公开(公告)号：WO2017095917A1

公开(公告)日：2017-06-08

申请号：PCT/US2016/064206

申请日：2016-11-30

Applicant: ILLUMINA, INC.

Inventor： JAMSHIDI, Arash ,  LIN, Yan-you ,  ABSALAN, Farnaz ,  STUART, Sarah ,  CANN, Gordon ,  WU, Yir-Shyuan ,  KHURANA, Tarun ,  FISHER, Jeffrey, S.

IPC: C12Q1/68 ,  G01N1/28 ,  B01L3/00 ,  G01N15/10

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N33/54333 ,  G01N2015/1006 ,  C12Q2535/122

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

Abstract translation: 根据本文的实施例，提供了一种用于捕捉数字微流体系统中感兴趣的细胞的方法，包括利用微滴致动器将样本微滴传送到微孔装置。 微孔装置包括具有多个微孔的基底，所述多个微孔通向微孔装置的微滴操作表面。 样品液滴包括进入微孔的感兴趣的细胞。 该方法将捕获珠引入到微孔中，并且捕获元件被固定在捕获珠上。 该方法利用液滴致动器将细胞裂解试剂液滴传送到微孔设备。 细胞裂解试剂液滴的部分进入微孔，并且在潜伏期期间，导致感兴趣的细胞释放由捕获珠捕获元件捕获的分析物。

公开(公告)号：WO2017029346A1

公开(公告)日：2017-02-23

申请号：PCT/EP2016/069563

申请日：2016-08-18

Applicant: F. HOFFMANN-LA ROCHE AG ,  ROCHE DIAGNOSTICS GMBH ,  ROCHE DIAGNOSTICS OPERATIONS, INC.

Inventor： ZARNT, Toralf ,  HEINDL, Dieter ,  NICHTL, Alfons ,  SICHERL-BIRK, Frank

IPC: G01N33/543

CPC classification number: G01N33/54353 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54393

Abstract: Herein is reported a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein said microparticles are coated with the first partner of a binding pair, the method comprising mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of comprising or comprising the analyte, wherein said second partner of the binding pair is bound to said analyte-specific binding agent via a linker comprising from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles comprising the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.

Abstract translation: 本文报道了一种用于在基于微粒的分析物特异性结合测定中测量分析物的方法，其中所述微粒用结合对的第一个配偶体包被，该方法包括将经包被的微粒，分析物特异性结合剂共轭 涉及结合对的第二个配偶体，以及怀疑包含或包含分析物的样品，其中所述结合对的所述第二个配偶体通过包含12-30个乙二醇单元（PEG）的接头与所述分析物特异性结合剂结合 12至30），从而将分析物通过共轭分析物特异性结合剂与经涂覆的微粒结合，将包含通过结合对结合的分析物和分析物特异性结合剂的微粒与混合物分离，并测量与 微粒。

公开(公告)号：WO2017001705A1

公开(公告)日：2017-01-05

申请号：PCT/EP2016/065731

申请日：2016-07-04

Applicant: UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN

Inventor： KILINC, Devrim ,  LI, Peng ,  LEE, Gil ,  GHANDI, Dhruv ,  RAMPINI, Stefano

IPC: B03C1/24 ,  B03C1/28 ,  G01N27/00

CPC classification number: B03C1/24 ,  B03C1/288 ,  B03C2201/26 ,  G01N27/745 ,  G01N33/54333

Abstract: There is provided system and a separation substrate device for use with an NLM separator in separating and/or detecting at least one target analyte in a sample, the substrate comprising a micromagnet array of a plurality of micromagnets, the micromagnet array comprising a first capture region, a second focussing region, and, a third detection region, the focussing region comprising a converging and/or diverging micromagnet array region. Also provided is a method for separating and detecting at least one target analyte in a sample. The method including: contacting a plurality of magnetic beads with a sample, the magnetic beads functionalized for binding with one or more target analytes in a sample to form aggregates; providing the sample including magnetic beads and aggregates to a separating substrate comprising a micromagnet array of a plurality of micromagnets; transporting the magnetic beads and aggregates relative to the micromagnet array to provide separation and enable detection of the magnetic beads and aggregates; detecting motion of the beads or aggregates of the sample on the array in response to the applied magnetic field and/or detecting beads or aggregates of the sample on the array at a detection region of the array.

