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31 条记录 (耗时 0.041 秒)

    METHOD AND APPARATUS FOR CONTROLLING POSITION OF A LASER OF A MALDI MASS SPECTROMETER
    3.
    发明申请
    METHOD AND APPARATUS FOR CONTROLLING POSITION OF A LASER OF A MALDI MASS SPECTROMETER 审中-公开
    用于控制激光质谱仪的位置的方法和装置

    公开(公告)号：WO2004083811A2

    公开(公告)日：2004-09-30

    申请号：PCT/US2004/006822

    申请日：2004-03-05

    申请人： ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, INC. ,  REILLY, James, P. ,  BORAAS, Kirk, S. ,  CHRISTIAN, Noah, P.

    发明人： REILLY, James, P. ,  BORAAS, Kirk, S. ,  CHRISTIAN, Noah, P.

    IPC分类号： G01N

    CPC分类号： H01J49/164 ,  H01J49/0004

    摘要： A MALDI mass spectrometer directs a laser shot onto a MALDI sample to generate a sample spectrum which is analyzed to determine if the sample spectrum meets a predetermined criteria. If so, subsequent laser shots are directed to predetermined locations on the MALDI sample. In essence, if the analysis of a previous laser shot indicates that a “sweet spot” of the MALDI sample has be located, subsequent laser shots may be directed to areas proximate to the previous shot thereby allowing the sweet spot to be thoroughly sampled. A method of operating a MALDI mass spectrometer is also disclosed.

    摘要翻译： MALDI质谱仪将激光射击引导到MALDI样品上以产生样品光谱，其被分析以确定样品光谱是否符合预定标准。 如果是这样，随后的激光照射被引导到MALDI样品上的预定位置。 实质上，如果先前的激光镜头的分析表明已经定位了MALDI样品的“最佳点”，则随后的激光照射可以被定向到接近先前镜头的区域，从而允许将甜点彻底地采样。 还公开了一种操作MALDI质谱仪的方法。

    MALDI MASS SPECTROMETER HAVING A LASER STEERING ASSEMBLY AND METHOD OPERATING THE SAME
    4.
    发明申请
    MALDI MASS SPECTROMETER HAVING A LASER STEERING ASSEMBLY AND METHOD OPERATING THE SAME 审中-公开
    具有激光转向装置的MALDI质谱仪及其操作方法

    公开(公告)号：WO2004083810A2

    公开(公告)日：2004-09-30

    申请号：PCT/US2004/006649

    申请日：2004-03-05

    申请人： ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, INC. ,  REILLY, James, P. ,  BORAAS, Kirk, S. ,  CHRISTIAN, Noah, P.

    发明人： REILLY, James, P. ,  BORAAS, Kirk, S. ,  CHRISTIAN, Noah, P.

    IPC分类号： G01N

    CPC分类号： H01J49/164

    摘要： A MALDI mass spectrometer has a laser steering assembly operable to steer or otherwise direct movement of a laser focus over the MALDI sample being tested. Such a laser steering assembly may include a mirror array having a pair of independently controlled mirrors. The first of such a pair of mirrors is operable to move the laser focus along the X-axis of the MALDI sample, whereas the second of such a pair of mirrors is operable to move the laser focus along the Y-axis of the MALDI sample. A method of operating a MALDI mass spectrometer is also disclosed.

    摘要翻译： MALDI质谱仪具有可操作以引导或以其它方式直接将激光焦点移动到所测试的MALDI样品上的激光转向组件。 这种激光转向组件可以包括具有一对独立控制的反射镜的反射镜阵列。 这样的一对反射镜中的第一个可操作以沿着MALDI样品的X轴移动激光焦点，而这样的一对反射镜中的第二个可操作来沿着MALDI样品的Y轴移动激光焦点 。 还公开了一种操作MALDI质谱仪的方法。

    METHOD OF PREPARATION OF OLIGOSACCHARIDES
    5.
    发明申请
    METHOD OF PREPARATION OF OLIGOSACCHARIDES 审中-公开
    寡糖的制备方法

    公开(公告)号：WO2004045502A2

    公开(公告)日：2004-06-03

    申请号：PCT/US2003/034088

    申请日：2003-10-24

    申请人： ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, INC. ,  HUANG, Yunping ,  KONSE, Tomonori ,  MECHREF, Yehia, S. ,  NOVOTNY, Milos, V.

    发明人： HUANG, Yunping ,  KONSE, Tomonori ,  MECHREF, Yehia, S. ,  NOVOTNY, Milos, V.

    IPC分类号： A61K

    CPC分类号： C07H1/08

    摘要： The invention provides a method of cleaving an 0-linked oligosaccharide from a glycoprotein. The method comprises the steps of contacting a composition comprising a glycoprotein, wherein the glycoprotein comprises 0-linked oligosaccharides, with a solution comprising a borane-ammonia complex to form a mixture comprising the glycoprotein and the borane-ammonia complex, incubating the mixture for a period of time sufficient to cleave the linked oligosaccharides from the glycoprotein, and forming a mixture comprising oligosaccharide alditol products and deglycosylated protein by-products.

