公开(公告)号：WO9725621A2

公开(公告)日：1997-07-17

申请号：PCT/DE9700072

申请日：1997-01-09

Applicant: FORSCHUNGSZENTRUM JUELICH GMBH ,  BERLIN PETER ,  TILLER JOERG ,  KLEMM DIETER

Inventor： BERLIN PETER ,  TILLER JOERG ,  KLEMM DIETER

IPC: G01N33/543 ,  G01N33/547 ,  G01N33/548

CPC classification number: G01N33/54353

Abstract: The invention concerns (bio)chemical reagent solid phases consisting of a polymer or macromolecular carrier compound containing NH2 to which coupling structures are covalently bound via NH2 groups, which in turn are connected to receptor and/or indicator compounds and which thereby couple the receptor and/or indicator coumpounds to the carriers. The coupling structures consist of ascorbic acid or dehydroascorbic acid or of a compound structurally similar to these substances.

Abstract translation: 本发明涉及（生物）化学试剂的固体组成的聚合物或大分子的相位，NH含有载体化合物2共价结合的耦合结构上的NH 2个基团，其与受体和/或指示器的化合物和由此受体进一步连接 - 和/或指示剂化合物，其中的抗坏血酸或脱氢抗坏血酸，或这些物质的耦合结构存在结构上相似的化合物的载体偶联。

公开(公告)号：WO2023019233A1

公开(公告)日：2023-02-16

申请号：PCT/US2022/074889

申请日：2022-08-12

Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

Inventor： KAFRI, Tal

IPC: G01N33/548 ,  G01N33/68 ,  A61K38/00 ,  A01K67/027 ,  C07K14/745

Abstract: This invention relates to recombinant aptamer 9.3t-resistant Factor IX proteins, as well as methods of making the same and methods of using the same in methods comprising non-hemophilic subjects, including methods of modeling Factor IX replacement therapy in a non-hemophilic subject and methods of identifying genetic background effects of a non-hemophilic subject on an exogenous Factor IX replacement protein. The invention also pertains to methods of modeling gene and/or protein replacement therapy in a protein non-deficient subject and methods of identifying genetic background effects of a protein non-deficient subject on an exogenous replacement protein, as well as methods of selecting aptamer-resistant recombinant proteins and selecting aptamer-sensitive protein non-deficient subjects for use in such methods as disclosed herein.

公开(公告)号：WO2022260463A1

公开(公告)日：2022-12-15

申请号：PCT/KR2022/008177

申请日：2022-06-10

Applicant: 주식회사 제우스

Inventor： 임종훈 ,  정흥수 ,  이지영

IPC: G01N33/548 ,  G01N33/552 ,  G01N33/543

Abstract: 본 발명은 표적물질 및 상기 표적물질과 특이적 결합을 하는 1차 압타머가 결합하여 형성된 1차 압타머-표적물질 복합체와 특이적 결합을 하는 2차 압타머에 관한 것이다. 또한, 본 발명은 상기 2차 압타머를 이용한 표적물질 검출방법 및 상기 2차 압타머를 포함하는 진단키트에 관한 것이다.

公开(公告)号：WO2022114767A1

公开(公告)日：2022-06-02

申请号：PCT/KR2021/017375

申请日：2021-11-24

Applicant: 주식회사 에이치피바이오

Inventor： 이승재 ,  안지영 ,  이상희 ,  박가영 ,  조희영

IPC: C12N15/115 ,  G01N33/548 ,  G01N33/68 ,  G01N33/569

Abstract: 본 발명은 코로나바이러스의 RNA 의존성 RNA 중합효소에 특이적으로 결합하는 RNA 앱타머 및 이의 용도에 관한 것이다. 구체적으로, 본 발명에 따른 RNA 앱타머는 코로나바이러스, 특히 신종 코로나바이러스 감염증(COVID-19)을 유발하는 신종 코로나바이러스의 RNA 의존성 RNA 중합효소에 특이적으로 결합함으로써, 코로나바이러스의 검출 및 진단뿐만 아니라, 이에 의해 발병하는 질병의 치료에도 유용하게 사용될 수 있다.

公开(公告)号：WO2021226036A1

公开(公告)日：2021-11-11

申请号：PCT/US2021/030590

申请日：2021-05-04

Applicant: SLINGSHOT BIOSCIENCES, INC.

