公开(公告)号：US06787349B1

公开(公告)日：2004-09-07

申请号：US09889747

申请日：2001-07-19

申请人： Kenji Yamamoto ,  Mitsuhiro Tachibana ,  Katsuya Mizuno

发明人： Kenji Yamamoto ,  Mitsuhiro Tachibana ,  Katsuya Mizuno

IPC分类号： C12M100

CPC分类号： G01N33/543 ,  B01J2219/00536 ,  Y10T436/13

摘要： The present invention provides a high-sensitive, inexpensive biochip reader for reading a biochip as compared to a fluorescence method. The biochip reader is provided with an X-Y stage (3) for mounting a biochip (6) and scanning the biochip (6) in a two-dimensional manner, a controller (4) for the X-Y stage, a magnetic sensor (1) for reading the magnetic field strength, an ohmmeter (2), and a computer (5) for signal processing. As a result, a high-performance, inexpensive biochip reader can be provided without using an expensive laser or an expensive optical system, by employing a magnetic sensor and a disk driving mechanism generally used in a hard disk drive and the like.

摘要翻译： 与荧光方法相比，本发明提供了一种用于读取生物芯片的高灵敏度，便宜的生物芯片读取器。 生物芯片读取器设置有用于安装生物芯片（6）并以二维方式扫描生物芯片（6）的XY台（3），用于XY台的控制器（4），用于 读取磁场强度，欧姆表（2）和用于信号处理的计算机（5）。 结果，通过采用通常用于硬盘驱动器等中的磁传感器和盘驱动机构，可以提供高性能，廉价的生物芯片读取器，而不需要使用昂贵的激光器或昂贵的光学系统。

公开(公告)号：US06783974B2

公开(公告)日：2004-08-31

申请号：US09901351

申请日：2001-07-09

申请人： Beth C. Monahan ,  Everett W. Coonan

发明人： Beth C. Monahan ,  Everett W. Coonan

IPC分类号： C12M100

CPC分类号： B01L3/50851

摘要： A microplate is described that has a surface with an enhanced lubricious property which makes it easier to remove the microplate from a thermocycler. Basically, the microplate has a frame which includes an array of wells formed therein that are made from a thermoplastic material (e.g. polypropylene) mixed with a non-toxic surface active material (e.g., surfactant, stearyl alcohol). The non-toxic surface active material functions to enhance the lubricity of the surface of the microplate which makes it easier to remove the microplate from the thermocycler. In addition, the non-toxic surface active material within the microplate also makes it easier to remove a newly molded microplate from a mold cavity in an injection molding machine. Also described herein are details about methods for making and using such microplates.

摘要翻译： 描述了具有增强的润滑性质的表面的微孔板，其使得更容易从热循环仪中除去微孔板。 基本上，微孔板具有框架，其包括由其中与无毒表面活性材料（例如表面活性剂，硬脂醇）混合的热塑性材料（例如聚丙烯）制成的孔阵列。 无毒的表面活性物质用于增强微孔板表面的润滑性，从而更容易从热循环仪中除去微孔板。 此外，微量培养板内的无毒表面活性物质也使得更容易从注射成型机中的模腔中移除新模制的微孔板。 本文还描述了关于制造和使用这种微孔板的方法的细节。

公开(公告)号：US06767734B2

公开(公告)日：2004-07-27

申请号：US10082906

申请日：2002-02-25

申请人： Shi V. Liu

发明人： Shi V. Liu

IPC分类号： C12M100

CPC分类号： C12M25/02 ,  C12M23/20 ,  C12M29/12

摘要： The present invention relates to a method and an apparatus for producing cell population of the same chronological age. Cells of a predetermined age are collected onto an array of surfaces. The collected cells are retained on the said surface during their continuous cultivation and kept from mixing with their descendants by means of flushing away their offspring. Sampling of surface from the array at desired time gives cell populations of desired cell age for examination, assay and other different uses.

