公开(公告)号：WO2019110415A1

公开(公告)日：2019-06-13

申请号：PCT/EP2018/082989

申请日：2018-11-29

Applicant: EUROPEAN MOLECULAR BIOLOGY LABORATORY

Inventor： FALK, Henning ,  HUFNAGEL, Lars ,  AULEHLA, Alexander

IPC: G02B21/00 ,  G01N21/64 ,  G02B21/16 ,  G02B21/36 ,  G01N21/13 ,  G02B21/26 ,  G02B21/34

CPC classification number: G02B21/367 ,  G01N21/13 ,  G01N2021/135 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34

Abstract: A sample holder comprises one or more elongated sample tubes. The one or more elongated sample tubes are adapted for accommodating a plurality of samples to be imaged at an imaging position. The imaging position is defined by at least one illumination objective lens and at least one detection objective lens of a microscope. A microscope is disclosed, comprising at least one illumination objective lens and at least one detection objective lens, which define an imaging position. The microscope further comprises a sample holder for holding a plurality of samples. The sample holder is moveable with respect to the imaging position.A method for imaging a plurality of samples by means of the microscope is disclosed.

公开(公告)号：WO2018182526A1

公开(公告)日：2018-10-04

申请号：PCT/SG2018/050155

申请日：2018-03-29

Applicant: NANYANG TECHNOLOGICAL UNIVERSITY

Inventor： LIU, Quan ,  TIAN, Yao

IPC: G01N21/64 ,  G02B27/00 ,  G02B21/00 ,  G02B5/00 ,  G01J3/44 ,  G02B3/00

CPC classification number: G01N21/6458 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0216 ,  G01J3/0229 ,  G01J3/2803 ,  G01J3/44 ,  G01N2021/6463 ,  G02B5/001 ,  G02B21/006 ,  G02B21/0076 ,  G02B21/367

Abstract: An apparatus (100) for analysing a specimen (102) is disclosed. The apparatus comprises an optical device (104) having reflective or refractive surfaces arranged at a first angle (101) relative to a first axis (103) of the optical device to reflect or refract optical beams emitted from the specimen, the optical beams optically reflected or refracted by the reflective or refractive surfaces to be projected as a distribution of optical points on a plane (105) orthogonal or arranged at a second angle (122) relative to a second axis (107) of the optical device; and an imaging device (106) to process the projected distribution of optical points into at least one image corresponding to a cross-sectional view of a portion of the specimen. A corresponding method for analysing the specimen using the apparatus is also disclosed.

公开(公告)号：WO2018045014A1

公开(公告)日：2018-03-08

申请号：PCT/US2017/049349

申请日：2017-08-30

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： PENG, Leilei ,  LI, Yu ,  ZHANG, Han

IPC: H04N5/225 ,  G01N21/64 ,  G02B21/36 ,  G02B27/58

CPC classification number: G02B21/0076 ,  G01N21/6458 ,  G02B21/0032 ,  G02B21/0068 ,  G02B21/008 ,  G02B21/26 ,  G02B21/33 ,  G02B21/367 ,  G02B27/58

Abstract: A superresolution STED-NSEVI apparatus having an epifluorescence architecture utilizing a 2D structured STED pattern having a N.A. less than a N.A. of the microscope objective and no surface plasmon resonance (SPR) effects. A superresolution STED-NSEVI imaging method using a fully deterministic imaging processing method, in which a pre- calibrated set of parameters are used to process all image data.

