公开(公告)号：WO1991009136A1

公开(公告)日：1991-06-27

申请号：PCT/ES1990000046

申请日：1990-12-12

Applicant: CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS ,  GUISAN SEIJAS, José, M.; ,  FERNANDEZ-LAFUENTE, Roberto; ,  ALVARO CAMPOS, Gregorio; ,  BLANCO MARTIN, Rosa, Mª; ,  MOLINA ROSELL, Cristina;

Inventor： CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS

IPC: C12P35/04

CPC classification number: C12P35/04 ,  C12P37/04

Abstract: Synthesis of semi-synthetic monobactamic or beta -lactamic antibiotics by using derivatives stabilized by various methods of penicillin G acylase from various microbial sources according to a thermodynamically controlled strategy in monophase water/cosolvent organic apolar systems, wherein the concentration of the cosolvent varies between 30 % and 90 %, the temperature between -10 DEG C and 50 DEG C, the pH between 4.5 and 8.5, with concentrations of the antibiotic nucleus between 0.5 and 875 mM and acyl donor between 0.2 mM and 1 M, with a relationship antibiotic ring/activated or free acyl donor, using a buffer between 0 and 1 M. Application to the pharmaceutical industry.

Abstract translation: 根据单相水/助溶剂有机非极性体系中的热力学控制策略，通过使用通过各种微生物来源的各种青霉素G酰基转移酶稳定的衍生物合成半合成单体或β-乳酸抗生素，其中助溶剂的浓度在30 ％和90％，温度在-10℃和50℃之间，pH在4.5和8.5之间，抗生素核的浓度在0.5和875mM之间，酰基供体在0.2mM和1M之间，具有关系抗生素环 /活化或游离酰基供体，使用0和1 M之间的缓冲液。应用于制药工业。