Abstract translation: 提供了用于与NLM分离器一起分离和/或检测样品中的至少一种目标分析物的分离衬底装置，所述分离衬底装置包括多个微型磁体的微磁体阵列，所述微磁体阵列包括第一捕获区域 ，第二聚焦区域和第三检测区域，所述聚焦区域包括会聚和/或发散的微电磁阵列区域。 还提供了用于分离和检测样品中至少一种目标分析物的方法。 该方法包括：使多个磁珠与样品接触，所述磁珠被功能化以与样品中的一种或多种目标分析物结合以形成聚集体; 将包括磁珠和聚集体的样品提供到包括多个微型磁体的微磁体阵列的分离衬底; 相对于微磁体阵列运送磁珠和聚集体以提供分离并使得能够检测磁珠和聚集体; 响应于施加的磁场和/或在阵列的检测区域处检测阵列上的样品的珠粒或聚集体来检测样品的珠粒或聚集体在阵列上的运动。

公开(公告)号：WO2016024931A1

公开(公告)日：2016-02-18

申请号：PCT/TR2015/050052

申请日：2015-07-31

Applicant: BOĞAZIÇI ÜNİVERSİTESİ

Inventor： TORUN, Hamdi ,  ERGENEMAN, Olgac ,  PANÉ İ VİDAL, Salvador ,  NELSON, Bradley J.

IPC: G01Q10/04 ,  G01Q60/42

CPC classification number: G01N33/54366 ,  G01N33/54333 ,  G01N33/54346 ,  G01Q10/04 ,  G01Q60/42 ,  G01Q70/12

Abstract: The present invention relates to a biomolecular measurement system (1), which enables to measure the intermolecular forces arising from the interaction between two biomolecules or the intramolecular forces within a single biomolecule by using an atomic force microscope (AFM). In the present invention, the cantilever (2) is moved without using an actuator for moving the cantilever (2). It is moved only when the actuator (4) moves the magnetic nanowire (3) and thus moves the molecule attached to the end of the magnetic nanowire (3). Since the cantilever (2) or a substrate is not moved by another actuator as in the state of the art, fluctuation and disturbance is not created in the liquid containing the biomolecules. Thus, the measurements are made more accurately and with higher resolution. Additionally, by means of the actuator (4), the biomolecules are enabled to be moved upon exertion of magnetic force at any coordinate on x, y and z axes on the nanowire (3), or exertion of torque on two axes.

Abstract translation: 本发明涉及生物分子测量系统（1），其能够通过使用原子力显微镜（AFM）测量由单个生物分子内的两个生物分子或分子内力之间的相互作用产生的分子间力。 在本发明中，悬臂（2）移动而不使用用于移动悬臂（2）的致动器。 只有当致动器（4）移动磁性纳米线（3）并因此移动附着在磁性纳米线（3）的端部上的分子时，它才被移动。 由于如现有技术那样悬臂（2）或基板不被其它致动器移动，所以在含有生物分子的液体中不产生波动和干扰。 因此，更精确地进行测量并且具有更高的分辨率。 另外，通过致动器（4），生物分子能够在纳米线（3）上的x，y和z轴上的任何坐标上施加磁力时移动，或者在两个轴上施加扭矩。

公开(公告)号：WO2016007745A2

公开(公告)日：2016-01-14

申请号：PCT/US2015039747

申请日：2015-07-09

Applicant: ZEPTO LIFE TECHNOLOGY LLC

Inventor： QIU JIAOMING

IPC: G01N33/543 ,  G01R33/09

CPC classification number: G01N33/5434 ,  G01N33/54333 ,  G01R33/093 ,  G01R33/1269

Abstract: Methods, systems and programing for substance detection with a magnetic sensor are presented. In one example, a magnetic sensor having one or more layers is formed on a base for sensing a magnetic field created by magnetic particles present in proximity to the magnetic sensor. A first end of each of a first set of strands is immobilized with respect to the magnetic sensor. A magnetic particle is attached to a second end of each of the first set of strands so that when a material containing a substance is in contact with the base, the substance causes at least some of the first set of strands to break resulting in that the magnetic particle attached to the second end of each of the at least some of the first set of strands is no longer in proximity to the magnetic sensor.

Abstract translation: 介绍了使用磁传感器进行物质检测的方法，系统和编程。 在一个示例中，具有一层或多层的磁传感器形成在基座上，用于感测由磁传感器附近存在的磁性颗粒产生的磁场。 第一组股线的第一端相对于磁性传感器被固定。 磁性颗粒附着到第一组股线的第二端，使得当含有物质的材料与基底接触时，该物质导致第一组股线中的至少一些断裂，导致 附着到第一组股线中的至少一些中的每一个的第二端的磁性颗粒不再靠近磁性传感器。

公开(公告)号：WO2015193474A1

公开(公告)日：2015-12-23

申请号：PCT/EP2015/063817

申请日：2015-06-19

Applicant: SPINCHIP DIAGNOSTICS AS

Inventor： BORCH, Stig, Morten ,  HAMSUND, Torgeir ,  HOLTLUND, Jostein, Geir ,  GULLIKSEN, Anja

