摘要:
The present disclosure relates to methods, systems and computer program products for detecting biological cells and/or tracing a cell lineage forest of biological cells. The tracing or detecting comprises obtaining multiple microscopy images, in particular lens-free microscopy images, depicting the biological cells. It further comprises generating a probability map for one of the microscopy images, the probability map indicating probabilities for the presence of the biological cells at multiple regions, in particular pixels, of the one microscopy image, and/or determining, based on multiple generated probability maps, one for each of the multiple microscopy images, a cell lineage forest indicating the descent and/or the localisation of at least some of the biological cells over the sequence of the multiple microscopy images.
摘要:
Die Erfindung betrifft ein Verfahren zum Ausgeben einer Flüssigkeit. Das Verfahren ist dadurch gekennzeichnet, dass die Flüssigkeit aus einer Ausgabevorrichtung ausgegeben und eine Istposition der Flüssigkeit nach Ausgabe der Flüssigkeit aus der Ausgabevorrichtung ermittelt wird.
摘要:
A method of preparing a histopathology sample using a container with an internal volume. A divider is placed in the container so as to divide the internal volume into separate compartments. A tissue specimen is placed in at least one of the compartments. Paraffin or another embedding medium in a liquid state is added to the internal volume of the container such that the divider material and the specimen are at least partially immersed in the medium. The medium is then allowed to solidify, to create a unit. The unit can then be sliced along a plane that intersects the specimen, the divider material, and the medium to create a thin section of the unit that comprises the specimen, the divider material, and the medium, and that is adapted to be observed using a microscope or other observation equipment.
摘要:
Disclosed herein is a housing for a filter array, comprising: a first (e.g., upper) susface, a second (e.g., lower) surface, and a periphery, which are configured to house (e.g., enclose) a filter array comprising a plurality of filters; and for each filter of the array, at least two pre-filtration ports on the first surface which are connected io a pre-filtration chamber of the filter, and at least two posi-filtraiion ports on the second surface which are connected to a post-filtration chamber of the filter. A seating for the housing, the housing/seating assembly, a base plate comprising the assembly, a device comprising the base plate, and a method of using the device for filtering a sample are also disclosed.
摘要:
Provided herein are devices including tunable nanoscale confinement in combination with electrophoresis, and methods and for analysis of biological analytes using such devices.
摘要:
Methods and devices for automated gating of flow cytometry data, including: sorting the flow cytometry data according to one or more parameters measured by a flow cytometer; determining a density model of the sorted flow cytometry data; calculating a derivative value at each of a plurality of points on the density model; determining, using the calculated derivative values: one or more peaks in the density model, and one or more points of inflection in the density model; and identifying one or more gating points based on the one or more peaks or the one or more points of inflection.
摘要:
L'invention est un procédé de détermination d'une quantité de leucocytes dans un échantillon. Le procédé est basé sur une image, ou hologramme, de l'échantillon obtenue par un capteur d'image lorsque l'échantillon est illuminé par une source de lumière. L'hologramme fait l'objet d'une reconstruction holographique, de façon à obtenir une image complexe, dite image complexe de référence, représentant une onde lumineuse transmise par l'échantillon, dans un plan de reconstruction. A cette image complexe de référence est appliqué un opérateur de propagation holographique, de façon à obtenir une pluralité d'images complexes dites secondaires, formant une pile d'images, à partir desquelles on détermine un profil décrivant l'évolution d'une caractéristique optique de l'onde lumineuse transmise par l'échantillon selon l'axe de propagation cette onde lumineuse. La présence de leucocytes et leur quantité sont déterminées en fonction de ces profils.
摘要:
Methods and systems for controlling a microscope platform for determining cells of interest in a sample containing a plurality of cells, each cell being disposed in a through hole on one of a plurality of filters on a microfluidic chip, each of the plurality of filters having an active area including a plurality of through holes arranged in a pattern. One method includes generating a first image of a microfluidic chip filter, determining location information for candidate cells of interest that are disposed in filter through holes, aligning the microscope platform to capture an image of each candidate cell of interest using the stored location information to align the microscope platform to capture an image at the location of the through hole where the candidate cell of interest is disposed, and generating a second image, higher than the resolution of the first image, depicting each candidate cell of interest.
摘要:
The present invention relates to a common-path interferometric scattering imaging system and a method of using such a system, where the system comprises: - illuminating means for emitting an illumination beam (L 0 ); - light collecting means for collecting through a common collection optical path: - a scattered beam (L s ) provided by the light scattering on an object (T) of the illumination beam (L 0 ); and - a reference beam (L r ) provided by the reflection on or transmission through an interface (I) of the illumination beam (L 0 ); - image sensing means (D) for receiving and sensing the collected scattered (L s ) and reference (L r ) beams interfering thereon as an interferometric light signal; - attenuation means arranged in the common collection optical path for attenuating the reference beam (L r ) before it arrives at the image sensing means (D); and - processing means (P) to process data corresponding to the interferometric light signal.
摘要:
The present invention relates to method for analyzing a cell pellet, a device for analyzing a cell pellet and a system for analyzing a cell pellet. Further, several uses as described herein are part of the present invention. The method for analyzing a cell pellet comprises the following steps: a) providing an image comprising the cell pellet, b) determining a radial dimension of the cell pellet based on the image, and c) calculating an amount of cells in the cell pellet based on the determined radial dimension of the cell pellet and a predefined cell type constant.