公开(公告)号：WO2015014858A1

公开(公告)日：2015-02-05

申请号：PCT/EP2014/066312

申请日：2014-07-29

Applicant: OVIZIO IMAGING SYSTEMS NV/SA

Inventor： JOORIS, Serge ,  MATHUIS, Philip

IPC: C12M1/00 ,  C12M1/34 ,  G01N15/14

CPC classification number: B01L3/50273 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50825 ,  B01L2200/0647 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12M23/38 ,  C12M23/50 ,  C12M41/36 ,  G01N21/84

Abstract: The current invention concerns a vessel for storing and monitoring a suspension of microscopic objects, comprising a cap suitable for closing off the vessel, whereby the cap comprises a fluidics system comprising an inlet and an outlet, said inlet and said outlet arranged to be in an interior volume of said vessel when the cap is closing off said vessel, whereby said cap comprises a measurement region for optical microscopy which comprises a sample region suitable for said suspension, the sample region in fluid connection with said fluidics system, whereby said cap comprises pumping means suitable for inducing a flow in said fluidics system. The invention also concerns a monitoring system and setup, in particular for monitoring cells in a cell culture liquid.

Abstract translation: 本发明涉及一种用于存储和监测微观物体的悬浮液的容器，其包括适于封闭容器的盖，由此所述盖包括流体系统，所述流体系统包括入口和出口，所述入口和所述出口布置成 当帽盖关闭所述容器时，所述容器的内部容积，由此所述盖包括用于光学显微镜的测量区域，其包括适于所述悬浮液的样品区域，所述样品区域与所述流体系统流体连接，由此所述盖子包括泵送 适于诱导所述流体系统中的流动的装置。 本发明还涉及一种监测系统和装置，特别是用于监测细胞培养液中的细胞。

公开(公告)号：WO2013120886A1

公开(公告)日：2013-08-22

申请号：PCT/EP2013/052852

申请日：2013-02-13

Applicant: OVIZIO IMAGING SYSTEMS NV/SA

Inventor： JOORIS, Serge ,  MATHUIS, Philip

IPC: G01N15/14

CPC classification number: G01N15/1434 ,  G01N15/1404 ,  G01N15/1459 ,  G01N21/6486 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1445 ,  G03H1/0005 ,  G03H1/0443 ,  G03H2001/0033 ,  G03H2001/005

Abstract: The current invention concerns a flow cytometric system and method for observing, analyzing and/or separating objects in a liquid sample, comprising a digital holographic microscope (DHM) and at least one fluidic system, whereby the DHM comprises illumination means, an interferometric system and digital recording means, whereby the fluidic system is capable of guiding said objects through an illumination beam of the illumination means of said DHM, whereby the fluidic system comprises a mechanism for inducing a liquid sample stream through the fluidic system, whereby preferably the fluidic system comprises a stream size controlling device for controlling the transverse dimensions of a liquid sample stream inside said fluidic system, preferably said stream size controlling device is capable of lining up the objects one-by-one or multiple objects at a time in said liquid sample stream.

Abstract translation: 本发明涉及用于观察，分析和/或分离液体样品中的物体的流式细胞术系统和方法，其包括数字全息显微镜（DHM）和至少一个流体系统，由此所述DHM包括照明装置，干涉系统和 数字记录装置，由此流体系统能够通过所述DHM的照明装置的照明光束引导所述物体，由此流体系统包括用于将液体样品流引导通过流体系统的机构，由此优选地，流体系统包括 用于控制所述流体系统内的液体样品流的横向尺寸的料流尺寸控制装置，优选地，所述料流尺寸控制装置能够在所述液体样品流中一次一个或多个物体排列物体。

公开(公告)号：WO2013011000A1

公开(公告)日：2013-01-24

申请号：PCT/EP2012/063935

申请日：2012-07-16

Applicant: Ovizio Imaging Systems NV/SA ,  JOORIS, Serge ,  MATHUIS, Philip

Inventor： JOORIS, Serge ,  MATHUIS, Philip

IPC: G01N21/45 ,  G01B9/021 ,  G03H1/00

CPC classification number: G01N21/453 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/005 ,  G03H2001/0445 ,  G03H2001/0447 ,  G06F17/30525 ,  G06K9/00134

Abstract: The present invention discloses a method for analyzing a liquid cell sample, comprising the steps of: a) providing at least one liquid cell sample in a sample vial; b) obtaining data linked to the cells in the sample by performing differential digital holographic microscopy on said liquid cell sample in said sample vial. In a second aspect, the invention also provides for a system for analyzing a liquid cell sample, comprising (i) a differential digital holographic microscope comprising illumination means, a differential interferometer and a digital recording device connected to a processing device such as a computer; (ii) at least one exchangeable sample vial comprising a liquid cell (iii) a movable sample vial holder; characterized in that (iv) said sample vial holder is adapted to receive said sample vial; (v) said sample vial holder is adapted to position said sample vial such that the focal plane of the objective lens of said differential digital holographic microscope lies in the vial.

