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1.发明申请MATRIX ATTACHMENT REGIONS (MARS) FOR INCREASING TRANSCRIPTION AND USES THEREOF 审中-公开用于增加转录的矩阵附着区域（MARS）及其用途
公开(公告)号：WO2008023247A2
公开(公告)日：2008-02-28
申请号：PCT/IB2007/002404
申请日：2007-08-22
Applicant: SELEXIS S.A. , MERMOD, Nicolas , GIROD, Pierre Alain , CALABRESE, David , REGAMEY, Alexandre , DONINELLI-AROPE, Saline
Inventor： MERMOD, Nicolas , GIROD, Pierre Alain , CALABRESE, David , REGAMEY, Alexandre , DONINELLI-AROPE, Saline
CPC classification number: C12N15/63
Abstract: Isolated and purified MAR sequences of human and non-human animal origin are disclosed as are nucleotide sequences corresponding to or based on them. In particular, MARs and MAR constructs with high transcription and/or protein production enhancing activities are disclosed and so are methods for identifying such MARs, designing such MAR constructs and employing them, e.g., for high yield production of proteins.
Abstract translation: 公开了人和非人动物来源的分离和纯化的MAR序列以及对应于或基于它们的核苷酸序列。 具体而言，公开了具有高转录和/或蛋白质产生增强活性的MAR和MAR构建体，以及用于鉴定这种MAR的方法，设计这种MAR构建体并使用它们，例如用于高产量生产蛋白质的方法。
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2.发明申请
公开(公告)号：WO2011033375A2
公开(公告)日：2011-03-24
申请号：PCT/IB2010/002337
申请日：2010-09-20
Applicant: SELEXIS S.A. , MERMOD, Nicolas , GIROD, Pierre-Alain , GRANDJEAN, Mélanie , LE FOURN, Valérie , CALABRESE, David , REGAMEY, Alexandre
Inventor： MERMOD, Nicolas , GIROD, Pierre-Alain , GRANDJEAN, Mélanie , LE FOURN, Valérie , CALABRESE, David , REGAMEY, Alexandre
IPC: C12N15/90 , C12N15/113
CPC classification number: C12N15/907 , C12N15/85 , C12N2830/46
Abstract: Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhanced a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric strucutre which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particualr a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.
Abstract translation: 公开了用于转基因表达的方法和真核宿主细胞。 可以处理和/或修饰细胞以增加所述细胞中的同源重组（HR），减少非同源末端连接（NHEJ）和/或增强HR / NHEJ比率。 这些细胞可以用包含转基因的载体转染，其有利地整合到细胞的基因组中以形成可包含超过200个转基因拷贝的串联结构。 有利地提供某些表达增强元件如MARs以进一步增强和/或促进转基因表达。 还公开了重组真核宿主细胞，特别是非灵长类宿主细胞，其包含编码蛋白和/或RNA（特别是灵长类蛋白和/或RNA）的转基因序列，所述蛋白和/或RNA涉及穿过ER膜和/ 或通过细胞质膜分泌。
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3.发明申请
公开(公告)号：WO2011033375A3
公开(公告)日：2011-05-12
申请号：PCT/IB2010002337
申请日：2010-09-20
Applicant: SELEXIS SA , MERMOD NICOLAS , GIROD PIERRE-ALAIN , GRANDJEAN MELANIE , LE FOURN VALERIE , CALABRESE DAVID , REGAMEY ALEXANDRE
Inventor： MERMOD NICOLAS , GIROD PIERRE-ALAIN , GRANDJEAN MELANIE , LE FOURN VALERIE , CALABRESE DAVID , REGAMEY ALEXANDRE
IPC: C12N15/90 , C12N15/113
CPC classification number: C12N15/907 , C12N15/85 , C12N2830/46
Abstract: Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhanced a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric strucutre which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particualr a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.
Abstract translation: 公开了用于转基因表达的方法和真核宿主细胞。 可以对细胞进行治疗和/或修饰以增加同源重组（HR），减少非同源末端连接（NHEJ）和/或增强所述细胞中的HR / NHEJ比例。 可以用包含转基因的载体转染这样的细胞，其有利地整合到细胞的基因组中以形成可以包含多于200个转基因拷贝的连接结构。 有利地提供某些表达增强元件如MARs以进一步增强和/或促进转基因表达。 还公开了重组真核宿主细胞，特别是非灵长类动物宿主细胞，其包含编码蛋白质和/或RNA的转基因序列，特别是灵长类动物蛋白质和/或RNA，涉及穿过ER膜的易位和/ 或分泌跨细胞质膜。
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4.发明申请MATRIX ATTACHMENT REGIONS (MARS) FOR INCREASING TRANSCRIPTION AND USES THEREOF 审中-公开MATRIX附件区（MARS）用于增加转录及其用途
公开(公告)号：WO2008023247A3
公开(公告)日：2008-05-08
申请号：PCT/IB2007002404
申请日：2007-08-22
Applicant: SELEXIS SA , MERMOD NICOLAS , GIROD PIERRE ALAIN , CALABRESE DAVID , REGAMEY ALEXANDRE , DONINELLI-AROPE SALINE
Inventor： MERMOD NICOLAS , GIROD PIERRE ALAIN , CALABRESE DAVID , REGAMEY ALEXANDRE , DONINELLI-AROPE SALINE
CPC classification number: C12N15/63
Abstract: Isolated and purified MAR sequences of human and non-human animal origin are disclosed as are nucleotide sequences corresponding to or based on them. In particular, MARs and MAR constructs with high transcription and/or protein production enhancing activities are disclosed and so are methods for identifying such MARs, designing such MAR constructs and employing them, e.g., for high yield production of proteins.
Abstract translation: 人和非人动物来源的分离和纯化的MAR序列被公开为对应于或基于它们的核苷酸序列。 特别地，公开了具有高转录和/或蛋白质生产增强活性的MAR和MAR构建体，鉴定这样的MARs的方法也是如此，设计这样的MAR构建体并使用它们，例如高产量生产蛋白质。
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