IPC: G01N33/543

CPC classification number: G01N1/10 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2400/0409 ,  B01L2400/0487 ,  B03D3/00 ,  G01N33/54313 ,  G01N33/54333 ,  G01N33/54366 ,  G01N2001/1006 ,  G01N2035/0449

Abstract: The present invention provides a method of separating beads in a fluidic chip comprising an internal fluid circuit through which various reactants, in which at least one of the reactants are beads, may be moved by use of centrifugal force, the method comprises the steps of: providing at least a first set of beads (8a) having a density ml and a second set of beads (8b) having a density m2 in a section (7, 15, 18) of the fluid circuit, the section comprising at least a first outlet (16, 13, 17); providing a first liquid medium in the section, the liquid medium having a density d3, such that ml

Abstract translation: 本发明提供了一种在流体芯片中分离珠粒的方法，该流体碎片包括内部流体回路，通过该内部流体回路可以通过使用离心力使至少一个反应物为珠粒的各种反应物移动，该方法包括以下步骤： 提供具有密度ml的至少第一组珠子（8a）和在所述流体回路的部分（7,15,18）中具有密度m2的第二组珠粒（8b），所述部分包括至少第一 出口（16,13,17）; 在该部分中提供第一液体介质，液体介质具有密度d3，使得ml

公开(公告)号：WO2015143277A1

公开(公告)日：2015-09-24

申请号：PCT/US2015/021678

申请日：2015-03-20

Applicant: WELLSTAT DIAGNOSTICS, LLC

Inventor： MILLER, Jeffrey, Allan ,  JEFFRIES, Eddie, Phillip

IPC: C07K16/18 ,  G01N33/53 ,  G01N33/58

CPC classification number: G01N33/6893 ,  C07K16/18 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/72 ,  G01N33/54333 ,  G01N2333/4742 ,  G01N2510/00 ,  G01N2800/085 ,  G01N2800/7033

Abstract: Disclosed herein are antibodies having binding specificity to the amino acid sequences Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1 ) and Thr Val Glu Val Asp (SEQ ID NO:14), and methods of detecting cell death in a sample, comprising contacting the sample with a first antibody specific for a C-terminal amino acid sequence Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1 ) or Thr Val Glu Val Asp (SEQ ID NO:14) of a CK18 protein fragment having a C-terminal amino acid sequence of Val Glu Val Asp (SEQ ID NO:2) and a second antibody that specifically binds an epitope that is present in both full-length CK18 and the CK18 protein fragment, and that does not overlap with SEQ ID NO:1 or SEQ ID NO:14, under conditions such that the CK1 8 protein fragment present in the sample specifically binds to the first antibody and the second antibody, wherein one of the antibodies is bound to a solid support and the other antibody is bound to a detection moiety capable of producing a signal; optionally removing any unbound or excess material; and detecting the signal from the detection moiety, wherein the signal is positively correlated with the presence of the CK18 protein fragment in the sample.

Abstract translation: 本文公开了对氨基酸序列Ala Ser Ser Gly Leu Thr Val Glu Val Asp（SEQ ID NO：1）和Thr Val Glu Val Asp（SEQ ID NO：14）具有结合特异性的抗体，以及检测细胞死亡的方法 包括使样品与C末端氨基酸序列Ala Ser Ser Gly Leu Thr Val Glu Val Asp（SEQ ID NO：1）或Thr Val Glu Val Asp（SEQ ID NO：14）特异的第一抗体接触， 的具有Val Glu Val Asp（SEQ ID NO：2）的C末端氨基酸序列的CK18蛋白片段和特异性结合存在于全长CK18和CK18蛋白片段中的表位的第二抗体，以及 其不与SEQ ID NO：1或SEQ ID NO：14重叠，使得样品中存在的CK1 8蛋白片段特异性结合第一抗体和第二抗体，其中一种抗体结合于 固体支持物和另一抗体结合到能产生信号的检测部分; 任选地去除任何未结合或多余的材料; 并检测来自检测部分的信号，其中该信号与样品中CK18蛋白片段的存在呈正相关。

公开(公告)号：WO2015113047A2

公开(公告)日：2015-07-30

申请号：PCT/US2015/013097

申请日：2015-01-27

Applicant: BREVITEST TECHNOLOGIES, LLC

Inventor： CHATTERJEE, Dev Kumar ,  VARADHACHARY, Atul ,  LINBECK, Leo III

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: G01N33/54333 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/021 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0858 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2400/043 ,  G01N21/78 ,  G01N2333/908

Abstract: Disclosed herein are systems and methods for using the controlled movement of magnetic particles using controlled magnetic fields in a fluidic device containing separated fluidic regions to detect analytes in solution by immunoassay, such as an enzyme-linked immunosorbant assay (ELISA) for various medical and scientific applications. In order to achieve sequential exposure to the different chemical environments required in an immunoassay, magnetic particles are driven through fluid-containing chambers separated by air-gaps that may take the form of air bubbles or small open-air separations, for example. Externally controlled magnets coupled to actuators draw the flow of magnetic particles through air-liquid interfaces produced by microfluidic surface tension at the air-gap, washing the particles.

