公开(公告)号：WO1994001454A1

公开(公告)日：1994-01-20

申请号：PCT/ES1993000057

申请日：1993-07-02

Applicant: CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS ,  GUISAN, José, M. ,  MOLINA-ROSELL, Cristina ,  FERNANDEZ-LAFUENTE, Roberto ,  CEINOS, Mª del Carmen

Inventor： CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS

IPC: C07K03/18

CPC classification number: B01J20/3085 ,  B01J20/28016 ,  B01J20/28047 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3255 ,  B01J20/3257 ,  B01J20/3263

Abstract: The method is essentially based on the reaction of ethylenediamine with inert supports, containing a controlled single layer of similar aldehyde groups which are moderately separated from the support surface and on the following reduction by using borohydride of the activated supports in order to eliminate the reactive additional groups which could otherwise cause secondary indesired interactions. Applications to biotechnology, specially for the purification of proteins and for the preparation of fixed protein derivatives.

Abstract translation: 该方法基本上是基于乙二胺与惰性载体的反应，其含有与支持体表面中度分离的受控单层相似的醛基，并且通过使用活化的载体的硼氢化物进行以下还原，以消除反应性附加 否则可能会导致次要的不相关的互动。 应用于生物技术，特别是用于纯化蛋白质和用于制备固定蛋白质衍生物。

公开(公告)号：WO1991009136A1

公开(公告)日：1991-06-27

申请号：PCT/ES1990000046

申请日：1990-12-12

Applicant: CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS ,  GUISAN SEIJAS, José, M.; ,  FERNANDEZ-LAFUENTE, Roberto; ,  ALVARO CAMPOS, Gregorio; ,  BLANCO MARTIN, Rosa, Mª; ,  MOLINA ROSELL, Cristina;

Inventor： CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS

IPC: C12P35/04

CPC classification number: C12P35/04 ,  C12P37/04

Abstract: Synthesis of semi-synthetic monobactamic or beta -lactamic antibiotics by using derivatives stabilized by various methods of penicillin G acylase from various microbial sources according to a thermodynamically controlled strategy in monophase water/cosolvent organic apolar systems, wherein the concentration of the cosolvent varies between 30 % and 90 %, the temperature between -10 DEG C and 50 DEG C, the pH between 4.5 and 8.5, with concentrations of the antibiotic nucleus between 0.5 and 875 mM and acyl donor between 0.2 mM and 1 M, with a relationship antibiotic ring/activated or free acyl donor, using a buffer between 0 and 1 M. Application to the pharmaceutical industry.

Abstract translation: 根据单相水/助溶剂有机非极性体系中的热力学控制策略，通过使用通过各种微生物来源的各种青霉素G酰基转移酶稳定的衍生物合成半合成单体或β-乳酸抗生素，其中助溶剂的浓度在30 ％和90％，温度在-10℃和50℃之间，pH在4.5和8.5之间，抗生素核的浓度在0.5和875mM之间，酰基供体在0.2mM和1M之间，具有关系抗生素环 /活化或游离酰基供体，使用0和1 M之间的缓冲液。应用于制药工业。