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公开(公告)号：WO9904007A8
公开(公告)日：1999-04-22
申请号：PCT/US9814786
申请日：1998-07-15
Applicant: GENENCOR INT , GENENCOR INT BV , QUAX WILHELMUS J
Inventor： QUAX WILHELMUS J
IPC: C12N15/09 , C07K14/32 , C12N1/21 , C12N15/31 , C12N15/62 , C12N15/75 , C12P21/02 , C12P21/04 , C12R1/07
CPC classification number: C12N15/75 , C07K14/32 , C07K2319/00
Abstract: The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis secretion factors SecDF. The present invention also provides improved methods for the secretion of heterologous or homologous proteins in gram-positive microorganisms.
Abstract translation: 本发明涉及革兰氏阳性微生物中的分泌。 本发明提供了枯草芽孢杆菌分泌因子SecDF的核酸和氨基酸序列。 本发明还提供了在革兰氏阳性微生物中分泌异源或同源蛋白质的改进方法。
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公开(公告)号：WO9904007A9
公开(公告)日：1999-05-20
申请号：PCT/US9814786
申请日：1998-07-15
Applicant: GENENCOR INT , GENENCOR INT BV , QUAX WILHELMUS J
Inventor： QUAX WILHELMUS J
IPC: C12N15/09 , C07K14/32 , C12N1/21 , C12N15/31 , C12N15/62 , C12N15/75 , C12P21/02 , C12P21/04 , C12R1/07
CPC classification number: C12N15/75 , C07K14/32 , C07K2319/00
Abstract: The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis secretion factors SecDF. The present invention also provides improved methods for the secretion of heterologous or homologous proteins in gram-positive microorganisms.
Abstract translation: 本发明涉及革兰氏阳性微生物中的分泌。 本发明提供枯草芽孢杆菌分泌因子SecDF的核酸和氨基酸序列。 本发明还提供了用于在革兰氏阳性微生物中分泌异源或同源蛋白质的改进方法。
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公开(公告)号：WO9904016A3
公开(公告)日：1999-04-15
申请号：PCT/US9814529
申请日：1998-07-14
Applicant: GENENCOR INT , GENENCOR INT BV , ESTELL DAVID A
Inventor： ESTELL DAVID A
Abstract: The present invention relates to the identification of novel cysteine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis cysteine proteases CP1, CP2 and CP3. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding CP1, CP2 or CP3. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a cysteine protease of the present invention.
Abstract translation: 本发明涉及革兰氏阳性微生物中新型半胱氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌半胱氨酸蛋白酶CP1，CP2和CP3的核酸和氨基酸序列。 本发明还提供具有编码CP1，CP2或CP3的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的半胱氨酸蛋白酶的清洁组合物。
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公开(公告)号：WO9914341A3
公开(公告)日：1999-05-06
申请号：PCT/US9818677
申请日：1998-09-08
Applicant: GENENCOR INT , GENENCOR INT BV , ESTELL DAVID A
Inventor： ESTELL DAVID A
IPC: C07H21/04 , C07K14/52 , C07K14/57 , C11D3/386 , C12N9/54 , C12N9/56 , C12N15/57 , C12N15/74 , C12N15/75 , C12P21/06 , C12N1/20 , C12N1/21 , C12Q1/68
Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.
Abstract translation: 本发明涉及革兰氏阳性微生物中新型金属蛋白酶（MP）的鉴定。 本发明提供了芽孢杆菌MP的核酸和氨基酸序列。 本发明还提供了具有部分或全部编码MP的基因的突变或缺失的宿主细胞。 本发明还提供了还包含编码所需异源蛋白质如酶的核酸的宿主细胞。 本发明还提供了包含本发明的MP的清洁组合物。
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公开(公告)号：WO9903984A3
公开(公告)日：1999-04-08
申请号：PCT/US9814647
申请日：1998-07-14
Applicant: GENENCOR INT , GENENCOR INT BV , ESTELL DAVID A
Inventor： ESTELL DAVID A
IPC: C12N15/09 , C11D3/386 , C12N1/21 , C12N9/18 , C12N9/48 , C12N9/56 , C12N15/00 , C12N15/75 , C12R1/07 , C12N15/57
CPC classification number: C12N9/54 , C11D3/38663 , C12N9/18 , C12N9/48 , C12N15/75
Abstract: The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
Abstract translation: 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。
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Patent Agency Ranking6.发明申请METHOD OF SEPARATING COMPONENTS IN A SAMPLE USING SILANE-TREATED SILICA FILTER MEDIA 审中-公开使用硅烷处理的二氧化硅过滤介质在样品中分离组分的方法
公开(公告)号：WO2005115580A3
公开(公告)日：2009-04-16
申请号：PCT/US2005014069
申请日：2005-04-21
Applicant: GENENCOR INT , DOW CORNING , GIBSON GARY L , HAYES KEITH , HENG MENG H , KOLLAR CSILLA , LANE THOMAS H , REVIS ANTHONY , STEELE LANDON M
Inventor： GIBSON GARY L , HAYES KEITH , HENG MENG H , KOLLAR CSILLA , LANE THOMAS H , REVIS ANTHONY , STEELE LANDON M
CPC classification number: B01J20/3259 , B01D15/361 , B01D15/3804 , B01J20/103 , B01J20/14 , B01J20/286 , B01J20/3204 , B01J20/3242 , B01J20/3244 , B01J20/3257 , B01J2220/4887 , Y10T436/25375
Abstract: The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have been reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash, oat hull ash, or diatomaceous earth.
