公开(公告)号：US06465778B1

公开(公告)日：2002-10-15

申请号：US09364244

申请日：1999-07-29

申请人： Claus Koster ,  Jochen Franzen

发明人： Claus Koster ,  Jochen Franzen

IPC分类号： H01J4404

CPC分类号： H01J49/164 ,  G01N1/22

摘要： The invention relates to ionization with low fragmentation and adduct formation, of high-molecular analyte molecules, particularly of large biopolymers, by matrix-assisted laser desorption (MALDI) from liquid matrices for mass-spectrometric analysis of the resulting ions, particularly for determination of their molecular weight. The invention uses liquids as matrix substances with very low vapor pressures from the group of minimally trihydroxylic alcohols with at least one ether bond (ether polyols or polyether polyols). These can be used advantageously for energy absorption and ionization by infrared lasers (IR-MALDI), however other lasers, such as conventional. UV lasers, can be used for other wavebands by the addition of light-absorbing compounds.

摘要翻译： 本发明涉及通过基质辅助激光解吸（MALDI）从液体基质中获得高分子分析物分子，特别是大生物聚合物的低分裂和加合物形成的离子，用于所得离子的质谱分析，特别是用于确定 它们的分子量。 本发明使用液体作为具有非常低蒸气压的基质物质，其具有至少一个醚键的最小三羟基醇（醚多元醇或聚醚多元醇）。 这些可以有利地用于通过红外激光器（IR-MALDI）的能量吸收和电离，然而其他激光器，例如常规的。 UV激光器可以通过添加光吸收化合物用于其他波段。

公开(公告)号：US06265716B1

公开(公告)日：2001-07-24

申请号：US09515357

申请日：2000-02-29

申请人： Joanna M. Hunter ,  Hua Lin ,  Christopher H. Becker

发明人： Joanna M. Hunter ,  Hua Lin ,  Christopher H. Becker

IPC分类号： H01J4404

CPC分类号： H01J49/164 ,  G01N1/22 ,  H01J49/0468

摘要： A sample preparation method is disclosed for volatilization and mass spectrometric analysis of nonvolatile high molecular weight molecules. Photoabsorbing molecules having significant sublimation rates at room temperature under vacuum, and preferably containing hydroxy functionalities, are disclosed for use as matrices in matrix-assisted laser desorption/ionization mass spectrometry. The samples are typically cooled in the mass spectrometer to temperatures significantly below room temperature.

摘要翻译： 公开了用于非挥发性高分子量分子的挥发和质谱分析的样品制备方法。 公开了在真空下，优选含有羟基官能团的具有显着升华速率的光吸收分子，用作基质辅助激光解吸/电离质谱法中的基质。 样品通常在质谱仪中冷却至明显低于室温的温度。