摘要翻译： 本发明涉及一种具有相同年龄的细胞群的生产方法和装置。 将预定年龄的细胞收集到表面阵列上。 收集的细胞在其连续培养期间保留在所述表面上，并通过冲洗其后代而与其后代保持混合。 在期望的时间从阵列取样表面，给予细胞群体所需的细胞年龄用于检查，测定和其他不同用途。

公开(公告)号：US06730883B2

公开(公告)日：2004-05-04

申请号：US10262994

申请日：2002-10-02

申请人： Larry R. Brown ,  William D. Brumley ,  Kenneth J. Zajac

发明人： Larry R. Brown ,  William D. Brumley ,  Kenneth J. Zajac

IPC分类号： C12M100

CPC分类号： B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L7/54 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844

摘要： A flexible heating cover assembly for an apparatus for heating samples of biological material with substantial temperature uniformity includes a housing having a plurality of engageable enclosure components; a resistive heater having a plurality of heater element areas; a heater backing plate providing stability to the resistive heater; a force distribution system that distributes a force over the heater backing plate; and a support plate providing stiffness for the force distribution system, wherein the arrangement of the resistive heater, the heater backing plate, the force distribution system and the support plate provide substantial temperature uniformity among a plurality of sample tubes for receiving samples of biological material. The flexible heating cover assembly improves the uniformity, efficiency, quality, reliability and controllability of the thermal response during thermal cycling of the biological material.

摘要翻译： 一种用于加热具有显着温度均匀性的生物材料样品的装置的柔性加热盖组件包括具有多个可接合外壳部件的壳体; 电阻加热器，具有多个加热元件区域; 加热器背板，为电阻加热器提供稳定性; 力分配系统，其在加热器背板上分布力; 以及为力分配系统提供刚度的支撑板，其中电阻加热器，加热器背板，力分配系统和支撑板的布置在用于接收生物材料样品的多个样品管之间提供显着的温度均匀性。 柔性加热盖组件改善了生物材料热循环期间的热响应的均匀性，效率，质量，可靠性和可控性。

公开(公告)号：US06673592B1

公开(公告)日：2004-01-06

申请号：US08734184

申请日：1996-10-21

申请人： Jaw-Kai Wang ,  Tim Hering

发明人： Jaw-Kai Wang ,  Tim Hering

IPC分类号： C12M100

CPC分类号： C12M21/02 ,  A01G33/00 ,  C12M23/08 ,  C12M41/32 ,  C12N1/12

摘要： An open, continuous system for culturing Chaetoceros sp. microalgae includes a large outdoor container and full strength sunlight as a light source. The container is preferably a fiberglass tank having an open top, a diameter of approximately 18 inches, and a height of about 5 feet. The container holds a culture medium having the following characteristics: a carbon dioxide controlled pH of about 8.2, a starting nitrogen concentration of at least 3.0 mg N/liter, a starting phosphorous concentration of at least 2.75 mg P/liter, a starting vitamin B12 concentration of at least 5 micrograms/liter, a starting iron chloride concentration of at least 0.3 mg/liter, a starting copper sulfate concentration of at least 0.01 mg/liter, a starting silicate concentration of at least 10 mg SiO2/liter, and a Na2EDTA concentration of 5 mg/liter. The medium is inoculated with a seed stock of Chaetoceros sp. microalgae and exposed direct sunlight. A portion of the cultured microalgae, preferably in the 90% range, is harvested each day and replaced with new culture media. As sterile conditions are not required, seawater may be used as the replacing culture media.

摘要翻译： 一种开放，连续的培养Chaetoceros sp。 微藻包括大型户外集装箱和充分强度的阳光作为光源。 容器优选为具有开口顶部，直径约18英寸，高度约5英尺的玻璃纤维槽。 容器装有具有以下特征的培养基：二氧化碳控制pH约8.2，起始氮浓度至少为3.0mg N /升，起始磷浓度至少为2.75mg P /升，起始维生素B12 浓度为至少5微克/升，起始氯化铁浓度至少为0.3mg /升，起始硫酸铜浓度为至少0.01mg /升，起始硅酸盐浓度为至少10mg /升，和 Na2EDTA浓度为5 mg / l。 用Chaetoceros sp。的种子种子接种培养基。 微藻和暴露的阳光直射。 每天收获一部分培养的微藻，优选在90％范围内，并用新的培养基替代。 由于不需要无菌条件，可以使用海水作为替代培养基。