    摘要翻译： 本发明提供了从糖蛋白切割O-连接的寡糖的方法。 该方法包括以下步骤：使包含糖蛋白的组合物（其中糖蛋白包含O-连接的寡糖）与包含硼烷 - 氨络合物的溶液形成包含糖蛋白和硼烷 - 氨络合物的混合物，将该混合物温育 足以从糖蛋白切割连接的寡糖的时间，以及形成包含寡糖糖醇产物和去糖基化蛋白副产物的混合物。

    GLYCOPROTEIN CLEAVAGE PROTOCOL FOR OLIGOSACCHARIDE ANALYSIS
    6.
    发明申请
    GLYCOPROTEIN CLEAVAGE PROTOCOL FOR OLIGOSACCHARIDE ANALYSIS 审中-公开
    GLYCOPROTEIN CLEAVAGE PROLOCOL FOR OLIGOSACCHARIDE ANALYSIS

    公开(公告)号：WO2004045501A2

    公开(公告)日：2004-06-03

    申请号：PCT/US2003/033888

    申请日：2003-10-24

    申请人： ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, INC. ,  HUANG, Yunping ,  MECHREF, Yehia, S. ,  NOVOTNY, Milos, V.

    发明人： HUANG, Yunping ,  MECHREF, Yehia, S. ,  NOVOTNY, Milos, V.

    IPC分类号： A61K

    CPC分类号： C07H1/08

    摘要： An ammonium-based ß-elimination cleavage of linked oligosaccharides from glycoproteins is described. The method enables the isolation of glycoprotein-derived oligosaccharides having a reducing end which enables subsequent derivatization for chromatographic and/or mass spectral analysis. The described glycoprotein cleavage protocol enables structural investigations using low microgram quantities of glycoproteins.

    摘要翻译： 描述了从糖蛋白中连接的寡糖的铵基ß-消除切割。 该方法能够分离具有还原末端的糖蛋白衍生的寡糖，其能够随后进行色谱和/或质谱分析的衍生化。 所描述的糖蛋白切割方案能够使用低微量的糖蛋白进行结构调查。

    METHOD AND APPARATUS FOR SEPARATING IONS IN TIME AS FUNCTIONS OF PRESELECTED ION MOBILITY AND ION MASS
    8.
    发明申请
    METHOD AND APPARATUS FOR SEPARATING IONS IN TIME AS FUNCTIONS OF PRESELECTED ION MOBILITY AND ION MASS 审中-公开
    时间分离离子的方法和装置作为离子交换和离子质量的函数

    公开(公告)号：WO2002086946A1

    公开(公告)日：2002-10-31

    申请号：PCT/US2002/012390

    申请日：2002-04-19

    申请人： ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, INC. ,  CLEMMER, David, E.

    发明人： CLEMMER, David, E.

    IPC分类号： H01J49/40

    CPC分类号： G01N27/622 ,  H01J49/004 ,  H01J49/38

    摘要： An ion separation instrument includes an ion source coupled to at least a first ion mobility spectrometer having an ion outlet coupled to a mass spectrometer only ions defining a preselected ion mobility range. In one embodiment, the ion mobility spectrometer is provided with electronically controllable inlet and outlet gates as a function of ion drift time to thereby allow passage therethrough only of ions defining a mobility within the preselected ion mobility reange. In another embodiment, an ion trap is disposed between the ion mobility spectrometer and mass spectrometer and is controlled in such a manner so as to collect a plurality of ions defining a mobility within the preselected ion mobility range prior to injection of such ions into the mass spectrometer. In yet another embodiment, an ion inlet of the ion trap may be electronically controlled relative to operation of the ion mobility spectrometer as a function of ion drift time to thereby allow passage therein only of ions defining a mobility within the preselected ionmobility range. The mass spectrometer is preferably a Fourier Transform Ion Cyclotron Resonance mass spectrometer, and the resulting ion separation instrument may further include therein various combinations of ion fragmentation, ion mass filtering, ion trap, charge neutralization and/or mass reaction instrumentation.

    摘要翻译： 离子分离仪器包括耦合到至少第一离子迁移谱仪的离子源，其具有仅耦合到质谱仪的离子出口，仅限于限定预选离子迁移率范围的离子。 在一个实施方案中，离子迁移谱仪具有作为离子漂移时间的函数的电子可控的入口和出口浇口，从而允许仅限定在预选离子迁移率范围内限定迁移率的离子。 在另一个实施方案中，将离子阱设置在离子迁移谱仪和质谱仪之间，并以这样的方式进行控制，以便在将这样的离子注入质量之前收集限定预选离子迁移率范围内的迁移率的多个离子 光谱仪。 在另一个实施方案中，离子阱的离子入口可以相对于离子迁移谱仪的操作而电子控制，作为离子漂移时间的函数，从而允许仅限定在预选离子迁移范围内限定迁移率的离子。 质谱仪优选为傅里叶变换离子回旋共振质谱仪，所得到的离子分离仪器还可以包括离子碎片化，离子质量过滤，离子阱，电荷中和和/或质量反应仪器的各种组合。

    METHOD FOR IDENTIFYING PEPTIDES BY MASS SPECTROMETRY
    10.
    发明申请
    METHOD FOR IDENTIFYING PEPTIDES BY MASS SPECTROMETRY 审中-公开

    公开(公告)号：WO2004067551A3

    公开(公告)日：2004-08-12

    申请号：PCT/US2003/041819

    申请日：2003-12-31

    申请人： ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, INC. ,  REILLY, James, P. ,  BEARDSLEY, Richard, L.

    发明人： REILLY, James, P. ,  BEARDSLEY, Richard, L.

    IPC分类号： H01J49/00

    摘要： A method for identifying the N-terminal amino acid residue of a peptide or protein using mass spectrometry is described. Amidination of the N-terminus of the peptide or protein results in the efficient cleavage of the N-terminal amino acid by ion fragmentation during mass spectral analysis. The observed mass of the peptide ion and that of the Y n-1 ion can be used to calculate the mass of and thus identify the N-terminal amino acid. Methods are also described for identifying proteins using component peptide mass data and the identity of the N-terminal amino acid residues of component peptides.