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    胚様体形成用容器及び胚様体の形成方法
    45.
    发明申请
    胚様体形成用容器及び胚様体の形成方法 审中-公开
    用于形成层的容器和形成层的方法

    公开(公告)号：WO2005001019A1

    公开(公告)日：2005-01-06

    申请号：PCT/JP2004/008970

    申请日：2004-06-25

    Applicant: 日本油脂株式会社 ,  黒澤 尋 ,  榊 秀次郎

    Inventor： 黒澤 尋 ,  榊 秀次郎

    IPC: C12M3/00

    CPC classification number: C12N5/0603 ,  C12M21/06 ,  C12M23/20 ,  C12N2533/20 ,  C12N2533/30

    Abstract: 　煩雑な手法を用いることなく、容易にES細胞より胚様体を形成するために使用する胚様体形成用容器及び該容器を用いた、容易で効率良い胚様体の形成方法であって、該形成方法は、ES細胞を浮遊培養するための領域を形成するための容器表面に、特定のPC類似基を有する化合物を用いて形成した被覆層を備える胚様体形成用容器を準備する工程(A)と、該容器内において、胚様体を形成するためにES細胞を浮遊培養する工程(B)とを含む。

    Abstract translation: 用于胚层形成的容器，其用于从ES细胞容易地形成胚层，而不用纠缠程序; 以及容易且有效地形成使用容器的胚层的方法。 具体而言，形成方法包括步骤（A），其提供用于生成胚层的容器，该容器具有由容器表面上具有化合物的特定PC-类似基团形成的涂层，用于形成ES细胞悬浮培养区域 以及进行ES细胞的悬浮培养以在容器内形成胚层的步骤（B）。

    METHOD AND APPARATUS FOR MULTI-LAYER GROWTH OF ANCHORAGE-DEPENDENT CELLS
    46.
    发明申请
    METHOD AND APPARATUS FOR MULTI-LAYER GROWTH OF ANCHORAGE-DEPENDENT CELLS 审中-公开
    用于依赖于锚定的细胞的多层生长的方法和装置

    公开(公告)号：WO02044341A2

    公开(公告)日：2002-06-06

    申请号：PCT/US2001/047061

    申请日：2001-11-13

    IPC: C12N5/00 ,  C12N

    CPC classification number: C12M23/24 ,  C12M23/06 ,  C12M23/20 ,  C12M23/48 ,  C12M35/08 ,  C12N5/0068 ,  C12N2533/20 ,  C12N2533/54

    Abstract: Anchorage-dependent cells are grown in a novel cell culture plate and on a novel substratum which increase the oxygenation of the cells. The cell culture plate is made by enclosing a growth chamber within a shell made of a solid sterilizable. One or more culture wells are positioned within the chamber. An inlet port and outlet port are fashioned within the shell for gas exchange. The wells have a well wall which allows for the diffusion of oxygen from the chamber into the well. A perfluorocarbon is placed within the well. A perfluoro-aldehyde is mixed with the perfluorocarbon, and the perfluoro-aldehyde re-orients so that the aldehyde head groups are at the interface. An attachment factor is bound to the perfluoro-aldehyde, which is sunk into the PFC substratum. Aqueous growth media is then added to the well, and anchorage-dependent cells added and allowed to grow.

    Abstract translation: 根据本发明，锚定依赖性细胞在新的细胞培养板中和新的底物上培养以增加细胞的氧合作用。 细胞培养板通过将生长室放置在由固体可消毒材料制成的壳中来制备。 该室内有一个或多个培养孔。 在该壳体上提供进气口和出气口以用于气体交换。 井包括一个将氧气从室内扩散到井中的井。 全氟化碳放置在井内。 全氟醛与该全氟化碳混合，全氟醛被重新定向，使得醛头基团位于界面处。 附着因子与浸入PFC衬底的全氟醛有关。 然后将水性生长培养基加入到孔中，并且锚定依赖性细胞可以自然发育。

    NOVEL SYSTEM FOR MULTILAYER GROWTH OF ANCHORAGE-DEPENDENT CELLS
    47.
    发明申请
    NOVEL SYSTEM FOR MULTILAYER GROWTH OF ANCHORAGE-DEPENDENT CELLS 审中-公开
    ANGORAGE-DEPENDENT细胞多层生长的新系统

    公开(公告)号：WO9700941A2

    公开(公告)日：1997-01-09

    申请号：PCT/US9611149

    申请日：1996-06-24

    Applicant: UNIV UTAH RES FOUND

    Inventor： TRUJILLO EDWARD M ,  RAPPAPORT CATHERINE

    IPC: C12M3/00 ,  C12N5/00 ,  C12M

    CPC classification number: C12M23/20 ,  C12M25/06 ,  C12N5/0068 ,  C12N2533/20 ,  C12N2533/54

    Abstract: Anchorage-dependent cells are grown on a novel substratum which is belived to increase the oxygenation of the cells and reduce the formation of free-radicals. The substratum consists of a perfluorocarbon reservoir which is coated with a layer of protein (e.g., collagen or gelatin) which has been perfluoroalkylated. The perfluoroalkylated protein forms a very stable surface to which anchorage-dependent cells can attach and grow. Using this system, mammalian cell cultures have been grown to densities exceeding 10 cells/cm . The increased density is attributed to the formation of multiple cell layers which resemble tissue-like structures.

