公开(公告)号：WO1995016911A1

公开(公告)日：1995-06-22

申请号：PCT/US1994013852

申请日：1994-12-06

Applicant: PERKIN-ELMER CORPORATION ,  MADABHUSHI, Ramakrishna, S. ,  MENCHEN, Steven, M. ,  EFCAVITCH, J., William ,  GROSSMAN, Paul, D.

Inventor： PERKIN-ELMER CORPORATION

IPC: G01N27/447

CPC classification number: G01N27/44752 ,  G01N27/44747

Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20 DEG C to about 50 DEG C, (ii) concentration in a separation medium in the range between about 0.001 % to about 10 % (weight/volume), (iii) molecular weight in the range of about 5 x 10 to about 1 x 10 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

Abstract translation: 本发明提供了不带电的水溶性二氧化硅吸附聚合物，用于抑制毛细管电泳中的电渗流和降低毛细管电泳中的分析物 - 壁相互作用。 在本发明的一个方面，一种或多种这样的聚合物用作用于通过毛细管电泳分离生物分子例如多核苷酸，多糖，蛋白质等的分离介质的组分。 通常，这种聚合物的特征在于（i）在约20℃至约50℃之间的温度范围内的水溶性，（ii）分离介质中的浓度在约0.001％至约10％（重量/体积） ），（iii）约5×10 3至约1×10 6道尔顿范围内的分子量，和（iv）在约6至约6范围内pH范围的水性介质中不存在带电基团 在一个实施方案中，本发明的聚合物选自聚内酰胺类，如聚乙烯吡咯烷酮; N，N-二取代的聚丙烯酰胺; 和N-取代的聚丙烯酰胺。 根据本发明的方法，足够量的聚合物吸附到毛细管表面以建立一个高粘度的区域，从而将分析物与壁隔离并阻止双电层在电场下移动。

公开(公告)号：WO2011102998A3

公开(公告)日：2011-08-25

申请号：PCT/US2011/024132

申请日：2011-02-09

Applicant: SEQUENOM, INC. ,  LAPIDUS, Stanley, N. ,  THOMPSON, John, F. ,  LIPSON, Doron ,  MILOS, Patrice, M. ,  EFCAVITCH, J., William ,  LETOVSKY, Stanley

Inventor： LAPIDUS, Stanley, N. ,  THOMPSON, John, F. ,  LIPSON, Doron ,  MILOS, Patrice, M. ,  EFCAVITCH, J., William ,  LETOVSKY, Stanley

IPC: C12Q1/68

Abstract: The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus.

公开(公告)号：WO1995016910A1

公开(公告)日：1995-06-22

申请号：PCT/US1994012739

申请日：1994-11-04

Applicant: PERKIN-ELMER CORPORATION ,  MADABHUSHI, Ramakrishna, S. ,  MENCHEN, Steven, M. ,  EFCAVITCH, J., William ,  GROSSMAN, Paul, D.

Inventor： PERKIN-ELMER CORPORATION

IPC: G01N27/447

CPC classification number: G01N27/44752 ,  G01N27/44747

Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20 DEG C to about 50 DEG C, (ii) concentration in a separation medium in the range between about 0.001 % to about 10 % (weight/volume), (iii) molecular weight in the range of about 5 x 10 to about 1 x 10 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

Abstract translation: 本发明提供了不带电的水溶性二氧化硅吸附聚合物，用于抑制毛细管电泳中的电渗流和降低毛细管电泳中的分析物 - 壁相互作用。 在本发明的一个方面，一种或多种这样的聚合物用作用于通过毛细管电泳分离生物分子例如多核苷酸，多糖，蛋白质等的分离介质的组分。 通常，这种聚合物的特征在于（i）在约20℃至约50℃之间的温度范围内的水溶性，（ii）分离介质中的浓度在约0.001％至约10％（重量/体积） ），（iii）约5×10 3至约1×10 6道尔顿范围内的分子量，和（iv）在约6至约6范围内pH范围的水性介质中不存在带电基团 在一个实施方案中，本发明的聚合物选自聚内酰胺类，如聚乙烯吡咯烷酮; N，N-二取代的聚丙烯酰胺; 和N-取代的聚丙烯酰胺。 根据本发明的方法，足够量的聚合物吸附到毛细管表面以建立一个高粘度的区域，从而将分析物与壁隔离并阻止双电层在电场下移动。

公开(公告)号：WO2009124254A1

公开(公告)日：2009-10-08

申请号：PCT/US2009/039475

申请日：2009-04-03

Applicant: HELICOS BIOSCIENCES CORPORATION ,  EFCAVITCH, J., William ,  SIDDIQI, Suhaib ,  BUZBY, Philip ,  MITCHELL, Judith ,  KRZYMANSKA-OLEJNIK, Edyta ,  MARAPPAN, Subramanian ,  BAI, Xiaopeng ,  ROY, Atanu ,  JAROSZ, Mirna ,  BOWERS, Jayson

Inventor： EFCAVITCH, J., William ,  SIDDIQI, Suhaib ,  BUZBY, Philip ,  MITCHELL, Judith ,  KRZYMANSKA-OLEJNIK, Edyta ,  MARAPPAN, Subramanian ,  BAI, Xiaopeng ,  ROY, Atanu ,  JAROSZ, Mirna ,  BOWERS, Jayson

IPC: C12Q1/68 ,  C07K7/00 ,  C07H19/073 ,  C07H19/173

CPC classification number: C07H19/073 ,  C07H19/173

Abstract: The invention provides for novel nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.

