公开(公告)号：WO2008023247A2

公开(公告)日：2008-02-28

申请号：PCT/IB2007/002404

申请日：2007-08-22

Applicant: SELEXIS S.A. ,  MERMOD, Nicolas ,  GIROD, Pierre Alain ,  CALABRESE, David ,  REGAMEY, Alexandre ,  DONINELLI-AROPE, Saline

Inventor： MERMOD, Nicolas ,  GIROD, Pierre Alain ,  CALABRESE, David ,  REGAMEY, Alexandre ,  DONINELLI-AROPE, Saline

IPC: C12N15/09 ,  C12N15/67 ,  C12N15/63 ,  C12Q1/68

CPC classification number: C12N15/63

Abstract: Isolated and purified MAR sequences of human and non-human animal origin are disclosed as are nucleotide sequences corresponding to or based on them. In particular, MARs and MAR constructs with high transcription and/or protein production enhancing activities are disclosed and so are methods for identifying such MARs, designing such MAR constructs and employing them, e.g., for high yield production of proteins.

Abstract translation: 公开了人和非人动物来源的分离和纯化的MAR序列以及对应于或基于它们的核苷酸序列。 具体而言，公开了具有高转录和/或蛋白质产生增强活性的MAR和MAR构建体，以及用于鉴定这种MAR的方法，设计这种MAR构建体并使用它们，例如用于高产量生产蛋白质的方法。

公开(公告)号：WO2005056581A3

公开(公告)日：2005-10-13

申请号：PCT/IB2004004047

申请日：2004-12-09

Applicant: UNIV LAUSANNE ,  VYROUBALOVA EVA ,  FISCH IGOR ,  MERMOD NICOLAS

Inventor： VYROUBALOVA EVA ,  FISCH IGOR ,  MERMOD NICOLAS

IPC: A61K38/00 ,  C07K7/06 ,  C07K7/08 ,  A61K38/08 ,  A61K38/10

CPC classification number: A61K38/00

Abstract: The present invention relates to a peptide able to specifically bind a chemokine receptor consisting essentially in the sequence NPFYYLSFSP, or LLXXXFFXXX, a part thereof, a combination thereof and/or variants. Furthermore, it relates to a pharmaceutical composition comprising as an active substance a pharmaceutically effective amount of at least one of said peptide.

Abstract translation: 本发明涉及能够特异性结合基本上以序列NPFYYLSFSP或LLXXXFFXXX，其一部分，其组合和/或变体组成的趋化因子受体的肽。 此外，它涉及药物组合物，其包含药学有效量的至少一种所述肽作为活性物质。

公开(公告)号：WO2012035508A4

公开(公告)日：2012-07-19

申请号：PCT/IB2011054035

申请日：2011-09-15

Applicant: SPINOMIX S A ,  RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

Inventor： RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

IPC: C12Q1/04 ,  C12Q1/14 ,  C12Q1/56 ,  G01N33/569 ,  G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N33/56938 ,  G01N2333/75 ,  G01N2333/974

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract translation: 从含有纤维蛋白原的样品中分离靶分子或颗粒的方法包括：（a）将至少部分地将样品中所含的纤维蛋白原转化成纤维蛋白，将所述靶分子或颗粒捕获在纤维蛋白网络中; （b）缩回所述纤维蛋白网络以形成纤维蛋白凝块; （c）将所述纤维蛋白凝块与周围的样品介质分离，

公开(公告)号：WO2012035508A2

公开(公告)日：2012-03-22

申请号：PCT/IB2011/054035

申请日：2011-09-15

Applicant: SPINOMIX S.A. ,  RIDA, Amar ,  MERMOD, Nicolas ,  FRANCOIS, Patrice ,  LAZAREVIC, Vladimir ,  SCHRENZEL, Jacques

Inventor： RIDA, Amar ,  MERMOD, Nicolas ,  FRANCOIS, Patrice ,  LAZAREVIC, Vladimir ,  SCHRENZEL, Jacques

