公开(公告)号：WO2011149701A2

公开(公告)日：2011-12-01

申请号：PCT/US2011/036722

申请日：2011-05-17

Applicant: AIR PRODUCTS AND CHEMICALS, INC. ,  GIBSON, Daniel, James ,  SCHMIDT, Marna, Beth ,  HIMES, Michael, Robert

Inventor： GIBSON, Daniel, James ,  SCHMIDT, Marna, Beth ,  HIMES, Michael, Robert

IPC: B26D7/10

CPC classification number: B26D7/10 ,  B26D3/18

Abstract: A method and apparatus for directing gas at cryogenic temperatures onto at least some of the contact surfaces of machinery used in a cutting process. The gas is supplied at a relatively high flow rate (preferably exceeding 1000 SCFH) and at cryogenic temperatures (e.g., below -70 degrees C). The temperature at which the coolant gas is supplied is preferably maintained within ±5 degrees F (±3 degrees C).

Abstract translation: 一种将低温气体引导到切割过程中使用的机械的至少一些接触表面的方法和装置。 气体以较高的流速（优选超过1000SCFH）和低温（例如低于-70℃）供应。 提供冷却剂气体的温度优选保持在±5华氏度（±3摄氏度）内。

公开(公告)号：WO2008137887A1

公开(公告)日：2008-11-13

申请号：PCT/US2008/062742

申请日：2008-05-06

Applicant: AIR PRODUCTS AND CHEMICALS, INC. ,  GHOSH, Ranajit ,  GIBSON, Daniel, James

Inventor： GHOSH, Ranajit ,  GIBSON, Daniel, James

IPC: B26D7/14

CPC classification number: B23Q11/1053 ,  Y10T82/10

Abstract: A machining method and an article manufactured therefrom, the method improving mechanical properties in a work surface by performing a very shallow machining pass using a cutting tool, in combination with application of a cryogenic fluid to the work surface and the cutting tool, the combination compressive force and cryogenic cooling increasing hardness, increasing compressive residual stress, and reducing surface roughness in the manufactured article.

Abstract translation: 一种机械加工方法及其制造方法，该方法通过使用切削工具进行非常浅的加工，结合在工作表面和切削工具上施加低温流体来提高工件表面的机械性能，组合压缩 强制和低温冷却增加硬度，增加压缩残余应力，并降低制品中的表面粗糙度。

公开(公告)号：WO2006047316A2

公开(公告)日：2006-05-04

申请号：PCT/US2005/037956

申请日：2005-10-21

Applicant: THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF THE NAVY ,  SANGHERA, Jasbinder, S. ,  PUREZA, Pablo, C. ,  KUNG, Fred, H. ,  GIBSON, Daniel ,  SHAW, Leslie, B. ,  AGGARWAL, Ishwar, D.

Inventor： SANGHERA, Jasbinder, S. ,  PUREZA, Pablo, C. ,  KUNG, Fred, H. ,  GIBSON, Daniel ,  SHAW, Leslie, B. ,  AGGARWAL, Ishwar, D.

IPC: G02B6/00

CPC classification number: G02B6/02328 ,  C03B37/0122 ,  C03B37/01274 ,  C03B37/025 ,  C03B37/02781 ,  C03B2201/60 ,  C03B2201/62 ,  C03B2201/70 ,  C03B2201/78 ,  C03B2201/80 ,  C03B2201/84 ,  C03B2201/86 ,  C03B2201/88 ,  C03B2203/12 ,  C03B2203/14 ,  C03B2203/16 ,  C03B2203/42 ,  C03B2205/10 ,  C03C11/00 ,  C03C13/043 ,  G02B6/02347 ,  G02B6/02361 ,  G02B6/02371

Abstract: A photonic band gap fiber and method of making thereof is provided. The fiber is made of a non-silica-based glass and has a longitudinal central opening, a microstructured region having a plurality of longitudinal surrounding openings, and a jacket. The air fill fraction of the microstructured region is at least about 40%. The fiber may be made by drawing a preform into a fiber, while applying gas pressure to the microstructured region. The air fill fraction of the microstructured region is changed during the drawing.