Abstract translation: 本发明提供新的核苷酸类似物及其使用方法，例如用于测序核酸。

公开(公告)号：WO1995021378A1

公开(公告)日：1995-08-10

申请号：PCT/US1995001487

申请日：1995-02-06

Applicant: PERKIN-ELMER CORPORATION ,  HING, Paul, A. ,  HOFF, Louis, B. ,  LACHENMEIER, Eric, W. ,  MEAD, Dennis, E. ,  SIMKINS, Barry, A. ,  EFCAVITCH, J., William

Inventor： PERKIN-ELMER CORPORATION

IPC: G01N27/447

CPC classification number: G01N27/44721

Abstract: The present invention relates to a fluorescent polynucleotide analyzer system which utilizes electrophoresis, and in particular, capillary-based electrophoresis. The analyzer system of the present invention is comprised of, in combination, a capillary containing an electrophoretic separation medium, a means for introducing a mixture of fluorescently labeled polynucleotide fragments into the capillary, detection means for detecting the fluorescently labeled polynucleotide fragments, including the simultaneous detection of multiple fluorescent labels, and, means for introducing and removing electrophoretic separation medium from the capillary.

Abstract translation: 本发明涉及利用电泳，特别是毛细管电泳的荧光多核苷酸分析仪系统。 本发明的分析器系统组合包括含有电泳分离介质的毛细管，用于将荧光标记的多核苷酸片段的混合物引入毛细管的装置，用于检测荧光标记的多核苷酸片段的检测装置，包括同时 检测多个荧光标记物，以及用于从毛细管引入和去除电泳分离介质的装置。

公开(公告)号：WO2016028802A8

公开(公告)日：2017-04-06

申请号：PCT/US2015045729

申请日：2015-08-18

Applicant: MOLECULAR ASSEMBLIES INC ,  EFCAVITCH J WILLIAM

Inventor： EFCAVITCH J WILLIAM

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12P19/34 ,  Y02P20/582

Abstract: The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using renewable initiators coupled to a solid support. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template.

Abstract translation: 本发明提供了使用与固体支持物偶合的可再生引发剂合成多核苷酸（例如DNA和RNA）的改进方法。 使用本发明的方法，多核苷酸的特异性序列可以在碱性水溶液环境中从头合成，而不使用核酸模板。

公开(公告)号：WO2016028802A1

公开(公告)日：2016-02-25

申请号：PCT/US2015/045729

申请日：2015-08-18

Applicant: MOLECULAR ASSEMBLIES, INC. ,  SIDDIQI, Suhaib ,  EFCAVITCH, J., William

Inventor： SIDDIQI, Suhaib

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12P19/34 ,  Y02P20/582

Abstract: The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using renewable initiators coupled to a solid support. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo , base by base, in an aqueous environment, without the use of a nucleic acid template.

Abstract translation: 本发明提供了使用与固体支持物偶联的可再生引发剂合成多核苷酸（例如DNA和RNA）的改进方法。 使用本发明的方法，多核苷酸的特异性序列可以在碱性水溶液环境中从头合成，而不使用核酸模板。

公开(公告)号：WO2011102998A2

公开(公告)日：2011-08-25

申请号：PCT/US2011024132

申请日：2011-02-09

Applicant: HELICOS BIOSCIENCES CORP ,  LAPIDUS STANLEY N ,  THOMPSON JOHN F ,  LIPSON DORON ,  MILOS PATRICE M ,  EFCAVITCH J WILLIAM ,  LETOVSKY STANLEY

Inventor： LAPIDUS STANLEY N ,  THOMPSON JOHN F ,  LIPSON DORON ,  MILOS PATRICE M ,  EFCAVITCH J WILLIAM ,  LETOVSKY STANLEY

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6809 ,  C12Q1/6883 ,  C12Q2600/156 ,  G06F19/18 ,  C12Q2535/122 ,  C12Q2537/16 ,  C12Q2537/165 ,  C12Q2563/159

Abstract: The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus.

Abstract translation: 本发明一般涉及用于检测胎儿核酸的方法和用于诊断胎儿异常的方法。 在某些实施方案中，本发明提供了用于确定胎儿核酸是否存在于母体样品中的方法，包括获得怀疑包括胎儿核酸的母体样品，并对样品进行测序反应以确定至少部分 Y染色体，从而确定胎儿核酸存在于样品中。 在其它实施方案中，本发明提供用于定量或定性分析以检测母体样品中的胎儿核酸的方法，而不管检测Y染色体的能力，特别是对于包括来自雌性胎儿的正常核酸的样品的检测能力。