公开(公告)号：WO2020069129A9

公开(公告)日：2020-04-02

申请号：PCT/US2019/053154

申请日：2019-09-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： DOUDNA, Jennifer, A. ,  CAO, Yuhong ,  MA, Enbo ,  YANG, Peidong

IPC: A61N1/30 ,  A61N1/32 ,  C12N5/04 ,  C12N5/07 ,  C12N5/071 ,  C12N5/10 ,  C12N5/14 ,  C12N5/16 ,  C12N15/06 ,  C12N15/07 ,  C12N15/87

Abstract: The present disclosure provides devices and methods for delivering a biomolecule into a cell. A delivery device of the present disclosure includes a first reservoir, a second reservoir, a porous membrane comprising a nanopore, and two or more electrodes configured to generate an electric field across the porous membrane for delivery of a biomolecule present in the second reservoir through the nanopore of the porous membrane and into a cell present in the first reservoir.

公开(公告)号：WO2019233462A1

公开(公告)日：2019-12-12

申请号：PCT/CN2019/090228

申请日：2019-06-06

Applicant: 浙江海正博锐生物制药有限公司

Inventor： 王海彬 ,  胡锋 ,  张轩 ,  焦静雨 ,  吴振华 ,  高栋

IPC: C07K16/28 ,  A61K39/00 ,  C12N5/10 ,  C12N15/06 ,  A61P35/00

Abstract: 本发明公开了针对程序性死亡配体（PD-L1）的抗体及其应用，特别是在治疗和/或预防PD-L1相关疾病中的应用。

公开(公告)号：WO2019104311A1

公开(公告)日：2019-05-31

申请号：PCT/US2018/062580

申请日：2018-11-27

Applicant: BROWN UNIVERSITY ,  REENAN, Robert ,  SAHIN, Asli ,  SAVVA, Yiannis

Inventor： REENAN, Robert ,  SAHIN, Asli ,  SAVVA, Yiannis

IPC: A01K67/027 ,  C12N9/02 ,  C12N15/06 ,  C12N15/53 ,  C12Q1/26 ,  C12Q1/68

Abstract: The present invention features isolated Cyp4gl polypeptides comprising mutations at positions corresponding to amino acid positions 92, 112, 143, and 333 in a Drosophila melanogaster Cyp4gl polypeptide. In some embodiments, the mutations are G92E, D112N, S143N, and M333I. The mutations suppress a lethal effect of a SODl G85R allele. The present invention further features polynucleotides encoding these polypeptides, as well as methods of suppressing or treating a neurological disease such as amyotrophic lateral sclerosis (ALS) using compositions comprising polypeptides and/or polynucleotides of the invention.

公开(公告)号：WO2018226642A1

公开(公告)日：2018-12-13

申请号：PCT/US2018/035984

申请日：2018-06-05

Applicant: ORGANOFAB TECHNOLOGIES, INC.

Inventor： FLITSCH, Robert ,  FLITSCH, Frederick ,  CHANIN, Brent ,  HILKEN, Rudiger

IPC: B29C64/171 ,  B29C64/10 ,  C12N5/071 ,  C12N15/06 ,  G05B19/418 ,  G05B19/4099 ,  G05B19/4097 ,  B33Y50/02

Abstract: The present invention provides apparatus and methods for production of tissue structures and organs. In some examples, a clean space facility may be equipped with modelling hardware and software, nanotechnology and microelectronic apparatus, and additive manufacturing equipment to print cells and support matrix to allow cells to grow into tissue structures and organs. Various methods relating to using and producing the tissue engineering system are discussed.

公开(公告)号：WO2017197425A1

公开(公告)日：2017-11-23

申请号：PCT/AU2016/050372

申请日：2016-05-18

Applicant: HUDSON INSTITUTE OF MEDICAL RESEARCH

Inventor： ST JOHN, Justin Charles

IPC: C12N5/075 ,  C12N15/06 ,  C12N15/01

Abstract: The present disclosure relates to a method for facilitating embryogenesis potential via manipulation of a female mammalian germ cell, in particular an oocyte. Taught herein is a method for enhancing the potential of an oocyte fertilized in vitro to develop to term following implantation.

Abstract translation: 本公开涉及通过操纵雌性哺乳动物生殖细胞，特别是卵母细胞促进胚胎发生潜能的方法。 在此教导的是一种用于增强体外受精的卵母细胞在植入后发育至期的潜力的方法。

公开(公告)号：WO2017123830A1

公开(公告)日：2017-07-20

申请号：PCT/US2017/013285

申请日：2017-01-13

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： PERRINO, Fred W.

IPC: A61K39/00 ,  A61P37/02 ,  A61P37/06 ,  C12N15/06 ,  C12N15/09

CPC classification number: A01K67/0275 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/0325

Abstract: Provided herein is a recombinant or transgenic non-human mammal comprising a nucleic acid encoding a mutant three-prime exonuclease 1 (three prime repair exonuclease 1; TREX1), and in particular aspects the mammal expresses the mutant Trex1 protein. The non-human mammal is useful for identifying candidate compounds for the treatment of autoimmune disease (in human or animal, typically mammalian) subjects. Another aspect of the invention is, accordingly, a method for indentifying candidate compounds for the treatment of autoimmune disease or disorder comprising: providing the recombinant non-human mammal; administering a test substance to the recombinant non-human mammal; and determining whether said test substance reduces at least one indicia of autoimmune disease in said mammal, wherein a reduction in said at least one indicia indicates said test substance is a candidate compound for the treatment of autoimmune disease.

