公开(公告)号：WO1998016624A1

公开(公告)日：1998-04-23

申请号：PCT/DE1997002277

申请日：1997-10-04

Applicant: BUNA SOW LEUNA OLEFINVERBUND GMBH ,  LEHMANN, Olaf ,  BERNDT, Christiane ,  MENZEL, Klaus-Dieter ,  DRIESCH, Dominik ,  WULFES, Christian ,  GLIEM, Toralf ,  CHRISTNER, Arnulf ,  HILLIGER, Matthias ,  MIERSCH, Karl

Inventor： BUNA SOW LEUNA OLEFINVERBUND GMBH

IPC: C12N01/00

CPC classification number: C12N1/32

Abstract: Methylotrophic micro-organisms are cultivated in known manner on the carbon substrate, methanol, using continuous measuring and secondary feeding processes in order to restrict the methanol concentration to very low values. According to the invention, a cultivation method comprising a plurality of cultivation stages is carried out in agitated and aerated bioreactors, the average methanol concentration decreasing from one stage to the next owing to suitable feeding methods. To that end, the methanol fed in during the cultivation stage (k-2) is proportional to the alkaline solution added in metered amounts during the regulation of the pH, in the cultivation stage (k-1), as the specific dosage, and in the final cultivation stage (k) first as the specific and then as the pO2-regulated dosage. This method prevents an accumulation of toxic intermediates and avoids the need to measure and regulate the methanol concentration during the entire cultivation period, such that costs are considerably reduced, in particular when the method is applied on an industrial scale.

Abstract translation: 在使用连续测量和Nachfütterungsverfahren以已知的方式在碳基材甲醇的甲基的微生物的培养来限制到非常低的值的甲醇浓度。 根据本发明，在描述和搅拌的生物反应器充气其中由合适Zufütterungsmethoden甲醇浓度从阶段平均降低至阶段的k阶段培养过程。 该Methanolzufütterung在培育阶段（K-2）成比例，在培育阶段中pH控制液中的剂量（K-1）作为施加的剂量和在最终的培养步骤（k）作为第一压花和进一步氧分压 控制用量。 该方法防止了有毒的中间体的累积。 整个培养过程中不需要的测量和控制甲醇的浓度。 这使得该方法在一个显著成本降低有助于特别是在工业规模上。