公开(公告)号：WO2014132063A1

公开(公告)日：2014-09-04

申请号：PCT/GB2014/050583

申请日：2014-02-27

Applicant: ALCYOMICS LTD ,  UNIVERSITY OF NEWCASTLE UPON TYNE

Inventor： LOVAT, Penny ,  HILL, David ,  DICKINSON, Anne ,  AHMED, Shaheda

IPC: C12N5/071 ,  A61B5/00 ,  G01N33/50

CPC classification number: G01N33/94 ,  A61B5/411 ,  A61B5/445 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/54 ,  G01N33/5044 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5064 ,  G01N33/5082 ,  G01N2800/24 ,  G09B23/28

Abstract: A three dimensional (3-D) model comprising a scaffold and autologous skin cells, the invention also provides methods of predicting immunogenicity and hypersensitivity or allergic or adverse immune reactions to potential therapeutic compounds, biologies, cosmetics and chemical sensitizers using the 3-D model of skin cells. The methods provide an in vitro assay employing autologous blood derived cells in the 3-D skin equivalent model and is of particular utility in the identification and prediction of skin sensitizers and in particular agents that may cause allergic contact dermatitis. The assay of the present invention provides inter alia methods of screening library compounds for sensitizing activity, identifying optimal therapeutics, especially but not exclusively, monoclonal antibodies and kits therefor.

Abstract translation: 包括支架和自体皮肤细胞的三维（3-D）模型，本发明还提供使用3-D模型预测免疫原性和对潜在的治疗化合物，生物制品，化妆品和化学敏化剂的过敏或过敏或不良免疫反应的方法 的皮肤细胞。 该方法提供了在3-D皮肤等效模型中使用自体血液来源的细胞的体外测定，并且在鉴定和预测皮肤敏化剂，特别是可能引起过敏性接触性皮炎的试剂中尤其有用。 本发明的测定特别提供了筛选文库化合物以增强活性，鉴定最佳治疗剂的方法，特别但非排他地，单克隆抗体及其试剂盒。

公开(公告)号：WO2012069838A1

公开(公告)日：2012-05-31

申请号：PCT/GB2011/052312

申请日：2011-11-24

Applicant: ALCYOMICS LTD ,  DICKINSON, Anne

Inventor： DICKINSON, Anne

IPC: G01N33/50

CPC classification number: G01N33/505 ,  C12Q1/6809 ,  G01N33/6854

Abstract: This present invention provides a method of predicting immunogenicity and hypersensitivity or allergic reactions to chemical compounds, therapeutics, cosmetics and other chemical compositions. The method uses an in vitroassay employing autologous bloodderived cells and an autologous cultured skin biopsy and is of particular utility in the identification and prediction of skin sensitizers that cause allergic contact dermatitis.

Abstract translation: 本发明提供一种预测化学化合物，治疗剂，化妆品和其它化学组合物的免疫原性和过敏反应或过敏反应的方法。 该方法使用采用自体造血细胞和自体培养的皮肤活检的体外测定，并且在鉴定和预测引起过敏性接触性皮炎的皮肤敏化剂中尤其有用。