-01-18
申请号：US539378
申请日：1975-01-08
申请人： Howard Green
发明人： Howard Green
CPC分类号： C12N5/0656 , C12N2503/02
摘要： An isolated clonal cell line derived from the mouse fibroblast 3T3 line is disclosed. This 3T3-L1 clonal cell line has the unique characteristic of accumulating large amounts of triglyceride fats in its resting state, which makes it useful for screening drugs to determine their effect on cell fat accumulation.
摘要翻译： 公开了从小鼠成纤维细胞3T3系衍生的分离的克隆细胞系。 该3T3-L1克隆细胞系具有在其静息状态下积累大量甘油三酯脂肪的独特特征，使其可用于筛选药物以确定其对细胞脂肪蓄积的影响。
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9.发明授权
公开(公告)号：US4001428A
公开(公告)日：1977-01-04
申请号：US598133
申请日：1975-07-22
IPC分类号： C07C53/134 , A61K31/25 , C07C69/00 , C12K9/00
CPC分类号： C07C53/134
摘要： Compounds capable of functioning as membrane mobility agents in plant and animal cells are described. These compounds have a structure represented by the formula ##STR1## in which A represents a hydrophilic chain; Z represents either an ester linkage ##STR2## an amide linkage ##STR3## or an ether linkage (--O--); R.sub.1 represents either a straight-chain alkyl group or a straight-chain alkyl group interrupted by one or more cyclopropyl groups; R.sub.2 and R.sub.3 each represent either hydrogen, lower alkyl, or a halogen group; and x represents an integer from 2 to 10.The membrane mobility agents of the invention promote the lateral mobility through cell membranes of molecules ranging in size from phospholipids to surface antigens. They represent a new class of biologically active molecules which may be used to alter the mobility of membrane components in animal and plant cells to alter such cells in their response to external stimuli. Among their uses are altering the immunological response of lymphocytes; altering the pharmacological response of target cells; altering the biological properties of cancer cells; and fusing two or more cells to create a new hybrid cell containing the genetic information from the fused cells.
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公开(公告)号：US3973003A
公开(公告)日：1976-08-03
申请号：US533382
申请日：1974-12-16
申请人： Guy Colas
发明人： Guy Colas
CPC分类号： A61K35/52 , A01N1/021 , A01N1/0221 , A01N1/0284 , A61D19/00
摘要： The process according to the invention consists in that the freshly collected ram semen is first diluted at between 25.degree. and 32.degree.C and notably at 30.degree.C, in a diluent without glycerol containing lactose and egg yolk, and in then diluting, at between 3.degree. and 5.degree.C and notably at 4.degree.C, the mixture previously obtained in a diluent containing glycerol, said diluent essentially comprising powdered milk and sodium citrate, in conditioning in straws the so diluted semen and in freezing it in liquid nitrogen at about -75.degree.C. The invention also relates to the diluent used in the above process.The process is useful for preserving ram semen by freezing for subsequent artificial insemination of ewes.
摘要翻译： 根据本发明的方法在于，新鲜收集的乳清精液首先在25℃至32℃，特别是在30℃下稀释，不含甘油的乳糖和蛋黄的稀释剂中，然后在两者之间稀释 3℃和5℃，特别是在4℃，预先在含有甘油的稀释剂中获得的混合物，所述稀释剂基本上包含奶粉和柠檬酸钠，在吸管中调理如此稀释的精液，并将其冷冻在液氮中约 -75℃。本发明还涉及上述方法中使用的稀释剂。
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