Abstract translation: 这里公开的是用于利用受控磁场在包含分离的流体区域的流体装置中利用受控磁场来控制磁性颗粒的受控运动以通过免疫测定检测溶液中的分析物的系统和方法，诸如酶联免疫吸附测定 （ELISA）用于各种医学和科学应用。 为了实现顺序暴露于免疫测定中所需的不同化学环境，例如，磁性粒子被驱动通过由可能采取气泡或小型露天分离形式的气隙分开的含流体室。 与致动器耦合的外部控制磁体吸引磁性粒子流经由气隙处的微流体表面张力产生的气 - 液界面，清洗粒子。

公开(公告)号：WO2015054287A3

公开(公告)日：2015-06-11

申请号：PCT/US2014059537

申请日：2014-10-07

Applicant: UNIV UTAH RES FOUND

Inventor： SANT HIMANSHU JAYANT ,  GALE BRUCE K ,  JAYAMOHAN HARIKRISHNAN ,  LAMBERT CHRISTOPHER J ,  MINSON JOHN

IPC: C12Q1/00 ,  C12M1/34 ,  C12M3/00

CPC classification number: G01N33/56916 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N33/54333 ,  G01N33/5438 ,  G01N2333/245 ,  G01N2458/10 ,  C12Q2563/113 ,  C12Q2563/149 ,  C12Q2565/607

Abstract: Methods of electrochemically detecting or quantifying an analyte by coupling a plurality of redox-active agents (e.g., guanine-rich oligonucleotides) to the analyte are disclosed. More particularly, this application discloses affinity-based methods for isolating one or more analytes from a sample and subsequently detecting or determining the concentration of the one or more analytes. Detecting or determining the concentration of one or more analytes may involve measuring the extent of oxidation of guanine nucleobases that have been or are coupled to the analyte.

Abstract translation: 公开了通过将多种氧化还原活性剂（例如，富含鸟嘌呤的寡核苷酸）偶联至分析物来电化学检测或定量分析物的方法。 更具体地，本申请公开了用于从样品中分离一种或多种分析物并随后检测或确定一种或多种分析物的浓度的基于亲和力的方法。 检测或确定一种或多种分析物的浓度可涉及测量已经或偶联于分析物的鸟嘌呤核苷碱基的氧化程度。

公开(公告)号：WO2014164933A1

公开(公告)日：2014-10-09

申请号：PCT/US2014/023821

申请日：2014-03-11

Applicant: RUUBIX, INC.

Inventor： KHATTAK, Ayub ,  SEVER, Clinton

IPC: G01N35/08 ,  G01N33/48

CPC classification number: B01L3/502715 ,  B01L3/5027 ,  B01L3/502723 ,  B01L3/502738 ,  B01L3/5029 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/087 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/023 ,  B01L2300/025 ,  B01L2300/04 ,  B01L2300/044 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/12 ,  B01L2300/161 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0677 ,  B01L2400/0683 ,  B05D3/002 ,  B05D2518/00 ,  C12Q1/6825 ,  F16K99/0032 ,  F16K99/0036 ,  F16K2099/0084 ,  G01N1/02 ,  G01N21/78 ,  G01N27/28 ,  G01N27/3271 ,  G01N27/3272 ,  G01N27/3273 ,  G01N33/54306 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/581 ,  G01N35/00029 ,  G01N35/0098 ,  G01N35/08 ,  G01N35/1095 ,  G01N2001/027 ,  G01N2001/028 ,  G01N2035/00277 ,  G01N2035/00564 ,  G01N2458/30 ,  H04M1/72527 ,  H04M1/7253 ,  Y10T137/1797

Abstract: Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Abstract translation: 本文描述了用于检测收集的样品中的感兴趣分子的装置，系统和方法。 在某些实施例中，公开了自包含的样品分析系统，其包括可重复使用的读取器部件，一次性盒部件和一次性样品收集部件。 在一些实施例中，读取器组件与远程计算设备通信，用于测试协议和测试结果的数字传输。 在各种公开的实施方案中，系统，组分和方法被配置为鉴定特定核酸，蛋白质或其它感兴趣的分析物的存在，不存在和/或数量，例如，为了测试一种 或更多的病原体或污染物。