Abstract translation: 具有表面荧光结构的超分辨STED-NSEVI装置利用了具有小于显微镜物镜的N.A.的N.A.并且没有表面等离子共振（SPR）效应的2D结构化STED图案。 使用完全确定性成像处理方法的超分辨率STED-NSEVI成像方法，其中使用预先校准的一组参数来处理所有图像数据。

公开(公告)号：WO2018007469A2

公开(公告)日：2018-01-11

申请号：PCT/EP2017/066833

申请日：2017-07-05

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： KNEBEL, Werner ,  FAHRBACH, Florian

IPC: G02B21/00 ,  G02B21/36 ,  G02B27/58

CPC classification number: G02B21/0032 ,  G02B21/0056 ,  G02B21/0076 ,  G02B21/367 ,  G02B27/58

Abstract: Die Erfindung betrifft ein Verfahren zum Untersuchen einer Probe sowie eine Vorrichtung zum Ausführen eines solchen Verfahrens. Die Erfindung betrifft außerdem eine Vorrichtung, die zum Untersuchen einer Probe ausgebildet ist und die wenigstens eine Lichtquelle zum Erzeugen eines Beleuchtungslichtbündels (1), das wenigstens eine zur Fluoreszenzanregung der Probe geeignete Wellenlänge aufweist, und zum Erzeugen eines Abregungs- oder Schaltlichtbündels (6), das wenigstens eine zur Abregung der Probe geeignete Wellenlänge aufweist, beinhaltet und die wenigstens ein Objektiv zum Fokussieren des Beleuchtungslichtbündels (1) und des Abregungs- oder Schaltlichtbündels (6) beinhaltet. Die Vorrichtung zeichnet sich dadurch aus, dass das Beleuchtungslichtbündel (1) und das Abregungs- oder Schaltlichtbündel (6) derart geführt sind, dass eine zu untersuchende Probe in einer Probenebene entlang einer Probenlinie mit dem sich entlang der Probenlinie ausbreitenden Beleuchtungslichtbündel (1) beleuchtet ist und das Abregungs- oder Schaltlichtbündel (6) in der Probenebene wenigstens teilweise räumlich mit dem Beleuchtungslichtbündel (1) überlappt, und dass eine Detektionsoptik vorhanden ist, die ein Detektionsobjektiv beinhaltet und die von dem mittels des Beleuchtungslichtbündels (1) entlang der Probenlinie beleuchteten Probenbereich ausgehendes Detektionslicht in eine Detektionsebene abbildet, in der ein Detektor angeordnet ist, der den Teil des von der Probenebene ausgehenden Fluoreszenzlichts als Detektionslicht detektiert, der aus einem ersten Unterbereich (19) des Überlappungsbereichs stammt, in dem die Wahrscheinlichkeit einer Wechselwirkung der Probenmoleküle mit dem Abregungs- oder Schaltlichtbündel (6) größer als 90%, insbesondere größer 95%, ganz insbesondere größer 99%, ist, und/oder aus einem zweiten Unterbereich (20) stammt, der von dem ersten Unterbereich (19) wenigstens abschnittweise umgeben ist und/oder in dem das Abregungs- oder Schaltlichtbündel (6) eine Nullstelle aufweist, während der Detektor gleichzeitig das von außerhalb des ersten und zweiten Unterbereichs (19, 20) stammende Fluoreszenzlicht wenigstens teilweise ausgeblendet und nicht detektiert.

Abstract translation: 本发明涉及用于检查样品的方法和用于执行这种方法的设备。 本发明涉及AU ROAD此外，其被设计用于测定样品和至少一个光源，用于产生Beleuchtungslichtb导航用途ndels（1），包括至少适于样品波长BEAR长度的荧光激发装置具有，和用于产生Abregungs-的装置 或Schaltlichtb导航用途ndels（6）包括至少一个装置，适于包含和至少一个透镜，用于聚焦Beleuchtungslichtb导航用途包括ndels（6）将样品波长BEAR长度的去激励; ndels（1）和Abregungs-或Schaltlichtb导航用途。 该装置的特征在于：所述Beleuchtungslichtb捆绑（1）和Abregungs-或Schaltlichtb BUNCH（6）这样的实测值导航用途是引线，在一个样品平面随着光波沿着样品线传播的样品线的测定的样品 Beleuchtungslichtb捆绑（1）点亮，Abregungs-或Schaltlichtb BUNCH（6）在试样平面至少部分地 - [R AUML;空间上与Beleuchtungslichtb BUNCH（1）导航用途重叠，并且其提供了一种检测光学系统，其包括检测目标 并且由Beleuchtungslichtb导航用途的装置传出照亮ndels（1）沿着在其中设置用于检测光从样品平面荧光灯发出的作为检测光，所述部分的检测器的检测平面上的采样线样本区域检测光反映了（从第一子区域 19）重叠区域的概率 它的Probenmolek导航用途文件与Abregungs-或Schaltlichtb一堆的相互作用（6）GR＆ouml;道路他大于90％，特别是GR＆ouml;道路他95％，非常特别GR＆ouml;道路它是99％，和/ 或者从第二子区域（20）衍生的，其是至少部分包围所述第一子区域（19）和/或在其中Abregungs-或Schaltlichtb BUNCH（6）具有一个零点，瓦特BEAR而探测器在AU的同时大街＆ 源自第一和第二子区域（19,20）外部的荧光至少部分地被消隐并且未被检测到

