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    METHOD AND APPARATUS FOR MAGNETIC SEPARATION OF CELLS
    84.
    发明申请
    METHOD AND APPARATUS FOR MAGNETIC SEPARATION OF CELLS 审中-公开
    用于细胞分离的方法和装置

    公开(公告)号：WO2009076560A3

    公开(公告)日：2010-01-07

    申请号：PCT/US2008086486

    申请日：2008-12-11

    Applicant: UNIV LELAND STANFORD JUNIOR ,  TALASAZ AMIRALI HAJHOSSEIN ,  POWELL ASHLEY ANN ,  PEASE FABIAN R ,  JEFFREY STEFANIE S ,  DAVIS RONALD W ,  MINDRINOS MICHAEL

    Inventor： TALASAZ AMIRALI HAJHOSSEIN ,  POWELL ASHLEY ANN ,  PEASE FABIAN R ,  JEFFREY STEFANIE S ,  DAVIS RONALD W ,  MINDRINOS MICHAEL

    IPC: C12N13/00

    CPC classification number: G01N33/54333 ,  B03C1/01 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/26

    Abstract: Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.

    Abstract translation: 这里描述了一种自动化的机器人装置，其以非常高的纯度分离循环肿瘤细胞（CTC）或其他生物结构。 该设备使用强大的磁棒覆盖在可拆卸的塑料袖子。 这些杆穿过血液样品，捕获例如用与磁性响应性颗粒如超顺磁珠连接的抗体标记的癌细胞。 在完成捕获方案后，磁棒经历几轮洗涤，从而除去所有污染的血细胞。 通过从套管中去除磁棒，将捕获的靶细胞释放到最终的捕获溶液中。 此外，由该装置捕获的细胞在捕获后培养时显示出没有降低的活力。 在适合遗传分析的状态下捕获细胞。 还公开了用于单细胞分析的方法。 机器人允许设备以高吞吐量运行。

    LIGAND CONCENTRATING, LIQUID CRYSTAL BIOSENSOR SYSTEM
    88.
    发明申请
    LIGAND CONCENTRATING, LIQUID CRYSTAL BIOSENSOR SYSTEM 审中-公开
    液晶浓缩液晶生物传感器系统

    公开(公告)号：WO2007036905A3

    公开(公告)日：2009-04-16

    申请号：PCT/IB2006053545

    申请日：2006-09-28

    Applicant: UNIV KENT STATE OHIO ,  WOOLVERTON CHRISTOPHER

    Inventor： WOOLVERTON CHRISTOPHER

    IPC: G01N33/53

    CPC classification number: G01N33/5438 ,  B01L3/5027 ,  G01N33/54333

    Abstract: A method for detecting a ligand is provided. Antibodies to a predetermined ligand are attached to substrates. The substrates are superparamagnetic, dyed beads. The beads are exposed to an electromagnetic field to immobilize the beads. The beads are contacted with a sample and the antibodies are allowed to recognize and capture the ligand in the sample. The electromagnetic field is optionally removed. The beads are contacted with a liquid crystalline material and the light transmission properties of the liquid crystalline material are examined for alteration caused by the presence of aggregates of the beads and the ligand. A functional cassette for the detection of ligands comprises a pair of opposed, transparent substrates defining a space therebetween, a pair of transparent electrodes disposed on at least a portion of the opposed substrates on a side facing the opposing substrate and defining a primary detection area, and a secondary detection area adapted to capture aggregates of a ligand and ligand-recognizing antibodies attached to superparamagnetic, dyed beads. The detection areas are in fluid communication with each other.

    Abstract translation: 提供了一种检测配体的方法。 将预定配体的抗体连接到底物上。 底物是超顺磁性染色珠。 珠子暴露于电磁场以固定珠粒。 将小珠与样品接触，并允许抗体识别并捕获样品中的配体。 可选地去除电磁场。 珠子与液晶材料接触，并检查液晶材料的透光性，以改变由珠子和配体的聚集体的存在引起的变化。 用于检测配体的功能盒包括一对在其间限定空间的相对的透明基板，一对透明电极，其设置在面对相对基板的一侧上相对的基板的至少一部分上并且限定初级检测区域， 以及适于捕获附着于超顺磁染色珠粒上的配体和配体识别抗体的聚集体的二次检测区域。 检测区域彼此流体连通。

    MICROELECTRONIC SENSOR DEVICE WITH AN ARRAY OF DETECTION CELLS
    89.
    发明申请
    MICROELECTRONIC SENSOR DEVICE WITH AN ARRAY OF DETECTION CELLS 审中-公开
    具有检测电极阵列的微电子传感器装置