公开(公告)号：US06579713B2

公开(公告)日：2003-06-17

申请号：US10094488

申请日：2002-03-08

申请人： Paul A. Olivier

发明人： Paul A. Olivier

IPC分类号： C12M100

CPC分类号： C05F17/0009 ,  C05F17/0247 ,  Y02P20/145 ,  Y02W30/43

摘要： Initially, putrescent waste is deposited on a surface of a disposal volume which is partially composed of putrescent waste. A living system bio-converts at least a portion of the putrescent waste in the disposal volume, transforming the putrescent waste into waste residue. While bio-conversion is occurring on a portion of the putrescent waste in the disposal volume, waste residue is excavated from the disposal volume below the surface of the disposal volume. Because the excavation takes place below the surface area, the bio-conversion process is not affected by excavating the waste residue. A device for continuous bio-conversion of putrescent waste comprises a disposal track having lateral side walls and floor for containing the disposal volume. An excavation gap is associated with at least one of the lateral side walls for excavating waste material from the disposal volume. The excavation gap is positioned substantially below the living system so waste can be excavated simultaneously with bio-conversion. The device further employs a scraper for excavating at least a portion of the waste residue. The scraper has a plurality of blades attached to a chain for excavating the waste and interposed between the blades are backplates which clean the blades as the chain rounds a sprocket. The scraper is moved along the floor of the disposal track as it excavates the waste material. The floor both supports the disposal volume and the scraper, and has a filter screen to filter water from the disposal volume.

摘要翻译： 最初，将粉末状废物沉积在部分由粉末废物组成的处理容积的表面上。 一个生物系统将处置量中至少一部分的新鲜废物进行生物转化，将放入的废物转化成废渣。 虽然生物转化是在处置量的一部分新鲜废物上发生的，但废弃物从处置量表面以下的处置量挖掘出来。 由于挖掘发生在表面积以下，生物转化过程不受挖掘废渣的影响。 用于连续生物转化的消毒废物的装置包括具有用于容纳处置量的侧壁和底板的处置轨道。 挖掘间隙与用于从处理容积挖掘废料的侧壁中的至少一个相关联。 挖掘间隙基本上位于生活系统的下方，因此可以在生物转化的同时挖掘废物。 该装置还采用刮刀来挖掘至少一部分废渣。 刮刀具有附接到链条上用于挖掘废物并且插入在叶片之间的多个叶片是当链条绕链轮时清洁叶片的背板。 当挖掘废料时，刮板沿着处置轨道的地板移动。 地板支撑处理量和刮板，并具有过滤网，从处理量过滤水。

公开(公告)号：US06492163B1

公开(公告)日：2002-12-10

申请号：US09956172

申请日：2001-09-19

申请人： Kwang Hyun Yoo ,  Wan Kyu Choi ,  Sung Ho Shin

发明人： Kwang Hyun Yoo ,  Wan Kyu Choi ,  Sung Ho Shin

IPC分类号： C12M100

CPC分类号： C12M27/12 ,  C12M25/12 ,  Y10S435/809

摘要： A cell tube for ex vivo culturing animal cells and a multiple roller tube cell culture system are disclosed. Suitable for use in culturing cells, the tube has at its opposite end walls two openings through which culture media can come in and out. The openings are eccentrically located at corresponding positions in contact with the edge sides of the end walls. The system has a plurality of roller drums on which a multitude of cell tubes are assembled. As the roller drums are rotated, cells adhering to each tube experience a nutrient-rich state and aerobic starvation, repeatedly. In the nutrient-rich state, the cells are grown flourishingly. When subjected to starvation, cells select metabolism pathways for utilizing carbon sources effectively, produce lactate at a low rate and can maintain a constant pH, because they are in direct contact with air. The system makes cells adhere to the wall of cell tubes and provides air directly to cell surfaces. Adherent cells can be grown with normal morphology at high yield for a long time in the cell culture system. The cell culture can be easily scaled up simply by increasing the number of the roller drums. The system can exchange media and feed a gas mixture of oxygen and carbon dioxide easily, thus providing optimal environments suitable for small to large scale cell culture.

摘要翻译： 公开了用于离体培养动物细胞的细胞管和多辊管细胞培养系统。 适合用于培养细胞，该管在其相对端壁具有两个开口，培养基可通过这些开口进出。 开口偏心地位于与端壁的边缘侧接触的相应位置处。 该系统具有多个滚筒，多个细胞管组装在该滚筒上。 随着滚筒的旋转，粘附在每个管上的细胞反复出现营养丰富的状态和有氧饥饿。 在营养丰富的状态下，细胞生长旺盛。 当受到饥饿时，细胞选择有效利用碳源的代谢途径，以低的速率生产乳酸盐，并且由于它们与空气直接接触而能保持恒定的pH。 该系统使细胞粘附到细胞管壁上，并将空气直接提供给细胞表面。 粘附细胞可以在细胞培养系统中长时间以高产率以正常形态生长。 通过增加滚筒的数量，可以容易地放大细胞培养物。 该系统可以容易地交换介质并供给氧气和二氧化碳的气体混合物，从而提供适合于小型至大规模细胞培养的最佳环境。