Inventor： KIM, Jeffrey ,  NGUYEN, Anh Tuan ,  MILLER, Brandon

IPC: G01N33/543 ,  G01N33/544 ,  G01N33/545 ,  G01N33/548

Abstract: Compositions comprising multiple hydrogel particles having substantially the same diameter, but with each subgrouping of particles from the multiple hydrogel particles having different associated values for one or more passive optical properties that can be deconvoluted using cytometric instrumentation. Each hydrogel particle from the multiple hydrogel particles can be functionalized with a different biochemical or chemical target from a set of targets. A method of preparing hydrogel particles includes forming droplets and polymerizing the droplets, with optional functionalization.

公开(公告)号：WO2014077762A1

公开(公告)日：2014-05-22

申请号：PCT/SE2013/051325

申请日：2013-11-11

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： MALOISEL, Jean-Luc ,  RODRIGO, Gustav ,  NORÉN, Bjorn ,  KUMBHAR, Virendra

IPC: B01J20/26 ,  B01D15/08 ,  B01J20/285 ,  B01J20/289 ,  G01N30/02 ,  G01N33/544 ,  G01N33/548

CPC classification number: C07K1/165 ,  B01D15/3847 ,  B01D15/3885 ,  B01J20/265 ,  B01J20/28016 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3255 ,  B01J20/3285 ,  B01J41/20 ,  B01J2220/80 ,  C07K1/36 ,  G01N33/544 ,  B01D15/363 ,  B01D15/327

Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R 1 - L 1 – N (R 3 ) – L 2 –R, immobilized on a support, wherein R 1 is a five-or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L 1 is either a methylene group or a covalent bond; R 2 is a five-or six-membered, substituted or non-substituted ring structure; L 2 is either a methylene group or a covalent bond;R 3 is a methyl group; and wherein if R 1 is a hydroxyethyl group and L 1 is a covalent bond, R 2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

Abstract translation: 本发明公开了一种分离基质，其包含固定在载体上的式R1-L1-N（R3）-L2-R定义的多个分离配体，其中R1是五或六元的取代或非取代的 取代的环结构或羟乙基或羟丙基; L1是亚甲基或共价键; R2是五或六元，取代或未取代的环结构; L2是亚甲基或共价键; R3是甲基; 并且其中如果R 1是羟乙基并且L 1是共价键，则R 2是取代的芳环结构或取代或未取代的脂族环结构。

公开(公告)号：WO2012139089A3

公开(公告)日：2012-11-29

申请号：PCT/US2012032695

申请日：2012-04-09

Applicant: GEN HOSPITAL CORP ,  VYAS JATIN M ,  TAM JENNY M ,  MANSOUR MICHAEL K

Inventor： VYAS JATIN M ,  TAM JENNY M ,  MANSOUR MICHAEL K

IPC: G01N33/548 ,  A61K31/716 ,  A61K36/06 ,  A61P37/02 ,  C12Q1/04 ,  G01N33/554

CPC classification number: G01N33/5091 ,  A61K39/0002 ,  A61K47/6931 ,  G01N33/56961

Abstract: The present invention provides for compositions and methods for a purified fungal carbohydrate-linked polymer bead platform, which can serve as an artificial fungus-like particle to measure specific patient immune responses to fungal carbohydrate antigens. A specific embodiment comprises a purified ß-1,3-glucan chemically conjugated to a polymer bead.

Abstract translation: 本发明提供了用于纯化的真菌碳水化合物连接的聚合物珠平台的组合物和方法，其可以用作人造真菌样颗粒以测量对真菌碳水化合物抗原的特定患者免疫应答。 一个具体的实施方案包含化学缀合至聚合物珠的纯化的β-1,3-葡聚糖。

公开(公告)号：WO2010139863A1

公开(公告)日：2010-12-09

申请号：PCT/FI2010/050463

申请日：2010-06-04

Applicant: VALTION TEKNILLINEN TUTKIMUSKESKUS ,  TEERINEN, Tuija ,  LAINE, Janne ,  ORELMA, Hannes ,  KURKINEN, Marika

Inventor： TEERINEN, Tuija ,  LAINE, Janne ,  ORELMA, Hannes ,  KURKINEN, Marika

IPC: G01N33/548

CPC classification number: G01N33/54353 ,  G01N33/548

Abstract: The present invention concerns a new method for antibody immobilization, which is based on the conjugation of antibodies to a molecule, such as carboxymethylcellulose (CMC), and depositing the conjugate or the still unconjugated molecule on the cellulose surface. The carboxy groups of, e.g., the CMC serve as conjugation sites for the antibodies. The invention also concerns a cellulose based biointerface, which may be manufactured using said method, containing proteins deposited on a cellulose surface, as well as the use of this biointerface for detecting a desired target from a sample.

