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    MICROORGANISMS AND PROCESSES FOR ENHANCED PRODUCTION OF PANTOTHENATE
    41.
    发明申请
    MICROORGANISMS AND PROCESSES FOR ENHANCED PRODUCTION OF PANTOTHENATE 审中-公开
    增强生物活性的微生物和方法

    公开(公告)号：WO2004005525A3

    公开(公告)日：2006-02-02

    申请号：PCT/US0321305

    申请日：2003-07-03

    申请人： BASF AG ,  YOCUM R ROGERS ,  PATTERSON THOMAS A ,  PERO JANICE G ,  HERMANN THERON

    发明人： YOCUM R ROGERS ,  PATTERSON THOMAS A ,  PERO JANICE G ,  HERMANN THERON

    IPC分类号： C12N1/21 ,  C12P13/02 ,  C12P1/00 ,  C07H21/04 ,  C12N9/00 ,  C12N9/10 ,  C12P19/02

    CPC分类号： C12N15/52 ,  C12P13/02

    摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

    摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增加所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    NITRILE HYDRATASE AND A METHOD FOR PRODUCING AMIDES
    45.
    发明申请
    NITRILE HYDRATASE AND A METHOD FOR PRODUCING AMIDES 审中-公开
    NITRILE HYDRATASE和一种生产AMIDES的方法

    公开(公告)号：WO02070717A3

    公开(公告)日：2003-03-27

    申请号：PCT/JP0201912

    申请日：2002-03-01

    申请人： DAICEL CHEM ,  NAGASAWA TOHRU ,  MATSUYAMA AKINOBU

    发明人： NAGASAWA TOHRU ,  MATSUYAMA AKINOBU

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/88 ,  C12N15/60 ,  C12P13/02 ,  C12P17/00 ,  C12R1/01

    CPC分类号： C12P13/02 ,  C12N9/88

    摘要： An objective of the present invention is to provide a nitrile hydratase capable of producing 2-hydroxy-4-methylthiobutyroamide. The present invention provides a novel nitrile hydratase producing alpha -hydroxyamide using alpha -hydroxynitrile as the substrate, and the encoding DNA thereof. The enzyme can be obtained from Rhodococcus sp. Further, the enzymatic activity of the enzyme can be maintained stably during the reaction. The present invention provides a method for producing amide compounds,the method comprising the step of reacting this enzyme to nitrile compounds. According to the present invention, from hydroxy nitrile compounds, corresponding amide compounds can be produced biochemically without reducing the enzyme activity of nitrile hydratase.

    摘要翻译： 本发明的目的是提供能够生产2-羟基-4-甲硫基丁酰胺的腈水合酶。 本发明提供使用α-羟基腈作为底物产生α-羟基酰胺的新型腈水合酶及其编码DNA。 该酶可以从红球菌（Rhodococcus sp。）获得。 此外，酶的酶活性可以在反应期间保持稳定。 本发明提供了一种制备酰胺化合物的方法，所述方法包括使该酶与腈化合物反应的步骤。 根据本发明，由羟基腈化合物可以生物化学地制备相应的酰胺化合物，而不会降低腈水合酶的酶活性。

    PROCESS FOR THE FERMENTATIVE PREPARATION OF D-PANTOTHENIC ACID AND/OR SALTS THEREOF
    46.
    发明申请
    PROCESS FOR THE FERMENTATIVE PREPARATION OF D-PANTOTHENIC ACID AND/OR SALTS THEREOF 审中-公开
    D-泛血酸和/或其盐的发酵制备方法

    公开(公告)号：WO2003004673A1

    公开(公告)日：2003-01-16

    申请号：PCT/EP2002/005961

    申请日：2002-05-31

    申请人： DEGUSSA AG ,  KRUSE, Daniela ,  THIERBACH, Georg

    发明人： KRUSE, Daniela ,  THIERBACH, Georg

    IPC分类号： C12P13/02

    CPC分类号： C12P13/02

    摘要： The invention provides a process for the fermentative preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of microorganisms of the Bacillus group, in particular those which already produce D-pantothenic acid, which comprises attenuating, eliminating or expressing at a low level in the microorganisms one or more of the nucleotide sequence(s) which code(s) for the gene or ORF azlC, azlD, ydaP and pckA or of the proteins coded by these.

    摘要翻译： 本发明提供了通过发酵芽孢杆菌属组的微生物，特别是已经产生D-泛酸的那些，包括减毒，消除或减少D-泛酸和/或其盐的发酵制剂或包含它们的饲料添加剂的方法， 在微生物中表达一个或多个编码基因或ORF azlC，azlD，ydaP和pckA的核苷酸序列或由这些编码的蛋白质的低水平。

    PROCESS FOR PRODUCING SUBSTANCE BY USING MICROBIAL CATALYST
    48.
    发明申请
    PROCESS FOR PRODUCING SUBSTANCE BY USING MICROBIAL CATALYST 审中-公开
    使用微生物催化剂生产物质的方法

