公开(公告)号：WO2016086015A1

公开(公告)日：2016-06-02

申请号：PCT/US2015/062478

申请日：2015-11-24

Applicant: UNIVERSITY OF ROCHESTER ,  FASAN, Rudi

Inventor： FASAN, Rudi

IPC: C12P17/18

CPC classification number: C12P13/001 ,  C07K14/805 ,  C12P5/002 ,  C12P9/00 ,  C12P11/00

Abstract: Methods are provided for carrying out carbene transfer transformations such as olefin cyclopropanation reactions, carbene heteroatom-H insertion reactions (heteroatom = N, S, Si), sigmatropic rearrangement reactions, and aldehyde olefination reactions with high efficiency and selectivity by using a novel class of myoglobin-based biocatalysts. These methods are useful for the synthesis of a variety of organic compounds which contain one or more new carbon-carbon or carbon-heteroatom (N, S, or Si) bond. The methods can be applied for conducting these transformations in vitro (i.e., using the biocatalyst in isolated form) and in vivo (i.e., using the biocatalyst in a whole cell system).

Abstract translation: 提供了用于通过使用新颖的类别的方法进行碳烯转移转化如烯烃环丙烷化反应，卡宾杂原子-H插入反应（杂原子= N，S，Si），信号重排反应和醛烯化反应，以高效率和选择性 基于肌红蛋白的生物催化剂。 这些方法可用于合成含有一个或多个新的碳 - 碳或碳 - 杂原子（N，S或Si）键的各种有机化合物。 该方法可用于体外进行这些转化（即，以分离形式使用生物催化剂）和体内（即，在整个细胞系统中使用生物催化剂）。

公开(公告)号：WO2013130711A1

公开(公告)日：2013-09-06

申请号：PCT/US2013/028164

申请日：2013-02-28

Applicant: THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK

Inventor： PADALINO, David J. ,  CALANCIE, Blair

IPC: A61B5/0478 ,  A61B5/0408 ,  A61B5/0492

CPC classification number: A61B5/04001 ,  A61B5/0478 ,  A61B5/0492 ,  A61B5/6833 ,  A61B5/6849 ,  A61B17/3494

Abstract: A needle electrode is provided incorporating a protective flap and optionally a retraction chamber. By providing an impenetrable barrier covering the area of skin over the needle's tip, the protective flap protects against needle sticks that can occur when the tip of a needle electrode is pushed back out through the skin. An adhesive layer on the skin-side of the protective flap secures the needle in place without the use of a separate piece of adhesive tape. Other types of needles can incorporate flaps for protection or for securing the needle to the patient.

Abstract translation: 提供一种针电极，其结合有保护翼片和任选的收缩腔室。 通过提供覆盖针尖端的皮肤区域的不可渗透的屏障，保护片防止当针电极的尖端被推出皮肤时可能发生的针刺。 保护膜的皮肤侧上的粘合剂层将针固定到位，而不使用单独的粘合带。 其他类型的针可以包括用于保护的护翼或将针固定到患者身上。

公开(公告)号：WO2013090536A1

公开(公告)日：2013-06-20

申请号：PCT/US2012/069442

申请日：2012-12-13

Applicant: EPHESUS TECHNOLOGIES, LLC

Inventor： NOLAN, Christopher ,  CASPER, Joseph ,  WITKOWSKI, Joseph ,  HULL, Jonathan

IPC: F21V29/00 ,  F21V5/00 ,  F21V23/00 ,  F21Y101/02 ,  F21Y105/00

CPC classification number: F21V23/009 ,  F21K9/00 ,  F21K9/60 ,  F21K9/69 ,  F21S2/00 ,  F21V3/049 ,  F21V5/007 ,  F21V15/013 ,  F21V17/12 ,  F21V21/08 ,  F21V21/14 ,  F21V21/30 ,  F21V23/005 ,  F21V23/0464 ,  F21V23/0471 ,  F21V29/70 ,  F21V29/74 ,  F21V29/75 ,  F21V29/773 ,  F21V29/89 ,  F21V31/005 ,  F21Y2101/00 ,  F21Y2105/10 ,  F21Y2115/10 ,  H05B33/0803 ,  H05B33/0827 ,  H05B33/0845 ,  H05B33/089 ,  H05B37/0281

