公开(公告)号：WO2011158037A2

公开(公告)日：2011-12-22

申请号：PCT/GB2011/051133

申请日：2011-06-17

Applicant: LGC LIMITED ,  DEBENHAM, Paul Gerald ,  MOORE, David John

Inventor： DEBENHAM, Paul Gerald ,  MOORE, David John

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  G01N1/02 ,  G01N2001/007 ,  G01N2001/028

Abstract: A method for amplifying nucleic acid from a higher eukaryotic, such as mammalian or plant, nucleic acid source, the method comprising (a) contacting a sampling device with the source of higher eukaryotic, such as mammalian or plant, nucleic acid such that following said contacting, higher eukaryotic such as mammalian or plant nucleic acid-containing material is adhered to at least part of the sampling device, wherein the sampling device, or part thereof to which the nucleic acid-containing material is adhered, is made of a suitable polymeric material; (b) introducing the sampling device or part thereof to which the nucleic acid- containing material is adhered into a reaction vessel which contains a reaction mixture for carrying out a nucleic acid amplification reaction, without any prior treatment of the nucleic acid-containing material; and (c) performing a nucleic acid amplification reaction.

Abstract translation: 用于从高等真核生物（例如哺乳动物或植物）核酸源扩增核酸的方法，所述方法包括（a）使取样装置与较高真核生物来源如哺乳动物或植物核酸接触，使得遵循所述 接触较高的真核生物如哺乳动物或植物含核酸的材料粘附在采样装置的至少一部分上，其中所述采样装置或其含有核酸材料的部分由合适的聚合物 材料; （b）将含有含核酸材料的取样装置或其部分引入含有用于进行核酸扩增反应的反应混合物的反应容器中，而无需对含核酸材料进行任何处理; 和（c）进行核酸扩增反应。

公开(公告)号：WO2011158037A3

公开(公告)日：2012-03-29

申请号：PCT/GB2011051133

申请日：2011-06-17

Applicant: LGC LTD ,  DEBENHAM PAUL GERALD ,  MOORE DAVID JOHN

Inventor： DEBENHAM PAUL GERALD ,  MOORE DAVID JOHN

IPC: C12Q1/68 ,  A61B10/00 ,  G01N33/543

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  G01N1/02 ,  G01N2001/007 ,  G01N2001/028

Abstract: A method for amplifying nucleic acid from a higher eukaryotic, such as mammalian or plant, nucleic acid source, the method comprising (a) contacting a sampling device with the source of higher eukaryotic, such as mammalian or plant, nucleic acid such that following said contacting, higher eukaryotic such as mammalian or plant nucleic acid-containing material is adhered to at least part of the sampling device, wherein the sampling device, or part thereof to which the nucleic acid-containing material is adhered, is made of a suitable polymeric material; (b) introducing the sampling device or part thereof to which the nucleic acid- containing material is adhered into a reaction vessel which contains a reaction mixture for carrying out a nucleic acid amplification reaction, without any prior treatment of the nucleic acid-containing material; and (c) performing a nucleic acid amplification reaction.

Abstract translation: 用于从高等真核生物（例如哺乳动物或植物）核酸源扩增核酸的方法，所述方法包括（a）使取样装置与较高真核生物来源如哺乳动物或植物核酸接触，使得遵循所述 接触较高的真核生物如哺乳动物或植物含核酸的材料粘附在采样装置的至少一部分上，其中所述采样装置或其含有核酸材料的部分由合适的聚合物 材料; （b）将含有含核酸材料的取样装置或其部分引入含有用于进行核酸扩增反应的反应混合物的反应容器中，而无需对含核酸材料进行任何处理; 和（c）进行核酸扩增反应。

公开(公告)号：WO2009053679A1

公开(公告)日：2009-04-30

申请号：PCT/GB2008/003555

申请日：2008-10-21

Applicant: LGC LIMITED ,  UNIVERSITY OF SOUTHAMPTON ,  GALE, Nittaya ,  DEBENHAM, Paul ,  FRENCH, David, John ,  HOWARD, Rebecca ,  MCDOWELL, David, Gordon ,  BROWN, Tom

Inventor： GALE, Nittaya ,  DEBENHAM, Paul ,  FRENCH, David, John ,  HOWARD, Rebecca ,  MCDOWELL, David, Gordon ,  BROWN, Tom

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2527/107 ,  C12Q2525/186 ,  C12Q2525/151

Abstract: A method for determining the number of tandem repeats in a target polynucleotide, the method comprising (a) providing a sample containing the target polynucleotide, wherein one or more of the tandem repeats in the target polynucleotide is in single stranded form, (b) hybridising a labelled probe oligonucleotide to the single stranded portion of the target polynucleotide, wherein the probe oligonucleotide is complementary to at least one of the tandem repeats, and at least 5 nucleotides of the probe oligonucleotide are complementary to the tandem repeats, in the single stranded portion of the target polynucleotide, and (c) determining the number of tandem repeats in the target polynucleotide based on the hybridisation of the probe oligonucleotide to the single stranded portion of the target polynucleotide.

Abstract translation: 一种用于确定靶多核苷酸中的串联重复数目的方法，所述方法包括（a）提供含有靶多核苷酸的样品，其中靶多核苷酸中的一个或多个串联重复单位为单链形式，（b）杂交 对靶多核苷酸的单链部分的标记探针寡核苷酸，其中所述探针寡核苷酸与至少一个串联重复序列互补，并且探针寡核苷酸的至少5个核苷酸与串联重复序列互补，在单链部分 和（c）基于探针寡核苷酸与靶多核苷酸的单链部分的杂交来确定靶多核苷酸中串联重复序列的数目。