Abstract translation: 提供一种光子带隙光纤及其制造方法。 纤维由非二氧化硅基玻璃制成并具有纵向中央开口，具有多个纵向环绕开口的微结构化区域以及护套。 微结构化区域的空气填充分数至少约为40％。 纤维可以通过将预成型件拉成纤维来制造，同时向微结构化区域施加气压。 在绘图过程中，微结构区域的空气填充部分会发生变化。

公开(公告)号：WO2010129552A3

公开(公告)日：2011-03-24

申请号：PCT/US2010033551

申请日：2010-05-04

Applicant: UNIV FLORIDA ,  GIBSON DANIEL J ,  SCHULTZ GREGORY S ,  TULI SONAL S

Inventor： GIBSON DANIEL J ,  SCHULTZ GREGORY S ,  TULI SONAL S

IPC: A61N1/30 ,  A61N1/05

CPC classification number: A61N1/303 ,  A61N1/0424 ,  A61N1/044 ,  A61N1/0448

Abstract: Electromotive delivery of macromolecules can be provided using a delivery device including a first chamber for contacting a macromolecule delivery medium to a surface of a tissue or other anatomy of a patient; and a second chamber for contacting a receiving medium to the patient. A first electrode can be disposed in the first chamber so as to not directly contact the macromolecule delivery medium. A second medium having a buffering agent can be used to keep the first electrode from coming into direct contact with the macromolecule delivery medium. A second electrode can be disposed within the receiving medium in the second chamber such that the second electrode does not directly contact the patient. An electric field can be generated using the first and second electrodes in order to cause the macromolecule delivery medium to move into the tissue of interest from the first chamber of the delivery device.

Abstract translation: 可以使用包括用于使大分子递送介质与组织的表面或患者的其他解剖结构接触的第一室的输送装置来提供大分子的电动势输送; 以及用于使接收介质接触患者的第二腔室。 第一电极可以设置在第一室中，以便不直接接触大分子输送介质。 可以使用具有缓冲剂的第二介质来保持第一电极不与大分子输送介质直接接触。 第二电极可以设置在第二室中的接收介质内，使得第二电极不直接接触患者。 可以使用第一和第二电极产生电场，以便使大分子递送介质从输送装置的第一室移动到感兴趣的组织中。

公开(公告)号：WO2010151878A2

公开(公告)日：2010-12-29

申请号：PCT/US2010/040215

申请日：2010-06-28

Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. ,  SCHULTZ, Gregory, S. ,  GIBSON, Daniel, J.

Inventor： SCHULTZ, Gregory, S. ,  GIBSON, Daniel, J.

IPC: C12Q1/37 ,  C12N9/66 ,  G01N33/52

CPC classification number: C12Q1/37 ,  G01N33/542 ,  G01N2333/966

Abstract: The subject invention provides novel devices and methods for the detection and quantification of neutrophil elastase activity in biological samples.

Abstract translation: 本发明提供了用于检测和定量生物样品中嗜中性粒细胞弹性蛋白酶活性的新颖装置和方法。

公开(公告)号：WO2010141719A2

公开(公告)日：2010-12-09

申请号：PCT/US2010/037257

申请日：2010-06-03

Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION INC. ,  SCHULTZ, Gregory, S. ,  GIBSON, Daniel, J.

Inventor： SCHULTZ, Gregory, S. ,  GIBSON, Daniel, J.

IPC: G01N33/84 ,  G01N33/52

CPC classification number: G01N33/582 ,  G01N33/84 ,  G01N2800/20 ,  Y10T436/177692

Abstract: The subject invention presides novel devices and methods for the measurement of nitric oxide in biological samples, including wound fluid samples. These advantageous devices and methods can be used for clinicians to monitor the wound's nitric oxide metabolism and/or response to treatment.

Abstract translation: 本发明主张用于测量生物样品中一氧化氮的新颖的装置和方法，包括伤口液样品。 这些有利的装置和方法可用于临床医生监测伤口的一氧化氮代谢和/或对治疗的反应。

公开(公告)号：WO2009103027A2

公开(公告)日：2009-08-20

申请号：PCT/US2009/034153

申请日：2009-02-13

Applicant: SYNTHETIC GENOMICS, INC. ,  VENTER, Craig, J. ,  GIBSON, Daniel, G. ,  SMITH, Hamilton, O. ,  HUTCHISON, Clyde, A. ,  YOUNG, Lei

Inventor： VENTER, Craig, J. ,  GIBSON, Daniel, G. ,  SMITH, Hamilton, O. ,  HUTCHISON, Clyde, A. ,  YOUNG, Lei

IPC: C40B40/08

CPC classification number: C12P19/34 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/64 ,  C12N15/66

Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single- stranded (ss) DNA molecules of interest in vitro , wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g. , to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

