METHODS AND DEVICES FOR IMAGING OBJECTS ON A MICROFLUIDIC CHIP
    8.
    发明申请
    METHODS AND DEVICES FOR IMAGING OBJECTS ON A MICROFLUIDIC CHIP 审中-公开
    用于在微流控芯片上成像对象的方法和装置

    公开(公告)号:WO2018052730A1

    公开(公告)日:2018-03-22

    申请号:PCT/US2017/049731

    申请日:2017-08-31

    IPC分类号: B01L3/00 G01N33/569 C12M1/12

    摘要: Methods and systems for controlling a microscope platform for determining cells of interest in a sample containing a plurality of cells, each cell being disposed in a through hole on one of a plurality of filters on a microfluidic chip, each of the plurality of filters having an active area including a plurality of through holes arranged in a pattern. One method includes generating a first image of a microfluidic chip filter, determining location information for candidate cells of interest that are disposed in filter through holes, aligning the microscope platform to capture an image of each candidate cell of interest using the stored location information to align the microscope platform to capture an image at the location of the through hole where the candidate cell of interest is disposed, and generating a second image, higher than the resolution of the first image, depicting each candidate cell of interest.

    摘要翻译: 用于控制显微镜平台的方法和系统,所述显微镜平台用于确定含有多个细胞的样本中的感兴趣细胞,每个细胞设置在微流体芯片上的多个过滤器中的一个过滤器上的通孔中, 多个滤光器中的每一个具有有源区域,有源区域包括以图案排列的多个通孔。 一种方法包括生成微流控芯片过滤器的第一图像,确定布置在过滤器通孔中的感兴趣候选细胞的位置信息,对齐显微镜平台以使用所存储的位置信息来对准每个感兴趣候选细胞的图像以对准 所述显微镜平台在所述感兴趣的候选细胞所处的通孔位置处捕获图像,并生成高于所述第一图像的分辨率的第二图像,描绘每个感兴趣的候选细胞。

    A COMMON-PATH INTERFEROMETRIC SCATTERING IMAGING SYSTEM AND A METHOD OF USING COMMON-PATH INTERFEROMETRIC SCATTERING IMAGING TO DETECT AN OBJECT
    9.
    发明申请
    A COMMON-PATH INTERFEROMETRIC SCATTERING IMAGING SYSTEM AND A METHOD OF USING COMMON-PATH INTERFEROMETRIC SCATTERING IMAGING TO DETECT AN OBJECT 审中-公开
    一种公用路径干涉散射成像系统及一种利用公共路径干涉测量成像检测物体的方法

    公开(公告)号:WO2018019934A1

    公开(公告)日:2018-02-01

    申请号:PCT/EP2017/068997

    申请日:2017-07-27

    摘要: The present invention relates to a common-path interferometric scattering imaging system and a method of using such a system, where the system comprises: - illuminating means for emitting an illumination beam (L 0 ); - light collecting means for collecting through a common collection optical path: - a scattered beam (L s ) provided by the light scattering on an object (T) of the illumination beam (L 0 ); and - a reference beam (L r ) provided by the reflection on or transmission through an interface (I) of the illumination beam (L 0 ); - image sensing means (D) for receiving and sensing the collected scattered (L s ) and reference (L r ) beams interfering thereon as an interferometric light signal; - attenuation means arranged in the common collection optical path for attenuating the reference beam (L r ) before it arrives at the image sensing means (D); and - processing means (P) to process data corresponding to the interferometric light signal.

    摘要翻译: 本发明涉及共路干涉式散射成像系统和使用这种系统的方法,其中该系统包括: - 照明装置,用于发射照明光束(L0 <0) /子>); - 光收集装置,用于通过公共收集光路收集: - 由散射在照明光束(L 0)上的物体(T)上的光提供的散射光束(L s) /子>); 和 - 通过在照明光束(L0)的界面(I)上的反射或透射而提供的参考光束(Lr)。 - 图像感测装置(D),用于接收和感测在其上干涉的收集的散射(L s)和参考(L r)光束作为干涉光信号; - 设置在公共收集光路中的衰减装置,用于在参考光束(Lr)到达图像传感装置(D)之前衰减参考光束(Lr)。 和 - 处理装置(P),用于处理对应于干涉光信号的数据。

    METHOD FOR ANALYZING A CELL PELLET
    10.
    发明申请
    METHOD FOR ANALYZING A CELL PELLET 审中-公开
    分析细胞球的方法

    公开(公告)号:WO2018019383A1

    公开(公告)日:2018-02-01

    申请号:PCT/EP2016/068115

    申请日:2016-07-28

    申请人: CUREVAC AG

    摘要: The present invention relates to method for analyzing a cell pellet, a device for analyzing a cell pellet and a system for analyzing a cell pellet. Further, several uses as described herein are part of the present invention. The method for analyzing a cell pellet comprises the following steps: a) providing an image comprising the cell pellet, b) determining a radial dimension of the cell pellet based on the image, and c) calculating an amount of cells in the cell pellet based on the determined radial dimension of the cell pellet and a predefined cell type constant.

    摘要翻译: 本发明涉及分析细胞团的方法,分析细胞团的装置和分析细胞团的系统。 此外,如本文所述的几种用途是本发明的一部分。 用于分析细胞沉淀物的方法包括以下步骤:a)提供包含细胞沉淀物的图像,b)基于图像确定细胞沉淀物的径向尺寸,以及c)基于细胞沉淀物计算基于细胞沉淀物的细胞量 在确定的细胞沉淀的径向尺寸和预定的细胞类型常数上。