Abstract: A low-cost, efficient, high intensity LED luminaire (HILL) assembly for use indoors or outdoors in wet, damp, or dry environments. In various embodiments, the HILL assembly can be powered by a universal AC or a DC electrical supply and can operate in a temperature range from about -40°C to about +85 °C. The HILL assembly can include a lens element comprising one or more concavo-convex lenses; an interchangeable LED module comprising a plurality of LEDs positioned in a LED array; and a heatsink housing containing a power supply for the LEDs. The HILL assembly can optionally comprise a circuit board for the LED array that employs thermal via technology, a lens with a frosted lip for attenuating the light source as seen from an angle, and/or a sensor for sensing an environmental parameter of interest. Driver circuitry and the LEDs are preferably mounted directly on a common circuit board.

Abstract translation: 低成本，高效，高强度的LED灯具（HILL）组件，可在室内或室外在潮湿，潮湿或干燥的环境中使用。 在各种实施例中，HILL组件可以由通用AC或DC电源供电，并且可以在约-40℃至约+85℃的温度范围内操作。 HILL组件可以包括包括一个或多个凹凸透镜的透镜元件; 可互换的LED模块，包括位于LED阵列中的多个LED; 以及包含用于LED的电源的散热器外壳。 HILL组件可以可选地包括用于LED阵列的电路板，其采用热通孔技术，具有用于从角度来看衰减光源的磨砂唇部的透镜和/或感测感兴趣的环境参数的传感器。 驱动器电路和LED优选地直接安装在公共电路板上。

公开(公告)号：WO2012109586A2

公开(公告)日：2012-08-16

申请号：PCT/US2012/024723

申请日：2012-02-10

Applicant: UNIVERSITY OF ROCHESTER ,  FASAN, Rudi ,  ZHANG, Kaidong

Inventor： FASAN, Rudi ,  ZHANG, Kaidong

IPC: C12Q1/26 ,  C12N9/02 ,  C07C43/03

CPC classification number: C12Q1/26 ,  C12N15/102 ,  C12N15/1093 ,  C40B30/08 ,  G01N2333/90241 ,  G01N2333/90245

Abstract: Methods and systems for evaluating and predicting the reactivity of natural and engineered monooxygenase enzymes are provided. Methods are provided for acquiring a functional profile (fingerprint) of monooxygenases that encode information regarding the active site configuration of such monooxygenases. Methods are also provided for carrying out analysis of a monooxygenase fingerprint, to formulate predictions regarding the reactivity properties (e.g., substrate reactivity, chemo-, regio, and stereoselectivity properties) of the fingerprinted monooxygenases.

Abstract translation: 提供了用于评估和预测天然和工程化单加氧酶的反应性的方法和系统。 提供了用于获取编码关于这种单加氧酶的活性位点构型的信息的单加氧酶的功能谱（指纹图谱）的方法。 还提供了用于进行单加氧酶指纹分析的方法，以制定关于指纹图谱的单加氧酶的反应性（例如底物反应性，化学，区域和立体选择性）的预测。

公开(公告)号：WO2011014674A3

公开(公告)日：2011-02-03

申请号：PCT/US2010/043743

申请日：2010-07-29

Applicant: CORNELL UNIVERSITY ,  SHULER, Michael L. ,  SUNG, Jong Hwan

Inventor： SHULER, Michael L. ,  SUNG, Jong Hwan

IPC: C12M3/02 ,  G01N33/50 ,  G01N33/15 ,  C12Q1/02

Abstract: A microfluidic device for culturing cells, termed a microscale cell culture analog (µCCA), is provided. The microfluidic device allows multiple cell or tissue types to be cultured in a physiologically relevant environment, facilitates high-throughput operation and can be used for drug discovery. The microfluidic device uses gravity-induced fluidic flow, eliminating the need for a pump and preventing formation of air bubbles. Reciprocating motion between a pair of connected reservoirs is used to effect the gravity-induced flow in microfluidic channels. Bacterial contamination is reduced and high throughput enabled by eliminating a pump. The microfluidic device integrates a pharmacokinetic - pharmacodynamic( PK-PD) model to enable PK-PD analyses on-chip. This combined in vitro/ in silico system enables prediction of drug toxicity in a more realistic manner than conventional in vitro systems.