公开(公告)号：WO2017191121A1

公开(公告)日：2017-11-09

申请号：PCT/EP2017/060390

申请日：2017-05-02

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： FAHRBACH, Florian ,  SIECKMANN, Frank ,  SCHUMANN, Christian ,  SCHLICKER, Oliver

IPC: G02B21/00 ,  G02B21/16 ,  G02B21/36 ,  G02B27/58

CPC classification number: G02B21/0076 ,  G02B21/0032 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58 ,  G02B2207/113 ,  G02B2207/129

Abstract: Die Erfindung betrifft ein Mikroskop, aufweisend eine Beleuchtungsoptik zur Fluoreszenzanregung von Punktlichtquellen einer Probe, eine Detektionsoptik und eine Kamera mit einem Sensor, wobei die Dichte der Punktlichtquellen zur Minimierung der Überdeckung hintereinander oder nahe nebeneinander liegender Punktlichtquellen in jedem von der Kamera aufgenommenen Bild gering gehalten, und wobei im Strahlengang der Detektionsoptik ein Mittel zur Unterteilung der Detektionsapertur in einzelne Teilaperturen vorgesehen ist, so dass die Bilder, die von den einzelnen Teilaperturen auf dem Kamerasensor erzeugt werden, ein Objektvolumen aus unterschiedlichen Raumrichtungen abbilden.

Abstract translation:

本发明涉及一种显微镜，其包括用于样品，检测光学系统和一个传感器的相机，点光源的荧光激发的照明光学部件，其特征在于的所述点光源以减少导航用途的密度处于或接近相邻的点状光源berdeckung一个在另一个后面 在每个由相机图像捕获保持在较低水平，并且其中所述检测的光路中光学器件，用于将所述检测孔的装置的个别子孔径设置，使得通过在所述相机传感器的各个子孔径产生的图像，映射来自在空间不同方向上的对象体

公开(公告)号：WO2017144619A1

公开(公告)日：2017-08-31

申请号：PCT/EP2017/054244

申请日：2017-02-23

Applicant: SINGLE TECHNOLOGIES AB

Inventor： STUBBE, Raoul ,  SAHLGREN, Bengt ,  STRÖMQUIST, Johan

IPC: G01N21/64 ,  G01N21/47 ,  G02B21/00 ,  G02B21/33 ,  G02B21/26

CPC classification number: G01N21/6458 ,  G01N21/474 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/0833 ,  G01N2201/103 ,  G02B19/0019 ,  G02B19/0057 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/26 ,  G02B21/33 ,  G02B27/1006 ,  G02B27/141

Abstract: Apparatus for imaging a sample (109), said apparatus comprising: illumination means (101, 102, 103, 104, 105, 106, 107, 108) for illuminating said sample (109) simultaneously in a line focus or an array of foci; and detection means (108, 107, 106b, 111, 112, 113) for detecting photons emitted or scattered from a sample (109) simultaneously in an array of fields of view; wherein an array of sub-observation volumes in a sample (109), from which photons are emitted or scattered during imaging, is defined by the volumes in space where the line focus or array of foci from the illumination means (101, 102, 103, 104, 105, 106, 107, 108) overlap with the corresponding array of field of views of the detection means (108, 107, 106b, 111, 112, 113); a sample holder (110, 110a), preferably a cylindrical sample holder (110, 110a), configured to hold the sample (109) at a surface thereof, said sample holder (110, 110a) being rotatably arranged such that at least a portion of said sample (109) can be transported through at least one of said sub-observation volumes by rotating the sample holder (110, 110a).

