公开(公告)号：WO2005087934A2

公开(公告)日：2005-09-22

申请号：PCT/DK2005/000160

申请日：2005-03-09

Applicant: CARLSBERG A/S ,  BREDDAM, Klaus ,  OLSEN, Ole ,  SKADHAUGE, Birgitte ,  LOK, Finn ,  KNUDSEN, Søren ,  BECH, Lene, Mølskov

Inventor： BREDDAM, Klaus ,  OLSEN, Ole ,  SKADHAUGE, Birgitte ,  LOK, Finn ,  KNUDSEN, Søren ,  BECH, Lene, Mølskov

IPC: C12N15/82

CPC classification number: C12C1/18 ,  A01H5/10 ,  C12C5/004 ,  C12C7/04 ,  C12C7/047 ,  C12C11/003 ,  C12C12/00 ,  C12N9/0069 ,  C12N15/8242 ,  C12N15/8243

Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.

Abstract translation: 根据本发明，提供了无效LOX-1大麦及其生产的植物产品，例如通过使用缺乏合成脂肪酸转化酶脂氧合酶-1的大麦籽粒制备的麦芽。 所述酶涉及与将亚油酸转化为9-氢过氧十八碳二烯酸（脂氧合酶途径代谢物）相关的主要活性，其通过进一步的酶促或自发反应可能导致反式-2-壬烯醛的出现。 本发明使酿造商能够生产没有可检测的反式-2-壬烯醛特异性异味的啤酒，甚至在长时间储存​​饮料之后。

公开(公告)号：WO2010075860A2

公开(公告)日：2010-07-08

申请号：PCT/DK2009/050355

申请日：2009-12-28

Applicant: CARLSBERG BREWERIES A/S ,  HEINEKEN SUPPLY CHAIN B.V. ,  SKADHAUGE, Birgitte ,  LOK, Finn ,  BREDDAM, Klaus ,  OLSEN, Ole ,  BECH, Lene Mølskov ,  KNUDSEN, Søren

Inventor： SKADHAUGE, Birgitte ,  LOK, Finn ,  BREDDAM, Klaus ,  OLSEN, Ole ,  BECH, Lene Mølskov ,  KNUDSEN, Søren

IPC: A01H5/10 ,  C12C1/18 ,  C12C7/00 ,  C12C12/00 ,  C12N15/01

CPC classification number: A01H5/10 ,  C12C1/18 ,  C12C12/00 ,  C12C2200/01

Abstract: According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid,respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which - through further enzymatic or spontaneous reactions - may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale,T2N-specific off-flavors, even after prolonged storage of the beverage.

Abstract translation: 根据本发明，提供了具有功能性脂氧合酶（LOX）-1和LOX-2酶的总损失的大麦及其生产的植物产品，例如通过使用在合成脂肪酸 - 双氧合中缺陷的大麦仁制备的麦芽 酶LOX-1和LOX-2。 所述酶分别涉及与亚油酸双加氧相关的9-和13-氢过氧十八碳二烯酸的主要活性。 9-氢过氧十八碳二烯酸代表LOX途径代谢物，其通过进一步的酶促或自发反应可能导致反式-2-壬醛（T2N）的出现。 本发明使酿造商能够生产具有不明显水平的陈年T2N特异性异味的啤酒，即使在饮料长期储存之后。

公开(公告)号：WO2010063288A2

公开(公告)日：2010-06-10

申请号：PCT/DK2009/050315

申请日：2009-12-01

Applicant: Carlsberg Breweries A/S ,  Heineken Supply Chain B.V. ,  KNUDSEN, Søren ,  HAMBRAEUS, Gustav ,  BECH, Lene Mølskov ,  SØRENSEN, Steen, Bech ,  SKADHAUGE, Birgitte ,  BREDDAM, Klaus ,  OLSEN, Ole

Inventor： KNUDSEN, Søren ,  HAMBRAEUS, Gustav ,  BECH, Lene Mølskov ,  SØRENSEN, Steen, Bech ,  SKADHAUGE, Birgitte ,  BREDDAM, Klaus ,  OLSEN, Ole

IPC: A01H5/10 ,  C12C1/18 ,  C12C7/00 ,  C12C12/00 ,  C12N15/01

CPC classification number: C12N9/1007 ,  C12C1/18 ,  C12C12/00 ,  C12C2200/01 ,  C12N15/8243

Abstract: According to the invention, there is provided barley-derived beverages characterized by notably reduced levels of both dimethyl sulfide (DMS) and/or its precursor S-methyl-L- methionine (SMM), or lacking said compounds. In addition, the invention relates to methods for producing the above-mentioned beverages - and also to barley plants useful in the preparation of such beverages, as well as other plant products prepared from said plants. Utilization of the invention clears the way for making improved production procedures of beverages with improved taste profiles, and promises also for notable reductions in the thermal energy input for production of beer.

Abstract translation: 根据本发明，提供了大麦衍生的饮料，其特征在于二甲硫醚（DMS）和/或其前体S-甲基-L-甲硫氨酸（SMM）的水平显着降低，或缺乏所述化合物。 此外，本发明涉及生产上述饮料的方法以及用于制备这种饮料的大麦植物以及从所述植物制备的其它植物产品。 本发明的利用清除了具有改进的味道特征的饮料改进生产程序的方法，并且还有助于显着降低生产啤酒的热能输入。

公开(公告)号：WO2011150933A3

公开(公告)日：2011-12-08

申请号：PCT/DK2011/050186

申请日：2011-05-31

Applicant: CARLSBERG BREWERIES A/S ,  HEINEKEN SUPPLY CHAIN B.V. ,  KNUDSEN, Søren ,  RIIS, Preben ,  SKADHAUGE, Birgitte ,  BECH, Lene, Mølskov ,  OLSEN, Ole

Inventor： KNUDSEN, Søren ,  RIIS, Preben ,  SKADHAUGE, Birgitte ,  BECH, Lene, Mølskov ,  OLSEN, Ole

IPC: A01H5/10 ,  C12C1/18 ,  C12C7/00 ,  C12C12/00 ,  C12N9/10 ,  C12N15/82

Abstract: Barley based beverages are produced in large quantities, employing highly energy consuming methods, for example in the malting and brewhouse facilities for kiln drying and wort boiling operations, respectively. The present invention relates to energy saving methods for preparing barley based beverages, as well as to barley plants useful in such methods. In particular, the invention describes barley plants with combined traits of null-lipoxygenase-1 (null-LOX-1), null-lipoxygenase-2 (null-LOX-2) and null-S-adenosylmethionine:methionine S-methyltransferase in one plant, which is particularly useful for energy saving methods to prepare barley based beverages, such as beer.