公开(公告)号：US07135298B2

公开(公告)日：2006-11-14

申请号：US10401058

申请日：2003-03-26

申请人： Robert T. Abraham

发明人： Robert T. Abraham

IPC分类号： G01N33/53 ,  G01N33/567 ,  C12Q1/33 ,  C12P1/00 ,  C12N9/00

CPC分类号： G01N33/573 ,  G01N2500/00 ,  Y10T436/25

摘要： The present invention provides an assay for the identification of agents which can modulate TOR-mediated phosphorylation of substrate proteins. The assays of the invention utilize substrate proteins whose amino acid sequence contains the Ser/Thr motif recognized by TOR. Naturally occurring TOR which may be used in the methods of the invention include TOR isolated from a variety of species, particularly mammalian tissues.

摘要翻译： 本发明提供了用于鉴定可调节底物蛋白质的TOR介导的磷酸化的试剂的测定法。 本发明的测定法利用其氨基酸序列含有被TOR识别的Ser / Thr基序的底物蛋白质。 可用于本发明方法的天然存在的TOR包括从各种物种特别是哺乳动物组织分离的TOR。