Abstract translation: 本发明公开了一种用于分析液体细胞样品的方法，包括以下步骤：a）在样品瓶中提供至少一个液体细胞样品; b）通过在所述样品瓶中的所述液体细胞样品上进行差分数字全息显微镜来获得与样品中细胞相连的数据。 在第二方面，本发明还提供了一种用于分析液体细胞样品的系统，包括（i）差分数字全息显微镜，包括照明装置，差分干涉仪和连接到诸如计算机的处理装置的数字记录装置; （ii）至少一个可替换的样品瓶，其包含液体池（iii）可移动的样品瓶保持器; 其特征在于（iv）所述样品瓶保持器适于接收所述样品瓶; （v）所述样品瓶保持器适于定位所述样品瓶，使得所述差分数字全息显微镜的物镜的焦平面位于小瓶中。

公开(公告)号：WO2023062193A1

公开(公告)日：2023-04-20

申请号：PCT/EP2022/078658

申请日：2022-10-14

Applicant: OVIZIO IMAGING SYSTEMS NV/SA

Inventor： LEBACQ, André

IPC: G05D11/00 ,  B01F23/40

Abstract: Method for monitoring a liquid culture, wherein the monitoring comprises inline dilution of a flow of the liquid culture, comprising: providing a device (100) for inline mixing of two or more liquid flows, wherein each liquid flow is induced by a separate pump (202, 204), the device (100) comprising a liquid culture inlet flow channel (112) for conducting the liquid culture flow from a separate pump (202); and a diluent inlet flow channel (114) for conducting a diluent flow from a separate pump (204); a mixer (120) into which liquid from the inlet flow channels (112, 114) enters and is mixed, wherein passage of liquid flow through each inlet flow channel (112, 114) effects a reduction in periodic variability in liquid flow rate, diluting the liquid culture flow with the diluent flow using the device (100), thereby obtaining an inline diluted liquid culture, monitoring, from the diluted liquid culture, the liquid culture.

公开(公告)号：WO2014044823A1

公开(公告)日：2014-03-27

申请号：PCT/EP2013/069625

申请日：2013-09-20

Applicant: OVIZIO IMAGING SYSTEMS NV/SA

Inventor： MATHUIS, Philip ,  JOORIS, Serge

IPC: G01N21/05 ,  G03H1/00 ,  G01N15/14

CPC classification number: G03H1/0005 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/025 ,  B01L2200/143 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0877 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2300/168 ,  B01L2400/0481 ,  C12M41/36 ,  G02B21/0016 ,  G02B21/26 ,  G03H1/0443 ,  G03H2001/005 ,  G03H2001/0204

Abstract: The current invention concerns a fluid microscope system for analyzing and/or monitoring the contents of one or more fluid-based reactors or canalizations such as bio-reactors, micro-reactors, brewing reactors, water supply systems or sewer systems, comprising a digital holographic microscope (DHM) capable of obtaining phase information of a fluid sample and comprising illumination means and one or more fluidic systems connected to said reactors and to said DHM, capable of guiding fluid from said reactors to said DHM,whereby at least one fluidic system comprises one or more tubes which may come in direct contact with fluid from said reactor, characterized in that at least one tube comprises a part which is at least partially transparent for the illumination means of said DHM for obtaining holographic information of said fluid sample. The current invention also concerns a tube and a fluidic system for such a fluid microscope.

Abstract translation: 本发明涉及用于分析和/或监测一个或多个基于流体的反应器或管道的内容物的流体显微镜系统，例如生物反应器，微反应器，冲泡反应器，供水系统或下水道系统，包括数字全息 显微镜（DHM），其能够获得流体样品的相位信息，并且包括照明装置和连接到所述反应器和所述DHM的一个或多个流体系统，其能够将流体从所述反应器引导到所述DHM，由此至少一个流体系统包括 一个或多个管可以与来自所述反应器的流体直接接触，其特征在于，至少一个管包括对于所述DHM的照明装置至少部分透明的部分，用于获得所述流体样品的全息信息。 本发明还涉及用于这种流体显微镜的管和流体系统。