公开(公告)号：WO2005069980A2

公开(公告)日：2005-08-04

申请号：PCT/US2005/000211

申请日：2005-01-05

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  BALAGADDE, Frederick ,  HANSEN, Carl, L. ,  KARTALOV, Emil ,  QUAKE, Stephen, R.

Inventor： BALAGADDE, Frederick ,  HANSEN, Carl, L. ,  KARTALOV, Emil ,  QUAKE, Stephen, R.

IPC: B01L3/00 ,  C12M1/00 ,  C12M1/113 ,  C12M1/18 ,  C12M3/00 ,  C12P19/04 ,  C12P19/34 ,  C12Q1/02 ,  C12Q1/68

CPC classification number: C12P19/04 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2300/163 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12M23/16 ,  C12M23/34 ,  C12M39/00 ,  C12P1/00

Abstract: A chemostat that includes a growth chamber having a plurality of compartments, where each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.

Abstract translation: 一种恒温器，其包括具有多个室的生长室，其中每个室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，可以防止在恒化器的生长室中形成生物膜的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。

公开(公告)号：WO2005069980A3

公开(公告)日：2005-09-29

申请号：PCT/US2005000211

申请日：2005-01-05

Applicant: CALIFORNIA INST OF TECHN ,  BALAGADDE FREDERICK ,  HANSEN CARL L ,  KARTALOV EMIL ,  QUAKE STEPHEN R

Inventor： BALAGADDE FREDERICK ,  HANSEN CARL L ,  KARTALOV EMIL ,  QUAKE STEPHEN R

IPC: B01L3/00 ,  C12M1/00 ,  C12M1/113 ,  C12M1/18 ,  C12M3/00 ,  C12P19/04 ,  C12P19/34 ,  C12Q1/02 ,  C12Q1/68

CPC classification number: C12P19/04 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2300/163 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12M23/16 ,  C12M23/34 ,  C12M39/00 ,  C12P1/00

Abstract: A chemostat (Figure 1) that includes a growth chamber having a plurality of compartments, where each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber (s), and an output port to remove fluids from the growth chamber. Also, presented is a method to prevent biofilm formation in a growth chamber of the chemostat. The method may include the step of adding a lytic agent to an isolated portion of the growth chamber and re-uniting the isolated portion with the rest of the growth chamber.

Abstract translation: 一种恒温器（图1），其包括具有多个室的生长室，其中每个室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 还提出了防止生长膜在恒化器生长室中形成的方法。 该方法可以包括将溶解剂添加到生长室的分离部分并将分离的部分与生长室的其余部分重新结合的步骤。