Abstract translation: （109）成像的设备，所述设备包括：照明装置（101,102,103,104,105,106,107,108），用于同时照亮所述样品（109）和所述样品 线焦点或焦点阵列; 和检测装置（108,107,106b，111,112,113），用于同时在一个视场阵列中检测从样本（109）发射或散射的光子; 其中在成像过程中从其发射或散射光子的样本（109）中的子观察体积的阵列由空间体积限定，其中来自照明装置（101,102,103）的线焦点或焦点阵列 ，104,105,106,107,108）与所述检测装置（108,107,106b，111,112,113）的所述视场的对应阵列重叠。 样品保持器（110,110a），优选圆柱形样品保持器（110,110a），被配置为将样品（109）保持在其表面处，所述样品保持器（110,110a）可旋转地布置成使得至少一部分 可以通过旋转样本保持器（110,110a）将所述样本（109）的至少一个传送通过所述子观察容积中的至少一个。

公开(公告)号：WO2017075628A1

公开(公告)日：2017-05-04

申请号：PCT/US2016/059902

申请日：2016-11-01

Applicant: HOWARD HUGHES MEDICAL INSTITUTE

Inventor： SVOBODA, Karel ,  FLICKINGER, Daniel ,  SOFRONIEW, Nicholas

IPC: G02B21/18 ,  G02B21/36 ,  G02B27/00

CPC classification number: G02B21/006 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/33 ,  G02B21/367 ,  G02B26/101 ,  G06T11/60 ,  G06T2207/10061 ,  G06T2207/30016 ,  H04N5/2254 ,  H04N5/2256 ,  H04N5/23203 ,  H04N5/23238

Abstract: A microscope system including: a source of light; a sample objective configured for focusing the light at a focal plane within a sample; a remote focus unit configured for changing a position of the focal plane along an axis perpendicular to the focal plane; one or more optical element configured for directing the focused light to a location within the focal plane; and a detector configured for detecting light emitted from the focal plane within the sample; wherein the one or more optical element is located after the remote focus unit along a beam path of the light from the source to the sample objective, such that the changing the position of the focal plane along the axis is performed before the directing the focused light to the location within the focal plane.

Abstract translation: 显微镜系统包括：光源; 样品物镜，被配置用于将光聚焦在样品内的焦平面上; 远程聚焦单元，其被配置用于沿着垂直于所述焦平面的轴改变所述焦平面的位置; 一个或多个光学元件，所述一个或多个光学元件被配置成用于将聚焦的光引导至焦平面内的位置; 以及检测器，被配置用于检测样品内从焦平面发射的光; 其中所述一个或多个光学元件沿着从所述源到所述样品物镜的光的光路径位于所述远程聚焦单元之后，使得在引导所述聚焦光之前执行所述焦平面沿所述轴的位置改变 到焦平面内的位置。

公开(公告)号：WO2017067998A1

公开(公告)日：2017-04-27

申请号：PCT/EP2016/075116

申请日：2016-10-19

Applicant: DEUTSCHES KREBSFORSCHUNGSZENTRUM

Inventor： ENGELHARDT, Johann

IPC: G02B21/00 ,  G02B21/06 ,  G02B5/30 ,  G02B27/09 ,  G02B27/42

CPC classification number: G02B27/0927 ,  G02B5/3083 ,  G02B21/0076 ,  G02B21/06 ,  G02B27/42

