公开(公告)号：WO2011033375A3

公开(公告)日：2011-05-12

申请号：PCT/IB2010002337

申请日：2010-09-20

Applicant: SELEXIS SA ,  MERMOD NICOLAS ,  GIROD PIERRE-ALAIN ,  GRANDJEAN MELANIE ,  LE FOURN VALERIE ,  CALABRESE DAVID ,  REGAMEY ALEXANDRE

Inventor： MERMOD NICOLAS ,  GIROD PIERRE-ALAIN ,  GRANDJEAN MELANIE ,  LE FOURN VALERIE ,  CALABRESE DAVID ,  REGAMEY ALEXANDRE

IPC: C12N15/90 ,  C12N15/113

CPC classification number: C12N15/907 ,  C12N15/85 ,  C12N2830/46

Abstract: Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhanced a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric strucutre which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particualr a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.

Abstract translation: 公开了用于转基因表达的方法和真核宿主细胞。 可以对细胞进行治疗和/或修饰以增加同源重组（HR），减少非同源末端连接（NHEJ）和/或增强所述细胞中的HR / NHEJ比例。 可以用包含转基因的载体转染这样的细胞，其有利地整合到细胞的基因组中以形成可以包含多于200个转基因拷贝的连接结构。 有利地提供某些表达增强元件如MARs以进一步增强和/或促进转基因表达。 还公开了重组真核宿主细胞，特别是非灵长类动物宿主细胞，其包含编码蛋白质和/或RNA的转基因序列，特别是灵长类动物蛋白质和/或RNA，涉及穿过ER膜的易位和/ 或分泌跨细胞质膜。