公开(公告)号：US06451590B1

公开(公告)日：2002-09-17

申请号：US09775316

申请日：2001-02-01

申请人： Jeffrey W. Adelberg ,  Maria P. Delgado

发明人： Jeffrey W. Adelberg ,  Maria P. Delgado

IPC分类号： C12M100

CPC分类号： A01G9/026

摘要： A plant propagation apparatus is disclosed which comprises a flexible transparent enclosure. The enclosure is disposable, and could be formed of a polymer such as polyethylene. The enclosure would have at least two walls, formed of flexible material that facilitates transmission of light through the walls. Plant material is sealed within the enclosure, and nutrient solution is made available within the sealed enclosure for the plant. The enclosure is a low cost and effective method for propagating young plants and transporting them between the laboratory environment and a greenhouse or field. The enclosure may be configured to receive gas or carbon dioxide on its inner surface to a predetermined level of pressure, thereby affording the enclosure a flexible but stiff mechanical resistance that protects the young plant while it is growing.

摘要翻译： 公开了一种植物繁殖设备，其包括柔性透明外壳。 外壳是一次性的，并且可以由诸如聚乙烯的聚合物形成。 外壳将具有至少两个由柔性材料形成的壁，其有助于透过壁透过光。 植物材料密封在外壳内，营养液在植物的密封外壳内可用。 外壳是一种低成本和有效的方法，用于传播年轻植物，并在实验室环境和温室或田间之间运输。 外壳可以被配置成将气体或二氧化碳在其内表面上接收到预定的压力水平，从而为外壳提供柔性但刚性的机械阻力，以保护年轻植物在其生长时保护。

公开(公告)号：US06399367B1

公开(公告)日：2002-06-04

申请号：US09796034

申请日：2001-02-28

申请人： Robert Pollock ,  Jeffrey W. Adelberg ,  Charles Fox ,  Gianpaolo Bonaca

发明人： Robert Pollock ,  Jeffrey W. Adelberg ,  Charles Fox ,  Gianpaolo Bonaca

IPC分类号： C12M100

CPC分类号： A01G9/24 ,  Y02A40/264 ,  Y10S47/03

摘要： A system and apparatus for growing plants is disclosed. The system includes a first thermal panel with multiple flow channels, the thermal panel being located between a growing space and a light source. A cooling fluid is circulated in a countercurrent flow pattern (i.e. in at least two different directions). The channels may be connected to one or more headers, which serve as a fluid manifold to deliver fluid and collect fluid from the flow channels. Growing closely spaced plants from seed or cuttings to transplants for the field or a greenhouse is one application of this system.

摘要翻译： 公开了用于种植植物的系统和装置。 该系统包括具有多个流动通道的第一热面板，热面板位于生长空间和光源之间。 冷却流体以逆流流动模式（即在至少两个不同方向）循环。 通道可以连接到一个或多个集管，其用作流体歧管以输送流体并从流动通道收集流体。 生长紧密的植物从种子或切割到田间或温室的移植是该系统的一个应用。

公开(公告)号：US06284529B1

公开(公告)日：2001-09-04

申请号：US09284012

申请日：1999-06-08

申请人： Pietro Carrera

发明人： Pietro Carrera

IPC分类号： C12M100

CPC分类号： C05F17/027 ,  C05F17/0235 ,  Y02P20/129 ,  Y02P20/145 ,  Y02W30/43

摘要： A plant for the biological treatment of solid organic waste, where the organic mass is fed into a treatment tank with a horizontal bottom, while screw propellers, suspended from a sliding bridge parallel to tank base, operate immersed in the matter and whose axis is inclined according to a convenient angle, preferably between 5° and 50°, compared to its vertical, moving the organic matter from the bottom upwards and from the inflowing side of the treatment tank to the outflowing one, continuously immersed in a direction which is at right angles compared to the direction of movement of the organic mass and remaining continuously active, that is without any idle return of the screw propellers across the treatment tank after each process cycle.

摘要翻译： 用于生物处理固体有机废物的植物，其中将有机质量进料到具有水平底部的处理罐中，而从与罐底平行的滑动桥悬挂的螺旋桨螺旋桨操作浸没在物体中并且其轴线倾斜 根据方便的角度，优选在5°和50°之间，与其垂直方向相比，将有机物从底部向上和从处理罐的流入侧移动到流出的一侧，连续地浸入在右侧的方向上 角度与有机质量的运动方向相比，并保持连续有效，即在每个处理循环之后螺旋桨螺旋桨在整个处理槽中没有任何怠速返回。