公开(公告)号：WO2008064220A3

公开(公告)日：2008-11-06

申请号：PCT/US2007085232

申请日：2007-11-20

Applicant: CELLOPTIC INC ,  BROOKER GARY

Inventor： BROOKER GARY

IPC: G02B21/06

CPC classification number: G02B21/0076

Abstract: A wide field microscope includes a stage configured to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source configured to produce excitation light having a single photon energy less than an absorption energy required for single photon excitation of said fluorescent material, A beam expansion unit is optically coupled to the light source and configured to expand the excitation light with reduced pulse spreading characteristics, and an infinity corrected objective optically coupled to the expansion unit and configured to focus the excitation light onto the specimen such that multi-photon excitation of the fluorescent material simultaneously occurs over a predetermined area of the specimen. A focus lens is configured to focus emission light emitted from said predetermined area of the specimen onto at least two pixels of an image detector simultaneously.

Abstract translation: 一种宽视场显微镜，包括：台，用于保持具有荧光物质的试样;以及多光子激励光源，用于产生单光子能量小于所述荧光物质的单光子激发所需吸收能量的激发光 ，光束扩展单元光学地耦合到光源并且被配置为以减小的脉冲扩展特性来扩展激发光，以及无限远校正物镜，其光学地耦合到扩展单元并且被配置为将激发光聚焦到样本上， 荧光材料的光子激发同时发生在样本的预定区域上。 聚焦透镜被配置为将从所述样本的所述预定区域发射的发射光同时聚焦到图像检测器的至少两个像素上。

公开(公告)号：WO2005116717A2

公开(公告)日：2005-12-08

申请号：PCT/US2005/017096

申请日：2005-05-17

Applicant: BROOKER, Gary

Inventor： BROOKER, Gary

IPC: G02B21/00

CPC classification number: G02B21/0076

Abstract: According to one aspect of the invention, a wide-field microscope includes a stage configured to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source configured to produce a substantially parallel beam of excitation light having a single photon energy less than an absorption energy required for single photon excitation of the fluorescent material. An infinity corrected objective is optically coupled to the multi-photon excitation light source and configured to focus the substantially parallel beam of excitation light onto the specimen such that multi-photon excitation of the fluorescent material simultaneously occurs over a predetermined area of the specimen. A focus lens is configured to focus emission light emitted from the predetermined area of the specimen onto at least two pixels of an image detector simultaneously. A focus lens is configured to focus emission light emitted from the predetermined area of the specimen onto an image plane, such that the image plane can be viewed through a binocular eyepiece or an imaging array detector.

Abstract translation: 根据本发明的一个方面，一种宽视场显微镜包括一个被配置为将其中具有荧光材料的样本保持在其中的台，以及多光子激发光源，被配置为产生基本平行的具有单个光子能量的激发光束 小于荧光材料的单光子激发所需的吸收能量。 无穷远校正的物镜与多光子激发光源光学耦合，并被配置为将基本上平行的激发光束聚焦到样本上，使得荧光材料的多光子激发同时发生在样本的预定区域上。 聚焦透镜被配置为将从样本的预定区域发射的发射光同时聚焦到图像检测器的至少两个像素。 聚焦透镜被配置为将从样本的预定区域发射的发射光聚焦到图像平面上，使得可以通过双目目镜或成像阵列检测器观察图像平面。

公开(公告)号：WO2016010860A1

公开(公告)日：2016-01-21

申请号：PCT/US2015/040024

申请日：2015-07-10

Applicant: BROOKER, Gary

Inventor： BROOKER, Gary ,  SIEGEL, Nisan

IPC: G01B9/021

CPC classification number: G03H1/0005 ,  G02B21/361 ,  G02B21/367 ,  G02B27/58 ,  G03H1/041 ,  G03H1/0443 ,  G03H1/0486 ,  G03H1/06 ,  G03H1/28 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/0458 ,  G03H2210/33 ,  G03H2222/31 ,  G03H2222/34 ,  G03H2222/36 ,  G03H2223/12 ,  G03H2223/17 ,  G03H2223/52 ,  G03H2240/56

Abstract: A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

Abstract translation: 用于产生三维物体的单个平面的全息图的系统和方法包括从所述物体的单个平面引出电磁辐射的电磁辐射组件以及将所引出的电磁辐射引向全息图形成组件的组件。 全息图形成组件产生由图像捕获组件记录的全息图，然后进一步处理以产生无固有全息伪像的最大分辨率图像。