Abstract translation: 本发明提供了从含有微粒和可溶性材料的样品中分离出一种或多种感兴趣组分的方法。 该方法包括以下步骤：（a）通过其表面硅烷醇基团与一种或多种硅烷反应的二氧化硅过滤介质过滤样品，和（b）同时捕获微粒并将可溶组分结合到二氧化硅过滤介质上。 随后从二氧化硅过滤介质中洗脱所结合的可溶性成分。 在本发明的一个实施方案中，不希望的可溶物质被经处理的二氧化硅过滤介质捕获，并且所需的目的组分从流通中回收。 在本发明的另一个实施方案中，感兴趣的不同成分从洗脱液和流通液中回收。 优选的经处理的二氧化硅过滤介质是硅烷处理的稻壳灰，燕麦壳灰或硅藻土。
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公开(公告)号：WO2008013747A3
公开(公告)日：2008-03-27
申请号：PCT/US2007016461
申请日：2007-07-20
Applicant: GENENCOR INT , KUMAR MANOJ
Inventor： KUMAR MANOJ
IPC: C11D3/386
CPC classification number: C11D3/386 , A01N63/02 , A01N65/00 , A01N2300/00 , A01N63/04
Abstract: Described herein is a composition for removing biofilm from surfaces. The composition is an enzyme mixture having at least three enzymes and resulting in the removal of at least 40% biofilm from the surface.
Abstract translation: 本文描述了用于从表面去除生物膜的组合物。 该组合物是具有至少三种酶并且导致从表面除去至少40％生物膜的酶混合物。
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公开(公告)号：WO2007001420A3
公开(公告)日：2007-05-18
申请号：PCT/US2005037641
申请日：2005-10-18
Applicant: GENENCOR INT , CHEN YIYOU , CHEN DAN , DAY ANTHONY G , ESTELL DAVID A , GENG WEI
Inventor： CHEN YIYOU , CHEN DAN , DAY ANTHONY G , ESTELL DAVID A , GENG WEI
IPC: C07K16/00
CPC classification number: C07K16/00 , C07K2317/92
Abstract: The invention is drawn to a method of isolating human antibodies.
Abstract translation: 本发明涉及分离人抗体的方法。
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公开(公告)号：WO2007044968A2
公开(公告)日：2007-04-19
申请号：PCT/US2006040394
申请日：2006-10-12
Applicant: GENENCOR INT , BECKER NATHANIEL T , CLARKSON KATHLEEN A , DALE DOUGLAS , FRYKSDALE BETH , GERBERT MARK S , PARTSUF MICHAEL , GRAVESEN TROELS
Inventor： BECKER NATHANIEL T , CLARKSON KATHLEEN A , DALE DOUGLAS , FRYKSDALE BETH , GERBERT MARK S , PARTSUF MICHAEL , GRAVESEN TROELS
CPC classification number: A61K9/14 , A23K20/189 , A23K40/30 , A23K50/75 , A61K9/16 , A61K9/5073 , A61K38/465 , A61K38/47 , A61K47/02 , C12N9/96 , C12N9/98
Abstract: ABSTRACT A stable, durable granule for feed compositions has a core, at least one active agent; and at least one coating. The active agent of the granule retains at least 50% activity, at least 60% activity, at least 70% activity, at least 80% activity after conditions selected from one or more of a) a feed pelleting process, b) a steam-heated feed pretreatment process, c) storage, d) storage as an ingredient in an unpelleted mixture, and e) storage as an ingredient in a feed base mix or a feed premix comprising at least one compound selected from trace minerals, organic acids, reducing sugars, vitamins, choline chloride, and compounds which result in an acidic or a basic feed base mix or feed premix.
Abstract translation: 摘要用于饲料组合物的稳定耐用颗粒具有核心，至少一种活性剂; 和至少一个涂层。 颗粒的活性剂在选自以下一种或多种条件之后保留至少50％的活性，至少60％的活性，至少70％的活性，至少80％的活性：a）饲料造粒工艺，b） 加热的饲料预处理过程，c）储存，d）作为成分储存在未搅拌的混合物中，和e）作为成分储存在饲料基料混合物或饲料预混物中，所述饲料预混物包含至少一种选自微量矿物质，有机酸， 糖类，维生素，氯化胆碱，以及导致酸性或碱性饲料基料混合物或饲料预混料的化合物。
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10.发明申请
公开(公告)号：WO2006073843A3
公开(公告)日：2007-04-05
申请号：PCT/US2005046474
申请日：2005-12-21
Applicant: GENENCOR INT , DUAN GANG , DUNN-COLEMAN NIGEL , LANTERO ORESTE J JR , PILGRIM CRAIG E , SHETTY JAYARAMA K
Inventor： DUAN GANG , DUNN-COLEMAN NIGEL , LANTERO ORESTE J JR , PILGRIM CRAIG E , SHETTY JAYARAMA K
IPC: C12P7/06
CPC classification number: C12N9/58 , A23L13/424 , A23L33/18 , C12N9/48 , C12P7/06 , C12P7/10 , C12P19/02 , Y02E50/16 , Y02E50/17
Abstract: The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.
Abstract translation: 本发明涉及生产乙醇的方法，并且在无烹饪发酵中减少残余淀粉的产生，包括在合适的发酵条件下，在低于淀粉糊化温度的温度下，使含有颗粒淀粉的底物与颗粒淀粉水解酶，蛋白酶和发酵微生物接触 淀粉底物的温度以产生乙醇，其中与基本相似的无蛋白酶进行的方法相比，乙醇生产增加，残留淀粉的量降低。
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