    Abstract translation: 锚定依赖的细胞生长在一个新的基质上，这种细胞被认为是增加细胞的氧合作用并减少自由基的形成。 该基质由全氟化碳储存器组成，其全层烷基化被涂覆有一层蛋白质（例如胶原或明胶）。 全氟烷基化蛋白质形成非常稳定的表面，锚定依赖性细胞可附着和生长。 使用该系统，哺乳动物细胞培养物已经生长至超过10 7个细胞/ cm 2的密度。 增加的密度归因于形成类似组织样结构的多个细胞层。

    FULLERENE COATED SURFACES AND USES THEREOF
    48.
    发明申请
    FULLERENE COATED SURFACES AND USES THEREOF 审中-公开
    FULLERENE涂层表面及其用途

    公开(公告)号：WO1994000552A1

    公开(公告)日：1994-01-06

    申请号：PCT/US1993005680

    申请日：1993-06-14

    Applicant: THE TRUSTEES OF DARTMOUTH COLLEGE

    Inventor： THE TRUSTEES OF DARTMOUTH COLLEGE ,  RICHMOND, Robert, C. ,  GIBSON, Ursula, J.

    IPC: C12M03/04

    CPC classification number: C12N5/0068 ,  C12M23/20 ,  C12N15/87 ,  C12N2533/20 ,  Y10S977/738 ,  Y10S977/847 ,  Y10S977/905 ,  Y10S977/923

    Abstract: Substrates having a surface coated with fullerene and a substance attached thereto are disclosed. Cell culture substrates having a fullerene-coated surface are useful in methods of growing cells on the fullerene-coated surface. Methods of preparing cell culture substrates for cell attachment and growth by coating a surface with fullerene are provided. Cells can be grown on a fullerene-coated surface in the presence of a substance such as a cytokine, growth hormone or a drug, to evaluate the interaction between the substance and the cells. Methods for increasing cell membrane permeability and for introducing a substance, such as a DNA or RNA vector, into a cell are also provided.

    Abstract translation: 公开了具有涂覆有富勒烯的表面和附着于其上的物质的底物。 具有富勒烯涂覆表面的细胞培养基底可用于在富勒烯涂覆的表面上生长细胞的方法。 提供了通过用富勒烯涂覆表面来制备用于细胞附着和生长的细胞培养基质的方法。 细胞可以在诸如细胞因子，生长激素或药物的物质存在下在富勒烯涂覆的表面上生长，以评估物质和细胞之间的相互作用。 还提供了增加细胞膜通透性并将物质如DNA或RNA载体导入细胞的方法。

    METHOD FOR CELL CULTURE
    49.
    发明申请
    METHOD FOR CELL CULTURE 审中-公开
    细胞培养方法

    公开(公告)号：WO1985003520A1

    公开(公告)日：1985-08-15

    申请号：PCT/AU1985000018

    申请日：1985-02-06

    Applicant: SURFACE CONCEPTS PTY. LTD. ,  KJELLEBERG, Staffan ,  KEFFORD, Bruce

    Inventor： SURFACE CONCEPTS PTY. LTD.

    IPC: C12N11/08

    CPC classification number: C12N5/0068 ,  C12N1/00 ,  C12N2533/20

    Abstract: Processes for the surface culture of eukaryotic and procaryotic cells on solid carriers. The growth cells are cultured on or in the presence of a solid carrier coated with surface active molecules which act as a major carbon- and energy source. The surface active molecules which have very low solubility in an aqueous solution, are hold in position at the solid surface by hydrophobic forces. The process can be considered as an immobilization of the surface active molecules. The surface active molecules include phospholipids, triglycerides, fatty acids, proteins, synthetic peptides and sterol. The aqueous medium can be any convenient medium without foetal calf serum or albumin-compounds or with reduced amounts of these. The invention also includes products for healing skin leasions.

    Abstract translation: 固体载体上真核细胞和原核细胞表面培养的方法。 生长细胞在涂覆有作为主要碳源和能量源的表面活性分子的固体载体或存在下培养。 在水溶液中具有非常低的溶解度的表面活性分子通过疏水力保持在固体表面的位置。 该方法可以被认为是表面活性分子的固定化。 表面活性分子包括磷脂，甘油三酯，脂肪酸，蛋白质，合成肽和甾醇。 水性培养基可以是任何方便的培养基，不含胎牛血清或白蛋白化合物或其量减少。 本发明还包括用于治疗皮肤感染的产品。

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