Abstract translation: 本发明涉及在体外连接两个或多个目的双链（ds）或单链（ss）DNA分子的方法，其中第一DNA分子的远端区域和第二DNA分子的近端区域 每对共享序列同一性区域。 该方法允许以预定顺序和取向连接大量DNA片段，而不使用限制酶。 其可以用于例如合成产生的感兴趣的基因或基因组的亚片段。 还公开了用于执行该方法的套件。 可以使用连接DNA分子的方法来产生组合文库，其可用于产生例如通过密码子优化，基因优化和途径优化的最佳蛋白质表达。

公开(公告)号：WO2009048885A3

公开(公告)日：2009-04-16

申请号：PCT/US2008/079109

申请日：2008-10-07

Applicant: SYNTHETIC GENOMICS, INC. ,  GIBSON, Daniel, G. ,  BENDERS, Gwynedd, A. ,  YOUNG, Lei ,  GLASS, John, I. ,  VENTER, J., Craig ,  HUTCHISON, Clyde, A. ,  SMITH, Hamilton, O.

Inventor： GIBSON, Daniel, G. ,  BENDERS, Gwynedd, A. ,  YOUNG, Lei ,  GLASS, John, I. ,  VENTER, J., Craig ,  HUTCHISON, Clyde, A. ,  SMITH, Hamilton, O.

IPC: C12Q1/68

Abstract: A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.

公开(公告)号：WO2007032837A3

公开(公告)日：2007-05-24

申请号：PCT/US2006031214

申请日：2006-08-11

Applicant: J CRAIG VENTER INST ,  GIBSON DANIEL GLENN ,  SMITH HAMILTON O

Inventor： GIBSON DANIEL GLENN ,  SMITH HAMILTON O

IPC: C12N15/09 ,  C12N15/00

CPC classification number: C12N9/1252 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/64 ,  C12N15/66 ,  C12P19/34

Abstract: The present invention relates, e.g., to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising (a) chewing back the DNA molecules with an enzyme having an exonuclease activity, to yield single-stranded overhanging portions of each DNA molecule which contain a sufficient length of the region of sequence identity to hybridize specifically to each other; (b) specifically annealing the single-stranded overhangs; and (c) repairing single-stranded gaps in the annealed DNA molecules and sealing the nicks thus formed (ligating the nicked DNA molecules). The region of sequence identity generally comprises at least 20 non-palindromic nucleotides (nt), e.g., at least about 40 non-palindromic nt. In some embodiments of the invention, about 5% PEG is present during all steps of the reaction, and/or the repair reaction is achieved with Taq DNA polymerase and a compatible ligase, such as Taq DNA ligase. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.

Abstract translation: 本发明涉及例如使用分离的蛋白试剂用于连接两个目的双链（ds）DNA分子的体外方法，其中第一个DNA分子的远端区域和第二个DNA分子的近端区域 共享序列同一性区域，包括（a）用具有核酸外切酶活性的酶咀嚼DNA分子，以产生每个DNA分子的单链悬垂部分，其含有足够长度的序列同一性区域以特异性杂交至 彼此; （b）特异性退火单链突出端; （c）修复退火的DNA分子中的单链间隙并密封由此形成的切口（连接切口的DNA分子）。 序列同一性区域通常包含至少20个非回文核苷酸（nt），例如至少约40个非回文核苷酸。 在本发明的一些实施方案中，在所有反应步骤中存在约5％PEG，和/或用Taq DNA聚合酶和相容连接酶如Taq DNA连接酶实现修复反应。 该方法允许以预定的顺序和方向连接许多DNA片段，而不使用限制性内切酶。 它可以用于例如连接感兴趣的基因或基因组的合成产生的亚片段。

公开(公告)号：WO2006050362A3

公开(公告)日：2006-05-11

申请号：PCT/US2005/039484

申请日：2005-11-01

Applicant: PRECISIA, L.L.C. ,  LAWRENCE, Daniel P. ,  RIGNEY, Jennifer ,  GIBSON, Daniel

Inventor： LAWRENCE, Daniel P. ,  RIGNEY, Jennifer ,  GIBSON, Daniel

IPC: G06K19/077 ,  G08B13/24 ,  G09F3/03 ,  H05K1/18 ,  H05K3/30

Abstract: An electronic assembly and method for making the same includes a flexible substrate and a protective layer arranged adjacent to one side of the substrate. The protective layer has a first thickness and defines at least one hole. A first electrical component has a second thickness that is less than or equal to the first thickness. The first electrical component is received in the hole in the protective layer.