Abstract translation: 本发明涉及用于抗体固定的新方法，其基于抗体与分子例如羧甲基纤维素（CMC）的缀合，并将缀合物或仍未缀合的分子沉积在纤维素表面上。 例如CMC的羧基用作抗体的缀合位点。 本发明还涉及基于纤维素的生物界面，其可以使用所述方法制造，其含有沉积在纤维素表面上的蛋白质，以及使用该生物界面从样品中检测所需目标物。

公开(公告)号：WO2010001162A1

公开(公告)日：2010-01-07

申请号：PCT/GB2009/050765

申请日：2009-07-01

Applicant: ENIGMA DIAGNOSTICS LTD ,  FRANKS, Felix ,  SPIERS, Samantha ,  LEE, Martin ,  SUTTON, Diane

Inventor： FRANKS, Felix ,  SPIERS, Samantha ,  LEE, Martin ,  SUTTON, Diane

IPC: C12Q1/68 ,  G01N33/548

CPC classification number: C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: The use of raffinose as a glass-forming agent for freeze-dried compositions intended for use in conducting chemical or biochemical reactions such as the PCR. Thus, for instance, there is provided a composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried formand comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessaryfor conducting said chemical or biochemical reaction, and (ii) raffinose. Kits comprising these compositions and methods of using them form a further aspect of the invention.

Abstract translation: 使用棉子糖作为用于进行化学或生物化学反应如PCR的冷冻干燥组合物的玻璃形成剂。 因此，例如，提供了用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包含（i）一组试剂，其包含用于进行所述化学品所必需的至少一些化学或生化试剂 或生化反应，和（ii）棉子糖。 包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。

公开(公告)号：WO2009084481A1

公开(公告)日：2009-07-09

申请号：PCT/JP2008/073200

申请日：2008-12-19

Applicant: 株式会社ビーエル ,  難波 靖治

Inventor： 難波 靖治

IPC: G01N33/569 ,  C07K16/12 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/553 ,  C12P21/08

CPC classification number: C07K16/1289 ,  G01N33/5695

Abstract: 【課題】生体試料中の結核菌群特異的分泌タンパク質MPT64抗原を特異的に検出することにより、結核菌による感染の診断を従来よりも高い精度で迅速かつ安全に行う。 【解決手段】配列番号２乃至４の何れか１つのアミノ酸配列に位置するMPB64のエピトープを認識する抗体、特に、モノクローナル抗体を得た。かくして、当該抗体を用いる免疫測定法、特にMPB64に対する第一の抗体と第二の抗体とを用いたサンドイッチ式免疫測定法、特に、イムノクロマトグラフィー測定法、イムノクロマト法テストストリップが提供される。生体試料を、培養すること無く、または、該試料中の結核菌群菌体が実質的に増殖するに至らない時間だけ培養した後、迅速に免疫測定法に供することができる。生体試料は、結核菌の不活性化処理、又は、分散若しくは可溶化処理による前処理に付されてもよい。

Abstract translation: 通过特异性检测生物样品中结核分枝杆菌复合物特异性分泌蛋白MPT64抗原，结核分枝杆菌感染诊断与以前及时，安全，准确地进行了诊断。 获得识别位于SEQ ID NO：2至4的任何一个氨基酸序列中的MPB64的表位的抗体，特别是单克隆抗体。 因此，提供使用抗体，特别是使用第一抗体和针对MPB64的第二抗体的夹心免疫测定，特别是免疫色谱测定和免疫色谱测试条的免疫测定。 可以将试验样品迅速进行免疫测定而不培养生物样品，或者仅在样品中结核分枝杆菌复合细菌基本上不增殖的时间内培养生物样品后进行免疫测定。 可以通过灭活处理或结核分枝杆菌的分散或增溶处理对生物样品进行预处理。