公开(公告)号：WO2017156425A1

公开(公告)日：2017-09-14

申请号：PCT/US2017/021847

申请日：2017-03-10

Applicant: BROOKER, Gary

Inventor： BROOKER, Gary ,  SIEGEL, Nisan

IPC: G03H1/04 ,  G03H1/08 ,  G03H1/16 ,  G02B5/30

Abstract: Techniques to improve image quality in holography utilizing lenses made from materials with non-quantized anisotropic electromagnetic properties, such as birefringent materials, to advantageously split an incoming beam of light into two coincident beams with different focal lengths that interfere with one another and thus create holograms free of electro-optical or pixelated devices are disclosed. The use of thin birefringent lenses is introduced. Corresponding systems, methods and apparatuses are described.

Abstract translation: 利用由具有非量化各向异性电磁特性的材料（例如双折射材料）制成的透镜来改善全息术中图像质量的技术有利地将入射光束分成具有不同焦距的两个重合光束， 公开了彼此干涉并由此产生没有电光或像素化装置的全息图。 引入了薄双折射透镜的使用。 描述了相应的系统，方法和设备。

公开(公告)号：WO2008064220A2

公开(公告)日：2008-05-29

申请号：PCT/US2007/085232

申请日：2007-11-20

Applicant: CELLOPTIC, INC. ,  BROOKER, Gary

Inventor： BROOKER, Gary

IPC: G02B13/14 ,  G21K5/04

CPC classification number: G02B21/0076

Abstract: A wide field microscope includes a stage configured to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source configured to produce excitation light having a single photon energy less than an absorption energy required for single photon excitation of said fluorescent material, A beam expansion unit is optically coupled to the light source and configured to expand the excitation light with reduced pulse spreading characteristics, and an infinity corrected objective optically coupled to the expansion unit and configured to focus the excitation light onto the specimen such that multi-photon excitation of the fluorescent material simultaneously occurs over a predetermined area of the specimen. A focus lens is configured to focus emission light emitted from said predetermined area of the specimen onto at least two pixels of an image detector simultaneously.

Abstract translation: 宽场显微镜包括配置成保持其中具有荧光材料的样本的台和配置为产生具有小于所述荧光材料的单光子激发所需的吸收能量的单光子能量的激发光的多光子激发光源 光束扩展单元光学耦合到光源并且被配置为以减小的脉冲扩展特性扩展激发光;以及无穷远校正对象，光学耦合到扩展单元，并且被配置为将激发光聚焦到样本上， 荧光材料的光子激发同时发生在样品的预定区域上。 焦点透镜被配置为将从样本的所述预定区域发射的发射光同时聚焦到图像检测器的至少两个像素。

公开(公告)号：WO2008061259A3

公开(公告)日：2008-05-22

申请号：PCT/US2007/085094

申请日：2007-11-19

Applicant: CELLOPTIC, INC. ,  ROSEN, Joseph ,  BROOKER, Gary

Inventor： ROSEN, Joseph ,  BROOKER, Gary

IPC: G03H1/04

Abstract: An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.

公开(公告)号：WO2008010790A1

公开(公告)日：2008-01-24

申请号：PCT/US2006/027727

申请日：2006-07-18

Applicant: CELLOPTIC, INC. ,  ROSEN, Joseph ,  BROOKER, Gary

Inventor： ROSEN, Joseph ,  BROOKER, Gary

IPC: G03H1/04

CPC classification number: G03H1/06 ,  G02B5/32 ,  G03H1/0005 ,  G03H1/0244 ,  G03H1/0443 ,  G03H1/08 ,  G03H5/00 ,  G03H2001/0033 ,  G03H2001/0066 ,  G03H2001/0447 ,  G03H2001/0458 ,  G03H2222/24 ,  G03H2223/12 ,  G03H2223/13 ,  G03H2225/33 ,  G03H2240/13 ,  G03H2240/24

Abstract: An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.