公开(公告)号：WO2010044932A9

公开(公告)日：2010-04-22

申请号：PCT/US2009/050157

申请日：2009-07-09

Applicant: CORNELL UNIVERSITY ,  MANNION, John, T. ,  CRAIGHEAD, Harold, C.

Inventor： MANNION, John, T. ,  CRAIGHEAD, Harold, C.

IPC: G01N27/00 ,  G01N27/02 ,  G01N27/414 ,  B82B3/00 ,  G01N33/48 ,  C12Q1/68

Abstract: An electrical detector is provided that comprises a nanofluidic channel with an integrated nanoscale charge sensor. The charge sensor can be an unfunctionalized nanowire, nanotube, transistor or capacitor and can be of carbon, silicon, carbon/silicon or other semiconducting material. The nanofluidic channel depth is on the order of the Debye screening length. Methods are also provided for detecting charged molecules or biological or chemical species with the electrical detector. Charged molecules or species in solution are driven through the nanofluidic channel of the electrical detector and contact the charge sensor, thereby producing a detectable signal. Methods are also provided for detecting a local solution potential of interest. A solution flowing through the nanofluidic channel of the electrical detector contacts the charge sensor, thereby producing a detectable local solution potential signal.

公开(公告)号：WO2009102629A3

公开(公告)日：2009-08-20

申请号：PCT/US2009/033350

申请日：2009-02-06

Applicant: BALAN BIOMEDICAL, INC. ,  PYSZCZEK, Michael, F.

Inventor： PYSZCZEK, Michael, F.

IPC: H01M10/38 ,  H01M4/04

Abstract: The invention provides a thin film solid state (TFSS) battery that can conform to the surface of an apparatus having a complex, three-dimensional surface. The invention also provides methods for constructing the thin film solid state battery by forming components directly onto a substrate of a complex three-dimensional shape. The resulting thin film solid state battery can be used to power electronics associated with a variety of devices such as medical devices.

公开(公告)号：WO2009049268A1

公开(公告)日：2009-04-16

申请号：PCT/US2008/079659

申请日：2008-10-10

Applicant: KIONIX, INC. ,  ZHOU, Peng ,  YOUNG, Lincoln C. ,  ROSWECH, Todd ,  SPIZZ, Gwendolyn ,  CHEN, Zongyuan ,  THOMAS, Benjamin W. ,  LEE, Travis

Inventor： ZHOU, Peng ,  YOUNG, Lincoln C. ,  ROSWECH, Todd ,  SPIZZ, Gwendolyn ,  CHEN, Zongyuan ,  THOMAS, Benjamin W. ,  LEE, Travis

IPC: B01L3/00

CPC classification number: B01F13/0059 ,  B01F5/0683 ,  B01F5/0688 ,  B01F11/0074 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1816 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2300/185 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/084

Abstract: A microf luidic device for analyzing a sample of interest is provided. The tnicrof luidic device can comprise a microfluidic device body, wherein the microf luidic device body comprises a sample preparation area (101), a nucleic acid amplification area (102), a nucleic acid analysis area (103), and a network of fluid channels. Each of the sample preparation area (101), the nucleic acid amplification area (102) and the nucleic acid analysis area (103) are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysisof a biological sample.