IPC: G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N33/56938 ,  G01N2333/75 ,  G01N2333/974

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract translation: 用于从含有纤维蛋白原的样品中分离目标分子或颗粒的方法包括：（a）通过将所述样品中所含的纤维蛋白原至少部分转变成纤维蛋白，将所述目标分子或颗粒捕获在纤维蛋白网络中 ; （b）回缩所述纤维蛋白网络以形成纤维蛋白凝块; （c）将所述纤维蛋白凝块与周围的样品介质分开，

公开(公告)号：WO2011033375A3

公开(公告)日：2011-05-12

申请号：PCT/IB2010002337

申请日：2010-09-20

Applicant: SELEXIS SA ,  MERMOD NICOLAS ,  GIROD PIERRE-ALAIN ,  GRANDJEAN MELANIE ,  LE FOURN VALERIE ,  CALABRESE DAVID ,  REGAMEY ALEXANDRE

Inventor： MERMOD NICOLAS ,  GIROD PIERRE-ALAIN ,  GRANDJEAN MELANIE ,  LE FOURN VALERIE ,  CALABRESE DAVID ,  REGAMEY ALEXANDRE

IPC: C12N15/90 ,  C12N15/113

CPC classification number: C12N15/907 ,  C12N15/85 ,  C12N2830/46

Abstract: Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhanced a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric strucutre which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particualr a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.

Abstract translation: 公开了用于转基因表达的方法和真核宿主细胞。 可以对细胞进行治疗和/或修饰以增加同源重组（HR），减少非同源末端连接（NHEJ）和/或增强所述细胞中的HR / NHEJ比例。 可以用包含转基因的载体转染这样的细胞，其有利地整合到细胞的基因组中以形成可以包含多于200个转基因拷贝的连接结构。 有利地提供某些表达增强元件如MARs以进一步增强和/或促进转基因表达。 还公开了重组真核宿主细胞，特别是非灵长类动物宿主细胞，其包含编码蛋白质和/或RNA的转基因序列，特别是灵长类动物蛋白质和/或RNA，涉及穿过ER膜的易位和/ 或分泌跨细胞质膜。

公开(公告)号：WO2005019253A1

公开(公告)日：2005-03-03

申请号：PCT/CH2004/000536

申请日：2004-08-23

Applicant: OPTIMA ENVIRONNEMENT S.A. ,  MERMOD, Nicolas ,  SUAREZ, Mougli

Inventor： MERMOD, Nicolas ,  SUAREZ, Mougli

IPC: C07K14/415

CPC classification number: A01N37/46 ,  A01N65/00 ,  A61K38/00 ,  C07K14/415 ,  A01N2300/00

Abstract: Protein family derived from the protein defined by the sequence QGPGRQPDFQRCGQQLRNISPPQRCPSLRQAVQLTHQQQGQVGPQQVRQMYRVASNIPST

Abstract translation: 衍生自QGPGRQPDFQRCGQQLRNISPPQRCPSLRQAVQLTHQQQGQVGPQQVRQMYRVASNIPST所定义的蛋白质的蛋白质家族

公开(公告)号：WO2012035508A3

公开(公告)日：2012-05-18

申请号：PCT/IB2011054035

申请日：2011-09-15

Applicant: SPINOMIX S A ,  RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

Inventor： RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

IPC: C12Q1/04 ,  C12Q1/14 ,  C12Q1/56 ,  G01N33/569 ,  G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N33/56938 ,  G01N2333/75 ,  G01N2333/974

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract translation: 从含有纤维蛋白原的样品中分离目标分子或颗粒的方法包括：（a）通过将样品中所含的纤维蛋白原至少部分转化为纤维蛋白，将所述目标分子或颗粒捕获在纤维蛋白网络中; （b）回缩所述纤维蛋白网络以形成纤维蛋白凝块; （c）将所述纤维蛋白凝块与周围的样品介质分开，