Abstract: A segmented chromatic phase plate (1) comprises a main axis (8) and at least three stacks (2 to 5) of optical flats (6, 7). The at least three stacks (2 to 5) are arranged around the main axis (8). Each stack (2 to 5) comprises at least two plane-parallel optical flats (6, 7) made of different materials (11, 12). Each of the at least three stacks (2 to 5) is stacked along the main axis (8), and each of the at least three stacks (2 to 5) has a same overall height along the main axis (8). The materials (11, 12) and the thicknesses of the optical flats (6, 7) in the at least three stacks (2 to 5) are selected such that optical path lengths of light of a first wavelength passing through the different ones of the at least three stacks (2 to 5) along the main axis (8) differ by integer multiples of the first wavelength, whereas optical path lengths of light of a second wavelength passing through the different ones of the at least three stacks (2 to 5) along the main axis (8) differ by integer multiples plus defined fractions of the second wavelength. The two materials (11, 12) of the at least two optical flats (6, 7) of all of the at least three stacks (2 to 5) have different refractive indices (n0(λ), n1(λ)) for both the first and second wavelengths; and derivatives of the differences between the optical path lengths with respect to wavelength (λ) are zero at at least one of the first and second wavelengths.

Abstract translation: 分段彩色相位板（1）包括主轴线（8）和至少三个光学平面（6,7）的叠层（2至5）。 至少三个堆叠（2至5）围绕主轴线（8）布置。 每个堆叠（2至5）包括由不同材料（11,12）制成的至少两个平面平行的光学平面（6,7）。 至少三个堆叠（2至5）中的每一个沿着主轴线（8）堆叠，并且至少三个堆叠（2至5）中的每一个沿着主轴线（8）具有相同的总高度。 至少三个叠层（2至5）中的光学平面（6,7）的材料（11,12）和厚度被选择为使得第一波长的光的光路长度穿过不同的 沿着主轴线（8）的至少三个堆叠（2至5）相差整数倍的第一波长，而穿过至少三个堆叠（2至5）中的不同堆叠的第二波长的光的光路长度 ）沿着主轴线（8）相差整数倍加上第二波长的定义分数。 所有至少三个叠层（2至5）的至少两个光学平面（6,7）的两种材料（11,12）具有不同的折射率（n0（λ），n1（λ）） 第一和第二波长; 并且在第一和第二波长的至少一个中，光路长度相对于波长（λ）之间的差异的导数为零。

公开(公告)号：WO2017060385A1

公开(公告)日：2017-04-13

申请号：PCT/EP2016/073928

申请日：2016-10-06

Applicant: DEUTSCHES KREBSFORSCHUNGSZENTRUM

Inventor： ENGELHARDT, Johann

IPC: G02B21/00 ,  G02B27/28

CPC classification number: G02B21/0032 ,  G01N21/6458 ,  G02B21/0076 ,  G02B27/283

Abstract: A fluorescence microscope instrument (1 ) includes a light source (2) providing light (3) to be directed to a sample (4); a detector (5) detecting fluorescence light (5) emitted out of the sample (4); an objective (7) focusing the light (3) from the light source (2) into a focal area within the sample (4) and collecting the fluorescence light (6) emitted out of the focal area to be detected by the detector (5); and a beam path separator (9) arranged in a beam path of the light (3) from the light source (2) between the light source (2) and the objective (7) and in a beam path of the fluorescence light (6) between the objective (7) and the detector (5). Wavelengths of the light (3) to be directed to the sample (4) and of the light (6) to be detected by the detector fall into a range of wavelengths extending from a low end wavelength over at least 20 % of the low end wavelength. The beam path separator (9) separates the beam path of the fluorescence light (6) from the beam path of the light (3) from the light source (2) in that it is transferable between a first state in which it is transparent for light of any wavelength falling in the range of wavelengths and coming along the beam path of the light (3) from the light source (2), and a second state in which it is transparent for light of any wavelength falling in the range of wavelengths and coming along the beam path of the fluorescence light (6) from the sample (4).