    公开(公告)号：WO02052027A1

    公开(公告)日：2002-07-04

    申请号：PCT/JP2000/009138

    申请日：2000-12-22

    IPC分类号： C12M1/38 ,  C12N1/21 ,  C12P1/00 ,  C12P13/02 ,  C12P7/42 ,  C12P11/00

    CPC分类号： C12P13/02 ,  C12P1/00

    摘要： An industrially advantageous process for producing a substance wherein the production speed of a reaction product in a reaction with the use of a microbial catalyst can be maintained at a constant level over a long period of time. In a reaction with the use of a microbial catalyst (for example, resting cells of an arthrobacter capable of converting a-hydroxynitriles into the corresponding alpha -hydroxy acid amides or alpha -hydroxy acid ammonium salts), the substance is produced at a constant speed by adding the substrate at a constant speed, regulating the concentration of the microbial catalyst in the liquid reaction mixture at a constant level and controlling the temperature so as to rise the reaction temperature at the point that the enzymatic activity of the microbial catalyst is lowered in the course of the reaction so as to maintain the reaction speed at a constant level.

    摘要翻译： 一种工业上有利的生产物质的方法，其中使用微生物催化剂的反应中的反应产物的生产速度可以长时间保持在恒定水平。 在使用微生物催化剂（例如能够将α-羟基腈转化为相应的α-羟基酸酰胺或α-羟基酸铵盐的节肢动物的静止细胞）的反应中，物质以恒定速度 通过以恒定的速度添加基材，以一定水平调节液体反应混合物中的微生物催化剂的浓度，并控制温度，以使微生物催化剂的酶活性降低的点升高反应温度 反应过程以使反应速度保持在恒定的水平。

    PROCESS FOR PRODUCING AMIDE COMPOUND BY USING MICROBIAL CATALYST
    49.
    发明申请
    PROCESS FOR PRODUCING AMIDE COMPOUND BY USING MICROBIAL CATALYST 审中-公开
    使用微生物催化剂生产酰胺化合物的方法

    公开(公告)号：WO02050297A1

    公开(公告)日：2002-06-27

    申请号：PCT/JP2001/011149

    申请日：2001-12-19

    IPC分类号： C12P13/02 ,  C12P17/12 ,  C12R1/01

    CPC分类号： C12P17/12 ,  C12P13/02

    摘要： A process for producing an amide compound from a nitrile compound by using a microbial catalyst characterized in that microbial cells having a nitrile hydratase activity of at least 50 U/mg dry cells at a reaction temperature of 10 DEG C, which are not in comprehensively immobilized, are brought into contact with a nitrile compound in an aqueous medium. According to this process in which microbial cells overexpressing the nitrile hydratase activity are used without comprehensive immobilization, the amide compound can be efficiently produced from the nitrile compound without causing the problem of lowering in the reaction speed or a decrease in the productivity per unit amount of the microbial cells due to the comprehensive immobilization. Accordingly, the amide compound can be produced within an extremely short period of time in case of a batch reaction, or by using equipment on a very small scale in case of a continuous reaction.

    摘要翻译： 一种通过使用微生物催化剂从腈化合物制备酰胺化合物的方法，其特征在于在10℃的反应温度下具有至少50U / mg干细胞的腈水合酶活性的微生物细胞，其未全面固定 在含水介质中与腈化合物接触。 根据不进行全面固定化而使用过表达腈水合酶活性的微生物细胞的方法，可以从腈化合物有效地制造酰胺化合物，而不会引起反应速度降低或单位量生产率下降的问题 微生物细胞由于全面固定。 因此，在间歇反应的情况下，酰胺化合物可以在非常短的时间内生产，或者在连续反应的情况下通过使用非常小规模的设备来制备。

    PROCESS FOR THE FERMENTATIVE PREPARATION OF D-PANTOTHENIC ACID USING CORYNEFORM BACTERIA
    50.
    发明申请
    PROCESS FOR THE FERMENTATIVE PREPARATION OF D-PANTOTHENIC ACID USING CORYNEFORM BACTERIA 审中-公开
    使用CORYNEFORM BACTERIA的D-泛酸的发酵制备方法

    公开(公告)号：WO02029020A1

    公开(公告)日：2002-04-11

    申请号：PCT/EP2001/010212

    申请日：2001-09-05

    IPC分类号： C12N9/02 ,  C12P13/02 ,  C12N1/21

    CPC分类号： C12N9/0008 ,  C12P13/02

    摘要： The invention relates to a process for the preparation of D-pantothenic acid by fermentation of coryneform bacteria, in which bacteria in which the nucleotide sequence which codes for pyruvate oxidase (EC 1.2.2.2) (poxB gene) is attenuated are employed, the following steps being carried out: a) fermentation of D-pantothenic acid-producing bacteria in which at least the gene which codes for pyruvate oxidase is attenuated. b) concentration of the D-pantothenic acid in the medium or in the cells of the bacteria; and c) isolation of the D-pantothenic acid produced.

    摘要翻译： 本发明涉及通过棒状杆菌细菌发酵制备D-泛酸的方法，其中使用编码丙酮酸氧化酶（EC 1.2.2.2）（poxB基因）的核苷酸序列被减毒的细菌，以下 正在进行的步骤：a）D-泛酸产生细菌的发酵，其中至少编码丙酮酸氧化酶的基因被减弱。 b）D-泛酸在培养基或细菌细胞中的浓度; 和c）分离产生的D-泛酸。