    公开(公告)号：WO2009040712A2

    公开(公告)日：2009-04-02

    申请号：PCT/IB2008/053813

    申请日：2008-09-19

    Applicant: KONINKLIJKE PHILIPS ELECTRONICS N. V. ,  VAN LANKVELT, Petrus, J., W. ,  NIEUWENHUIS, Jeroen, H. ,  VAN ZON, Hans

    Inventor： VAN LANKVELT, Petrus, J., W. ,  NIEUWENHUIS, Jeroen, H. ,  VAN ZON, Hans

    CPC classification number: G01N27/745 ,  G01N15/0656 ,  G01N33/54326 ,  G01N33/54333 ,  G01N2015/0693 ,  G01R33/1269

    Abstract: The invention relates to a microelectronic sensor device with a matrix array of rows (R4, R5) and columns (Cl, C2) of detection cells (10), wherein each detection cell comprises an activation element (30) for transferring target particles (e.g. magnetic beads) into an activated state and a sensor element (20) for detecting activated target particles. According to a preferred embodiment, the activation elements (20) of each row of the matrix as well as the sensor elements (20) of each column of the matrix are connected in series. By activating one row and reading out one column, each detection cell (10) can thus individually be addressed with a limited number of column- and row-address circuits.

    Abstract translation: 本发明涉及具有检测单元（10）的行（R4，R5）和列（C1，C2）的矩阵阵列的微电子传感器装置，其中每个检测单元包括用于转移目标颗粒的活化元件（例如， 磁珠）转换为激活状态，以及用于检测激活的靶颗粒的传感器元件（20）。 根据优选实施例，矩阵的每行的激活元件（20）以及矩阵的每列的传感器元件（20）串联连接。 通过激活一行并读出一列，每个检测单元（10）因此可以用有限数量的列和行地址电路单独寻址。

    METHOD FOR THE PURIFICATION OF AT LEAST ONE TARGET SUBSTANCE THAT IS TO BE IDENTIFIED
    90.
    发明申请
    METHOD FOR THE PURIFICATION OF AT LEAST ONE TARGET SUBSTANCE THAT IS TO BE IDENTIFIED 审中-公开
    法提纯至少一个目标物质

    公开(公告)号：WO2008055671A3

    公开(公告)日：2008-09-18

    申请号：PCT/EP2007009648

    申请日：2007-11-07

    Applicant: PETER JOCHEN

    Inventor： PETER JOCHEN

    IPC: G01N33/543 ,  C12N5/00

    CPC classification number: G01N33/54333 ,  C12N5/0018

    Abstract: Disclosed is a method for purifying at least one target substance that is to be identified and is present or is formed in a cell culture medium when cells are cultivated. In said method, magnetic particles, i.e. beads, to the functionalized surface of which the target substance selectively attaches, are added to the cell culture medium, and the particles to which the target substance is attached are selected out of the cell culture medium by applying a magnetic field. The method is characterized by the following steps: a serum substitute is provided that is obtained from a natural serum and is free or virtually free of low-molecular substances having a maximum mass of 60 kDa, particularly a maximum mass of 10 kDa; the serum substitute is admixed to the cell culture medium which already contains the cells or to which the cells are added; the cells are incubated in the cell culture medium enriched with serum substitute; at least some of the cell culture supernatant formed during the incubation is separated; the cell culture supernatant is filtered by means of an ultrafiltering process so as to obtain a retentate; the beads are supplied in such a way that the functionalized surface of the beads comprises a plurality of dendrimers containing up to 10 branches, i.e. ten generations, the terminal points of the last generation of each dendrimer being modified; the beads and the retentate are admixed to a buffer solution so as to obtain a mixture; the target substances contained in the retentate are incubated and are fixed to the beads; and the magnetic beads are magnetically selected out of the mixture.

    Abstract translation: 用于纯化的方法被描述的目标物质，所述während.einer细胞培养在细胞培养介质中的至少发生或形成，其中细胞培养基中的磁性官能在其表面上的粒子，即所谓的小珠，被添加到其表面的目标物质选择性地 退火并通过施加磁场，有损粒子与目标物质从细胞培养基中进行选择。 该方法的特征在于以下步骤：提供由天然存在的血清回收的，并且不含或几乎不含是低分子量物质，其具有小于或等于60千道尔顿，特别是小于或血清替代物的等于10kDa的质量，具有，混合所述 血清替代品，其中所述细胞包含或加入到其中的细胞中，细胞培养基中温育细胞的含血清替代细胞培养基富集的，分离至少一部分自调节温育细胞培养物上清液中，通过超滤获得过滤细胞培养物上清液 滞留物提供所述珠，使得多个树枝状聚合物的含多至10个分支的小珠，即，官能化表面 十代，其中所述最后一代树枝状聚合物的末端点被修饰的，混合的珠和在缓冲液中渗余物以形成混合物，温育并结合靶物质包含在渗余物到珠子和从磁珠磁性选择 混合物。

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