摘要:
Die Erfindung betrifft einen Produktionsstamm zur Herstellung von 2,3-Butandiol. Der Produktionsstamm weist eine 2 bis 68 fach über dem Ausgangsstamm liegende Acetolactatsynthase Aktivität auf. Die Erfindung betrifft ferner ein Verfahren zur fermentativen Herstellung von 2,3-Butandiol mittels dieses Produktionsstammes.
摘要:
Crabtree positive yeast cells that have endogenous expressed pyruvate decarboxylase genes inactivated and an engineered biosynthetic pathway utilizing pyruvate were found to have improved growth and product yield when glucose repression was reduced. These cells were able to grow in media containing a high glucose concentration.
摘要:
The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.
摘要:
The present invention relates to a method of producing methionine in Coryneform bacteria in which enzymes of the pentose phosphate pathway are over - expressed. The present invention also relates to Coryneform bacteria for producing methionine in which at least two enzymes of the pentose phosphate pathway are over - expressed.
摘要:
The present invention relates to microorganisms genetically engineered to reduce carbon source diversion throughout the bioconversion of a carbon source, such as e.g. sorbitol, into biomass. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. sorbitol, into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. sorbitol.
摘要:
The present invention provides genetically manipulated cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD, hnr or yifP gene, or further disrupted for a gdhA, gpmB aceE,
摘要:
The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B 2 ), FAD, FMN, pyridoxal phosphate (vitamin B 6 ), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.
摘要翻译:本发明涉及一种生物技术生产化合物的改进方法,其中核糖-5-磷酸,核酮糖-5-磷酸或木酮糖-5-磷酸是生物合成前体,如核黄素(维生素B 2 N 2) ,FAD,FMN,磷酸吡哆醛(维生素B 6 S),鸟苷,GMP,腺苷,AMP。 本发明还涉及产生这些化合物的生物体的产生。 此外,还涉及产生突变的转酮酶,其允许在葡萄糖上正常生长,但当被引入生产菌株中时葡萄糖酸盐的生长减少,以及编码它们的多核苷酸。
摘要:
Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
摘要:
The present invention provides genetically manipulated cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD , hnr or yifP gene, or further disrupted for a gdhA , gpmB aceE , ppc , talB or fdhF gene, or any combination thereof, or cells inhibited for their expression, activity or function are disclosed. Methods of enhanced carotenoid synthesis utilizing cells genetically engineered to overexpress dxs , idi , yjiD , rpoS , torC , appY , ydgK , yeiA , yedR , tort , arcB , yggT , purDH , yfjN or a combination thereof, or further disrupted for the above-referenced genes are disclosed. Methods for identifying genes involved in optimized production of a carotenoid, and cells disrupted for, or, inhibited for the expression, activity or function of genes thus identified are described.
摘要:
Die Erfindung betrifft transgene Expressionskonstrukte und Verfahren zum Erhöhen des Vitamin E-Gehaltes in pflanzlichen Organismen, bevorzugt in Algen, durch transgene Expression von ORF sll0529 aus Synechocystis sp. PCC6803. Die Erfindung betrifft ferner transgene Expressionskonstrukte zur Expression von ORF sll0529 in pflanzlichen Organismen, bevorzugt in Algen, transgene pflanzlichen Organismen exprimierend sll0529, sowie die Verwendung von besagten transgenen pflanzlichen Organismen zur Herstellung von Nahrungs-, Futtermitteln, Saatgut, Pharmazeutika oder Feinchemikalien, insbesondere zur Herstellung von Vitamin E.