公开(公告)号：WO2011033375A2

公开(公告)日：2011-03-24

申请号：PCT/IB2010/002337

申请日：2010-09-20

Applicant: SELEXIS S.A. ,  MERMOD, Nicolas ,  GIROD, Pierre-Alain ,  GRANDJEAN, Mélanie ,  LE FOURN, Valérie ,  CALABRESE, David ,  REGAMEY, Alexandre

Inventor： MERMOD, Nicolas ,  GIROD, Pierre-Alain ,  GRANDJEAN, Mélanie ,  LE FOURN, Valérie ,  CALABRESE, David ,  REGAMEY, Alexandre

IPC: C12N15/90 ,  C12N15/113

CPC classification number: C12N15/907 ,  C12N15/85 ,  C12N2830/46

Abstract: Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhanced a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric strucutre which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particualr a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.

Abstract translation: 公开了用于转基因表达的方法和真核宿主细胞。 可以处理和/或修饰细胞以增加所述细胞中的同源重组（HR），减少非同源末端连接（NHEJ）和/或增强HR / NHEJ比率。 这些细胞可以用包含转基因的载体转染，其有利地整合到细胞的基因组中以形成可包含超过200个转基因拷贝的串联结构。 有利地提供某些表达增强元件如MARs以进一步增强和/或促进转基因表达。 还公开了重组真核宿主细胞，特别是非灵长类宿主细胞，其包含编码蛋白和/或RNA（特别是灵长类蛋白和/或RNA）的转基因序列，所述蛋白和/或RNA涉及穿过ER膜和/ 或通过细胞质膜分泌。

公开(公告)号：WO03008441A2

公开(公告)日：2003-01-30

申请号：PCT/CH0200401

申请日：2002-07-19

Applicant: OPTIMA ENVIRONNEMENT S A ,  MERMOD NICOLAS ,  MARISON IAN WILLIAM ,  DOERRIES STEPHAN CHRISTOS ,  MEYER EVELYNE ,  SUAREZ MOUGLI

Inventor： MERMOD NICOLAS ,  MARISON IAN WILLIAM ,  DOERRIES STEPHAN CHRISTOS ,  MEYER EVELYNE

IPC: C12N15/09 ,  A61K38/00 ,  A61P31/04 ,  B01D21/01 ,  C07K14/415 ,  C12P21/02

CPC classification number: C07K14/415 ,  A61K38/00

Abstract: The invention concerns a new family of proteins obtained from Moringa seeds or derived from Moringa seed proteins. Those proteins can be used for different purposes such as coagulation agents for the water treatment and/or as antibiotic agents.This new protein family consists of at least 5 sub-families :A first being obtained according to a recombinant process. All other sub-families being obtained according to specific extraction processes. Proteins obtained according to the extraction process of the invention will be referred as E proteins in the present text.

Abstract translation: 本发明涉及从辣木种子获得或源自辣木种子蛋白质的新蛋白质家族。 这些蛋白质可以用于不同的目的，例如用于水处理的凝结剂和/或作为抗生素剂。这种新的蛋白质家族由至少5个亚家族组成：首先根据重组方法获得。 所有其他亚科都是根据特定的提取过程获得的。 根据本发明的提取方法获得的蛋白质在本文中将被称为E蛋白质。

公开(公告)号：WO2002074969A3

公开(公告)日：2002-09-26

申请号：PCT/IB2002/002137

申请日：2002-01-28

Applicant: UNIVERSITY OF LAUSANNE ,  MERMOD, Nicolas ,  ZAHN-ZABAL, Monique ,  IMHOF, Markus ,  CHATELLARD, Philippe ,  GIROD, Pierre-Alain

Inventor： MERMOD, Nicolas ,  ZAHN-ZABAL, Monique ,  IMHOF, Markus ,  CHATELLARD, Philippe ,  GIROD, Pierre-Alain

IPC: C12N15/79

Abstract: The present invention relates to compositions and method for transfecting eukaryotic cells with nucleic acid vectors. In particular, the invention relates to uses of MAR elements to increase stable and transient transfection efficiency.