公开(公告)号：US06638734B1

公开(公告)日：2003-10-28

申请号：US09591694

申请日：2000-06-09

申请人： John C. Reed ,  Shu-ichi Matsuzawa

发明人： John C. Reed ,  Shu-ichi Matsuzawa

IPC分类号： C12P206

CPC分类号： C07K14/00

摘要： In accordance with the present invention, there are provided novel Siah-Mediated-Degradation-Proteins (SMDPs) and/or SCF-Complex Proteins (SCPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SMDPs and/or SCPs can be employed in a variety of ways, for example, for the production of anti-SMDP and/or SCP antibodies thereto, in therapeutic compositions, and methods employing such proteins and/or antibodies for drug screening, functional genomics and other applications. Also provided are transgenic non-human mammals that express the invention protein.

摘要翻译： 根据本发明，提供了新型的Siah介导的降解蛋白（SMDP）和/或SCF-复合蛋白（SCP）。 还公开了编码这种蛋白质的核酸序列和使用其的测定法。 本发明SMDP和/或SCP可以以各种方式使用，例如用于在治疗组合物中生产抗SMDP和/或SCP抗体，以及使用这种蛋白质和/或抗体进行药物筛选的方法， 功能基因组学等应用。 还提供了表达本发明蛋白质的转基因非人哺乳动物。

公开(公告)号：US06458561B1

公开(公告)日：2002-10-01

申请号：US09523849

申请日：2000-03-13

申请人： Olga Bandman ,  Angela Molteni ,  Paola Magnaghi ,  Roberta Bosotti ,  Emanuela Scacheri ,  Antonella Isacchi ,  David M. Hodgson

发明人： Olga Bandman ,  Angela Molteni ,  Paola Magnaghi ,  Roberta Bosotti ,  Emanuela Scacheri ,  Antonella Isacchi ,  David M. Hodgson

IPC分类号： C12P206

CPC分类号： C12N9/1205

摘要： The invention provides a nucleic acid molecule which encodes the human NIM1 kinase. It also provides for the use of the nucleic acid molecule, fragments, variants and complements thereof and of the protein, portions thereof and antibodies thereto for characterization, diagnosis, evaluation, treatment, or prevention of disorders associated with expression. The invention additionally provides expression vectors and host cells for the production of the protein and a transgenic organism or model system.

摘要翻译： 本发明提供了编码人NIM1激酶的核酸分子。 它还提供使用核酸分子，其片段，变体和互补体以及蛋白质，其部分及其抗体来表征，诊断，评价，治疗或预防与表达相关的病症。 本发明另外提供用于产生蛋白质的表达载体和宿主细胞和转基因生物体或模型系统。

公开(公告)号：US06448041B1

公开(公告)日：2002-09-10

申请号：US09740235

申请日：2000-12-18

申请人： Amy K. Wolven ,  Randi E. Krasnow ,  Bridget A. Warren ,  Mariah R. Baughn

发明人： Amy K. Wolven ,  Randi E. Krasnow ,  Bridget A. Warren ,  Mariah R. Baughn

IPC分类号： C12P206

CPC分类号： C07K14/4748 ,  A01K2217/05 ,  A01K2217/075 ,  A61K38/00 ,  C12Q1/6886 ,  C12Q2600/158

摘要： The invention provides a cDNA which encodes a colon cancer marker. It also provides for the use of the cDNA, fragments, complements, and variants thereof and of the encoded protein, portions thereof and antibodies thereto for diagnosis and treatment of colon disorders, particularly colon cancer and polyps. The invention additionally provides expression vectors and host cells for the production of the protein and a transgenic model system.

摘要翻译： 本发明提供编码结肠癌标志物的cDNA。 它还提供使用cDNA，其片段，补体和变体，以及编码的蛋白质，其部分及其抗体用于诊断和治疗结肠疾病，特别是结肠癌和息肉。 本发明另外提供用于产生蛋白质的表达载体和宿主细胞和转基因模型系统。

公开(公告)号：US06358709B1

公开(公告)日：2002-03-19

申请号：US09522289

申请日：2000-03-09

申请人： Jay M. Short ,  Gerhard Johann Frey

发明人： Jay M. Short ,  Gerhard Johann Frey

IPC分类号： C12P206

CPC分类号： C07K14/445 ,  A61K39/00 ,  A61K2039/53 ,  C12N9/00 ,  C12N9/14 ,  C12N9/16 ,  C12N9/88 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/1034 ,  C12Q1/6811 ,  C12Y301/11002

摘要： This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of end-selection-based methods is the ability to recover full-length polynucleotides from a library of progeny molecules generated by mutagenesis methods. These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis™) and non-stochastic polynucleotide reassembly (GeneReassembly™). This invention provides methods of obtaining novel enzymes that have optimized physical &/or biological properties. Through use of the claimed methods, genetic vaccines, enzymes, small molecules, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression, increased uptake into a cell, increased stability in a cell, ability to tailor an immune response, and the like. Furthermore, this invention provides methods of obtaining a variety of novel biologically active molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.

摘要翻译： 本发明提供了通过使用定向进化（DirectEvolution TM）的非随机方法获得新的多核苷酸和编码的多肽的方法。 基于终端选择的方法的特别优点是从通过诱变方法产生的后代分子的文库中恢复全长多核苷酸的能力。 这些方法包括非随机多核苷酸位点饱和诱变（Gene Site Saturation Mutagenesis TM）和非随机多核苷酸重组（GeneReassembly TM）。 本发明提供获得具有优化的物理和/或生物学特性的新型酶的方法。 通过使用所要求保护的方法，可以将遗传疫苗，酶，小分子和其它所需分子演变成期望的性质。 例如，可以获得表现出增加用作基因疫苗的功效的疫苗载体。 通过使用该方法获得的载体可具有例如增强的抗原表达，增加的细胞摄取，增加细胞的稳定性，定制免疫应答的能力等。 此外，本发明提供了在抗生素，药物治疗剂和转基因性状领域中获得各种新型生物活性分子的方法。