Abstract translation: 本文提供了包含编码突变三基因外切核酸酶1（三种主要修复外切核酸酶1; TREX1）的核酸的重组或转基因非人哺乳动物，并且在特定方面，所述哺乳动物表达突变体 Trex1蛋白。 非人哺乳动物可用于鉴定用于治疗自身免疫性疾病（在人或动物，典型地哺乳动物）受试者中的候选化合物。 因此，本发明的另一个方面是用于鉴定用于治疗自身免疫性疾病或病症的候选化合物的方法，其包括：提供重组非人类哺乳动物; 将测试物质施用于重组非人类哺乳动物; 并确定所述测试物质是否降低所述哺乳动物中至少一种自身免疫性疾病的标记，其中所述至少一种标记的减少表明所述测试物质是用于治疗自身免疫疾病的候选化合物。

公开(公告)号：WO2017088018A1

公开(公告)日：2017-06-01

申请号：PCT/AU2016/051147

申请日：2016-11-23

Applicant: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION ,  UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.

Inventor： BEAN, Andrew ,  LOWENTHAL, John William ,  MALAVER-ORTEGA, Luis Fernando ,  TRIPP, Ralph A

IPC: C12N15/00 ,  C12N7/00 ,  A61K39/00 ,  C12N15/06 ,  A61K39/155 ,  A61K39/145 ,  A61K39/17

CPC classification number: C12N7/00 ,  C12N2760/16051 ,  C12N2760/16151 ,  C12N2760/18251

Abstract: The present invention relates to methods of replicating viruses in vitro . In particular, the invention relates to a genetically modified population of cells, and/or a population of cells treated with an exogenous compound, wherein the cells are capable of producing more virus than cells lacking the genetic modification and/or lacking treatment with the exogenous compound. The invention also relates to methods of producing populations of such cells, as well as the use of the viruses obtained to prepare vaccine compositions.

Abstract translation: 本发明涉及在体外复制病毒的方法。 具体而言，本发明涉及经遗传修饰的细胞群和/或用外源性化合物处理的细胞群，其中所述细胞比缺乏所述遗传修饰和/或缺乏外源处理的细胞能够产生更多的病毒 复合。 本发明还涉及生产这些细胞群体的方法，以及使用获得的病毒制备疫苗组合物。

公开(公告)号：WO2014040405A1

公开(公告)日：2014-03-20

申请号：PCT/CN2013/072295

申请日：2013-03-07

Applicant: 赵永祥

Inventor： 卢小玲 ,  彭宜

IPC: C12N15/06

CPC classification number: C12N5/16 ,  A61K39/0011 ,  A61K2039/5152 ,  A61K2039/5154 ,  C12N2500/50 ,  C12N2502/1121 ,  C12N2502/30

Abstract: 本发明公开了一种诱导树突状细胞与肿瘤细胞融合的方法，其在融合过程中依次添加一定量的聚乙二醇和胶原蛋白来高效诱导细胞融合，其中聚乙二醇能使两细胞接触点处质膜的脂类分子发生疏散和重组，由于两细胞接口处双分子层质膜的相互亲和以及彼此的表面张力作用，从而使细胞发生融合。胶原蛋白可以使融合后细胞膜紧缩，修复细胞膜，从而形成稳定、高效的融合细胞，适用于生产肿瘤疫苗、单克隆抗体等特定的生物制品。

公开(公告)号：WO2013177255A3

公开(公告)日：2014-03-13

申请号：PCT/US2013042178

申请日：2013-05-22

Applicant: CONNECTICUT COLLEGE

Inventor： BRANCHINI BRUCE

IPC: C12N9/02 ,  C12N15/06

CPC classification number: C12N9/0069 ,  A61K49/0045 ,  A61K49/0097 ,  C07K2319/00 ,  C07K2319/61 ,  C12Q1/66 ,  C12Y113/12007

Abstract: Described herein are novel chimeric luciferase molecules with enhanced properties, and methods of using these chimeric luciferase molecules.

Abstract translation: 本文描述了具有增强性质的新型嵌合荧光素酶分子以及使用这些嵌合荧光素酶分子的方法。

公开(公告)号：WO2013134931A1

公开(公告)日：2013-09-19

申请号：PCT/CN2012/072284

申请日：2012-03-13

Applicant: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES ,  SU, Guannan ,  ZHAO, Yannan ,  WEI, Jianshu ,  CHEN, Bing ,  XIAO, Zhifeng ,  DAI, Jianwu

Inventor： SU, Guannan ,  ZHAO, Yannan ,  WEI, Jianshu ,  CHEN, Bing ,  XIAO, Zhifeng ,  DAI, Jianwu

IPC: C12N5/02 ,  C12N5/07 ,  C12N5/071 ,  C12N5/073 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/09 ,  C12N5/10 ,  C12N15/06 ,  C12N15/07

CPC classification number: C12N5/0062 ,  C12N5/0619 ,  C12N2500/30 ,  C12N2506/00 ,  C12N2533/76

Abstract: Methods for reprogramming cells by culturing the cells under a condition that allows formation of a three-dimensional cell aggregate are provided. The cells and cell aggregates obtained using the methods are also provided.

Abstract translation: 提供了在允许形成三维细胞聚集体的条件下通过培养细胞来重编程细胞的方法。 还提供了使用这些方法获得的细胞和细胞聚集体。