Abstract translation: 用于产生物体的全息图的装置和方法包括电磁辐射组件，该电磁辐射组件被配置为从物体接收诸如光的接收电磁辐射。 电磁辐射组件还被配置为衍射所接收的电磁辐射并且传输衍射的电磁辐射。 图像捕捉组件被配置成捕捉衍射的电磁辐射的图像并且从捕捉的图像产生对象的全息图。

公开(公告)号：WO2017193005A1

公开(公告)日：2017-11-09

申请号：PCT/US2017/031319

申请日：2017-05-05

Applicant: BROOKER, Gary

Inventor： BROOKER, Gary ,  SIEGEL, Nisan

IPC: G03H1/04 ,  G03H1/02 ,  G03H1/06 ,  G03H1/08

CPC classification number: G03H1/06 ,  G03H1/041 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/0216 ,  G03H2001/0447 ,  G03H2001/0452 ,  G03H2222/24 ,  G03H2223/17 ,  G03H2223/20

Abstract: Techniques to improve image quality in holography utilizing lenses made from materials with non-quantized anisotropic electromagnetic properties, such as birefringent materials, to advantageously split an incoming beam of light into two coincident beams with different focal lengths that interfere with one another and thus create holograms free of electro-optical or pixelated devices are disclosed for microscopy and other applications. The use of thin birefringent lenses and single crystal alpha-BBO lenses are introduced. Corresponding systems, methods and apparatuses are described.

Abstract translation: 利用由具有非量化各向异性电磁特性的材料（例如双折射材料）制成的透镜来改善全息术中图像质量的技术有利地将入射光束分成具有不同焦距的两个重合光束， 公开了用于显微镜和其他应用的彼此干涉且因此产生不含电光或像素化装置的全息图。 介绍了使用薄双折射透镜和单晶α-BBO透镜。 描述了相应的系统，方法和设备。

公开(公告)号：WO2015168384A3

公开(公告)日：2016-03-24

申请号：PCT/US2015028477

申请日：2015-04-30

Applicant: BROOKER GARY

Inventor： BROOKER GARY ,  BROOKER GARY ,  SIEGEL NISAN

IPC: G03H1/08 ,  G03H1/04

CPC classification number: G03H1/041 ,  G02B1/08 ,  G02B5/3016 ,  G02B5/3083 ,  G02B27/283 ,  G03H1/0443 ,  G03H1/06 ,  G03H2001/005 ,  G03H2001/0216 ,  G03H2001/0447 ,  G03H2222/31 ,  G03H2223/17 ,  G03H2223/20

Abstract: The inventors have discovered a method to improve image quality in holography and, for the first time, utilize lenses made from birefringent materials to advantageously split an incoming beam of either coherent or incoherent light into two coincident beams with different focal lengths that interfere with one another and thus create holograms free of electro-optical or pixelated devices. This discovery has many advantages over current methods to create holograms in which many components, including multiple lenses, other electro-optical devices, and/or beam paths are necessary to create holograms. The current invention provides a purely optical holographic process which has better performance and holographic simplicity, in addition to being able to miniaturize holographic processes more than is currently possible in state of the art holography systems.

Abstract translation: 本发明人已经发现了一种提高全息图像质量的方法，并且首次利用由双折射材料制成的透镜，以有利地将相干或非相干光的入射光束分成具有彼此干扰的不同焦距的两个重合光束 并因此产生没有电光或像素化装置的全息图。 这个发现与现有的创建全息图的方法相比具有许多优点，其中包括多个透镜，其他电光装置和/或光束路径在内的许多部件需要用于创建全息图。 本发明提供了纯光学全息方法，其具有更好的性能和全息简化，此外，除了能够使全息技术在现有技术的全息照相系统中可能的情况下，全息方法能够小型化。

公开(公告)号：WO2005116717A3

公开(公告)日：2006-10-05

申请号：PCT/US2005017096

申请日：2005-05-17

Applicant: BROOKER GARY

Inventor： BROOKER GARY

IPC: G02B21/00

CPC classification number: G02B21/0076

Abstract: A wide-field microscope includes a stage (50) to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source (10) to produce a substantially parallel beam of excitation light (25) having a single photon energy less than an absorption energy required for single photon excitation of the fluorescent material.

Abstract translation: 宽场显微镜包括用于保持其中具有荧光材料的样本的阶段（50）和多光子激发光源（10），以产生具有单个光子能量的基本上平行的激发光束（25） 比荧光材料的单光子激发所需的吸收能量。