VERFAHREN ZUR FERMENTATIVEN HERSTELLUNG VON 2,3-BUTANDIOL
    91.
    发明申请
    VERFAHREN ZUR FERMENTATIVEN HERSTELLUNG VON 2,3-BUTANDIOL 审中-公开
    PROCEDURE发酵生产2,3-丁二醇

    公开(公告)号:WO2012104243A1

    公开(公告)日:2012-08-09

    申请号:PCT/EP2012/051426

    申请日:2012-01-30

    发明人: PFALLER, Rupert

    IPC分类号: C12N1/21 C12N9/10 C12P7/18

    摘要: Die Erfindung betrifft einen Produktionsstamm zur Herstellung von 2,3-Butandiol. Der Produktionsstamm weist eine 2 bis 68 fach über dem Ausgangsstamm liegende Acetolactatsynthase Aktivität auf. Die Erfindung betrifft ferner ein Verfahren zur fermentativen Herstellung von 2,3-Butandiol mittels dieses Produktionsstammes.

    摘要翻译: 本发明涉及一种生产菌株用于生产2,3-丁二醇。 生产菌株具有2-68倍的亲本菌株的乙酰乳酸合酶活性更高。 本发明还涉及通过这种生产菌株的手段发酵生产2,3-丁二醇的方法。

    ALTERNATIVE BUTANOL PRODUCTION PROCESS IN A MICROBIAL CELL
    93.
    发明申请
    ALTERNATIVE BUTANOL PRODUCTION PROCESS IN A MICROBIAL CELL 审中-公开
    一种微生物细胞中的替代丁醇生产工艺

    公开(公告)号:WO2010031772A2

    公开(公告)日:2010-03-25

    申请号:PCT/EP2009/061961

    申请日:2009-09-15

    摘要: The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.

    摘要翻译: 本发明涉及包含编码酶的基因的微生物细胞,所述酶催化下列反应a)丙酮酸转化为乙酰乳酸,b)乙酰乳酸转化为2,3-二羟基异戊酸,c)2,3-二羟基异戊酸转化成2 d)2-酮异戊酸酯与异丁酰-CoA,e)异丁酰-CoA与丁酰-CoA,f)丁酰-CoA与丁醛,和g)丁醛与丁醇,其中所述细胞产生丁醇。 本发明还涉及生产丁醇的方法,其中使用了根据本发明的微生物细胞。

    BIOLOGICAL PROCESS USING A TRANSALDOLASE
    95.
    发明申请
    BIOLOGICAL PROCESS USING A TRANSALDOLASE 审中-公开
    使用转移酶的生物过程

    公开(公告)号:WO2008000415A1

    公开(公告)日:2008-01-03

    申请号:PCT/EP2007/005591

    申请日:2007-06-25

    摘要: The present invention relates to microorganisms genetically engineered to reduce carbon source diversion throughout the bioconversion of a carbon source, such as e.g. sorbitol, into biomass. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. sorbitol, into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. sorbitol.

    摘要翻译: 本发明涉及基因工程改造以减少在碳源的整个生物转化过程中碳源转移的微生物,例如, 山梨醇,成为生物质。 产生这种微生物的方法也由本发明提供。 本发明还涉及包含编码参与碳源的生物转化的蛋白质的基因的多核苷酸序列,例如碳源。 山梨醇,成为生物质。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸,由多核苷酸及其片段编码的新多肽及其功能等同物。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法,所述宿主微生物导致具有降低的碳源转移的微生物,即来自碳源的生物质产量的较高产量和/或效率,例如。 山梨糖醇。

    MODIFIED TRANSKETOLASE AND USE THEREOF
    97.
    发明申请
    MODIFIED TRANSKETOLASE AND USE THEREOF 审中-公开
    改性的转铁蛋白酶及其用途

    公开(公告)号:WO2007051552A1

    公开(公告)日:2007-05-10

    申请号:PCT/EP2006/010270

    申请日:2006-10-25

    发明人: LEHMANN, Martin

    IPC分类号: C12N9/10 C12P25/00

    摘要: The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B 2 ), FAD, FMN, pyridoxal phosphate (vitamin B 6 ), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.

    摘要翻译: 本发明涉及一种生物技术生产化合物的改进方法,其中核糖-5-磷酸,核酮糖-5-磷酸或木酮糖-5-磷酸是生物合成前体,如核黄素(维生素B 2 N 2) ,FAD,FMN,磷酸吡哆醛(维生素B 6 S),鸟苷,GMP,腺苷,AMP。 本发明还涉及产生这些化合物的生物体的产生。 此外,还涉及产生突变的转酮酶,其允许在葡萄糖上正常生长,但当被引入生产菌株中时葡萄糖酸盐的生长减少,以及编码它们的多核苷酸。

    GENE TARGETS FOR ENHANCED CAROTENOID PRODUCTION
    99.
    发明申请
    GENE TARGETS FOR ENHANCED CAROTENOID PRODUCTION 审中-公开
    用于增强毛细血管生成的基因目标

    公开(公告)号:WO2005062923A2

    公开(公告)日:2005-07-14

    申请号:PCT/US2004/043295

    申请日:2004-12-23

    摘要: The present invention provides genetically manipulated cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD , hnr or yifP gene, or further disrupted for a gdhA , gpmB aceE , ppc , talB or fdhF gene, or any combination thereof, or cells inhibited for their expression, activity or function are disclosed. Methods of enhanced carotenoid synthesis utilizing cells genetically engineered to overexpress dxs , idi , yjiD , rpoS , torC , appY , ydgK , yeiA , yedR , tort , arcB , yggT , purDH , yfjN or a combination thereof, or further disrupted for the above-referenced genes are disclosed. Methods for identifying genes involved in optimized production of a carotenoid, and cells disrupted for, or, inhibited for the expression, activity or function of genes thus identified are described.

    摘要翻译: 本发明提供了遗传操作的细胞及其利用方法。 使用对yjiD,hnr或yifP基因遗传破坏的细胞的增强的类胡萝卜素合成方法,或进一步破坏gdhA,gpmB aceE,ppc,talB或fdhF基因或其任何组合,或抑制其表达,活性或功能的细胞 被披露。 使用经过遗传工程改造以过表达dxs,idi,yjiD,rpoS,torC,appY,ydgK,yeiA,yedR,tort,arcB,yggT,purDH,yfjN或其组合的细胞的类胡萝卜素合成增强方法, 参考的基因被公开。 描述了鉴定参与优化的类胡萝卜素生产的基因的方法,以及由此鉴定的基因破坏或抑制表达的活性或功能的细胞的方法。