Abstract translation: 提供了一种用于分析感兴趣样品的微流控装置。 微流体装置可以包括微流体装置主体，其中微流体装置主体包括样品制备区域（101），核酸扩增区域（102），核酸分析区域（103）和流体通道网络 。 每个样品制备区域（101），核酸扩增区域（102）和核酸分析区域（103）通过至少一个流体通道流体地互连到另外两个区域中的至少一个区域。 使用微流体装置，样品制备可以与生物活性分子的扩增相结合，并且可以提供合适的生物样品用于分析和/或检测目的分子。 与用于生物样品的制备和分析的较大规模的设备相比，所提供的小型装置和方法更容易，更快，更便宜，并且同样有效。

公开(公告)号：WO2009042538A2

公开(公告)日：2009-04-02

申请号：PCT/US2008/077182

申请日：2008-09-22

Applicant: CORNELL UNIVERSITY ,  CHANG, Yung-fu

Inventor： CHANG, Yung-fu

IPC: C07K14/20

CPC classification number: C07K14/20 ,  A61K39/0225 ,  A61K2039/53 ,  Y02A50/48

Abstract: Leptospira outer membrane proteins (OMPs) LP1454, LP1118, LP1939, MCEII, CADF-like1, CADF-like2, CADF-like3, Lp0022, Lp1499, Lp4337, Lp328 or L21 are provided. The OMPS can be used as tools for developing effective vaccines or diagnostic methods for leptospirosis. Expression vectors for the OMP genes are further provided. The antigenic properties of the Leptospira OMPs can be used to create, manufacture or improve vaccines. Vaccines, including but not limited to DNA vaccines, recombinant vaccines, and T-cell epitope vaccines based on the foregoing OMPs are also provided. Methods for producing such vaccines are also provided. Also provided are methods for using Leptospira OMP genes, proteins and antibodies for therapeutic treatment and serological diagnosis technique s.

Abstract translation: 钩端螺旋体外膜蛋白（OMP）LP1454，LP1118，LP1939，MCEII，CADF样1，CADF样2，CADF样3，Lp0022，Lp1499，Lp4337，Lp328或 L21提供。 OMPS可用作开发钩端螺旋体病有效疫苗或诊断方法的工具。 进一步提供了OMP基因的表达载体。 钩端螺旋体OMPs的抗原特性可用于制造，制造或改进疫苗。 还提供了疫苗，包括但不限于基于前述OMP的DNA疫苗，重组疫苗和T细胞表位疫苗。 还提供了生产这种疫苗的方法。 还提供了使用钩端螺旋体OMP基因，蛋白质和抗体进行治疗性治疗和血清学诊断技术的方法。

公开(公告)号：WO2014070673A1

公开(公告)日：2014-05-08

申请号：PCT/US2013/067118

申请日：2013-10-28

Applicant: UNIVERSITY OF ROCHESTER

Inventor： FASAN, Rudi

IPC: C12N9/02 ,  C12N15/53 ,  A61K31/335

CPC classification number: C07D493/18 ,  C07D491/18 ,  C12N9/0042 ,  C12N9/0071 ,  C12P17/181 ,  C12Y106/02004 ,  C12Y114/00

Abstract: Derivatives of the antimalarial agent artemisinin, compositions comprising the derivatives, methods for preparing the derivatives, and their uses in pharmaceutical compositions intended for the treatment of parasitic infections are provided. Methods are provided for the production of artemisinin derivatives via functionalization of positions C7 and C6a, and optionally, in conjunction with modifications at positions C10 and C9, via chemoenzymatic methods. Recombinant cytochrome P450 polypeptides are also provided for use in the methods. The artemisinin derivatives can be used for the treatment of malaria and other parasitic infections, alone or in combination with other antiparasitic drugs.

Abstract translation: 提供了抗疟剂青蒿素的衍生物，包含衍生物的组合物，制备衍生物的方法及其用于治疗寄生虫感染的药物组合物中的用途。 提供了通过位置C7和C6a官能化生产青蒿素衍生物的方法，以及任选地通过化学酶法与C10和C9位置的修饰相结合。 还提供重组细胞色素P450多肽用于该方法。 青蒿素衍生物可用于单独或与其它抗寄生虫药物组合治疗疟疾和其他寄生虫感染。