Abstract translation: 荧光显微镜仪器（1）包括提供要被引导到样品（4）的光（3）的光源（2）; 检测器（5），检测从样品（4）发射的荧光（5）; 将来自光源（2）的光（3）聚焦到样品（4）内的聚焦区域并且收集由检测器（5）检测的聚焦区域外发射的荧光（6）的物镜（7） ）; 以及光束路径分离器（9），其布置在来自光源（2）和光源（2）之间的光（3）的光路中，并且在荧光（6）的光束路径中 ）在目标（7）和检测器（5）之间。 被引导到样品（4）的光（3）的波长和由检测器检测的光（6）的波长落入从低端波长延伸到低端的至少20％的波长范围 波长。 光束路径分离器（9）将荧光（6）的光束路径从光源（3）的光束路径与光源（2）分离，因为它可以在其透明的第一状态 落在波长范围内的任何波长的光和来自光源（2）的光（3）的光束路径的光，以及对于波长范围内的任何波长的光是透明的第二状态 并沿着来自样品（4）的荧光（6）的光束路径。

公开(公告)号：WO2017043000A1

公开(公告)日：2017-03-16

申请号：PCT/JP2016/003158

申请日：2016-07-01

Applicant: SONY CORPORATION

Inventor： NAGAE, Satoshi

IPC: A61B1/00 ,  A61B1/04 ,  A61B5/00 ,  G02B21/00 ,  G01N21/64 ,  G02B21/16 ,  G02B21/36 ,  G02B27/10

CPC classification number: A61B1/043 ,  A61B1/00006 ,  A61B1/0005 ,  A61B1/00186 ,  A61B1/00188 ,  A61B1/042 ,  A61B1/055 ,  A61B5/0071 ,  A61B2562/0242 ,  G01N21/6456 ,  G01N21/6458 ,  G01N2021/6463 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B21/365 ,  G02B27/1013

Abstract: Provided is a medical imaging device comprising: a color separation prism that has a dichroic film configured to split light into first light belonging to a visible light wavelength band and second light belonging to a fluorescence wavelength band; a fluorescence image sensor that is provided at an output side of the color separation prism and that is configured to image at least part of the second light belonging to the fluorescence wavelength band separated by the dichroic film; a visible light image sensor that is provided at the output side of the color separation prism and that is configured to image at least part of the first light belonging to the visible light wavelength band separated by the dichroic film; and a bandpass filter that is disposed between the color separation prism and the fluorescence image sensor, wherein the fluorescence image sensor and the visible light image sensor are arranged such that an optical path difference between an optical path length of a fluorescence optical path for the second light imaged on the fluorescence image sensor via the color separation prism and an optical path length of a visible light optical path for the first light imaged on the visible light image sensor via the color separation prism corresponds to an amount of a shift between a fluorescence imaging position and a visible light imaging position, the shift being generated by an imaging lens positioned at an input side of the color separation prism, and wherein the fluorescence imaging position is an imaging position of filtered second light, which results from passing the second light through the bandpass filter, such that the amount of shift is based on the filtered second light.

Abstract translation: 本发明提供一种医疗摄像装置，其特征在于，包括：分色棱镜，其具有将光分为属于可见光波长带的第一光和属于荧光波长带的第二光的二向色膜; 荧光图像传感器，其设置在所述分色棱镜的输出侧，并且被配置为对属于由所述二向色膜隔开的荧光波长带的所述第二光的至少一部分进行成像; 所述可见光图像传感器设置在所述分色棱镜的输出侧，并且被配置为对由所述二向色膜隔开的属于所述可见光波长带的所述第一光的至少一部分进行成像; 以及配置在所述分色棱镜和所述荧光图像传感器之间的带通滤光器，其中，所述荧光图像传感器和所述可见光图像传感器被配置为使得所述荧光光学路径的光路长度与所述第二 通过分色棱镜在荧光图像传感器上成像的光，并且经由分色棱镜成像在可见光图像传感器上的第一光的可见光光路的光路长度对应于荧光成像 位置和可见光成像位置，所述偏移由位于所述分色棱镜的输入侧的成像透镜产生，并且其中所述荧光成像位置是通过所述第二光通过的滤光后的第二光的成像位置 带通滤波器，使得移位量基于滤波的第二光。