公开(公告)号：WO2006003018A3

公开(公告)日：2006-05-04

申请号：PCT/EP2005007361

申请日：2005-07-07

Applicant: ICON GENETICS AG ,  MARILLONNET SYLVESTRE ,  ENGLER CAROLA ,  MUEHLBAUER STEFAN ,  HERZ STEFAN ,  WERNER STEFAN ,  KLIMYUK VICTOR ,  GLEBA YURI

Inventor： MARILLONNET SYLVESTRE ,  ENGLER CAROLA ,  MUEHLBAUER STEFAN ,  HERZ STEFAN ,  WERNER STEFAN ,  KLIMYUK VICTOR ,  GLEBA YURI

IPC: C12N15/82 ,  A01H5/12 ,  C12N15/74

CPC classification number: C12N15/8203 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8257

Abstract: A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterelogous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.

Abstract translation: 通过在植物或植物叶中表达来自目的序列的所述感兴趣的蛋白质产生感兴趣的蛋白质的方法，包括：a）通过用农杆菌渗入所述植物或所述植物叶片来转染所述植物或所述植物叶子 所述农杆菌菌株在T-DNA中含有具有编码复制子的序列部分的异源DNA序列，其中所述编码复制子的序列含有所述复制子复制子功能所必需的序列，所述序列源自 植物病毒和所述目的序列从所述复制子表达，b）任选地从步骤（a）中渗入的所述植物或所述植物叶中分离所述感兴趣的蛋白质，其中所述土壤杆菌菌株具有第一遗传修饰呈递 所述农杆菌菌株在不存在所述补体因子的情况下用所述T-DNA转染生物体是有缺陷的。

公开(公告)号：WO2006003018A2

公开(公告)日：2006-01-12

申请号：PCT/EP2005/007361

申请日：2005-07-07

Applicant: ICON GENETICS AG ,  MARILLONNET, Sylvestre ,  ENGLER, Carola ,  MÜHLBAUER, Stefan ,  HERZ, Stefan ,  WERNER, Stefan ,  KLIMYUK, Victor ,  GLEBA, Yuri

Inventor： MARILLONNET, Sylvestre ,  ENGLER, Carola ,  MÜHLBAUER, Stefan ,  HERZ, Stefan ,  WERNER, Stefan ,  KLIMYUK, Victor ,  GLEBA, Yuri

IPC: C12N15/82 ,  A01H5/12 ,  C12N15/74

CPC classification number: C12N15/8203 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8257

Abstract: A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterelogous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.

Abstract translation: 通过从植物或植物叶中感兴趣的序列表达所述感兴趣的蛋白质来产生感兴趣的蛋白质的方法，包括：a）通过用农杆菌浸润所述植物或所述植物叶来转染所述植物或所述植物叶子 所述含有T-DNA的所述农杆菌菌株具有编码复制子的序列部分的恒耳DNA序列，其中所述编码复制子的序列含有所述复制子的复制子功能所需的序列，所述序列衍生自 所述复制子表达的所述感兴趣的序列，b）任选地从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述目的蛋白质，其中所述农杆菌菌株被提供第一遗传修饰呈现 所述农杆菌菌株在没有所述补体因子的情况下缺陷用于用所述T-DNA转染生物体。

公开(公告)号：WO2009000432A1

公开(公告)日：2008-12-31

申请号：PCT/EP2008/004775

申请日：2008-06-13

Applicant: ICON GENETICS GMBH ,  STETTNER, Cornelia ,  HERZ, Stefan ,  GOLDS, Timothy

Inventor： STETTNER, Cornelia ,  HERZ, Stefan ,  GOLDS, Timothy

IPC: C12N15/67 ,  C12N15/82 ,  A01H5/00

CPC classification number: C12N15/67 ,  C12N15/8214 ,  C12N15/8216

Abstract: A process of expressing a coding sequence of interest in plastids of a plant, plant tissue or plant cells, said process comprising providing a plant, plant tissue or plant cells with a nucleic acid comprising a translation control element and, downstream thereof, a coding sequence to be expressed in plastids of said plant, plant tissue or plant cells, said translation control element comprising: i) a sequence of SEQ ID NO: 1; or ii) a sequence of SEQ ID NO: 2; or iii) a sequence having a sequence identity of at least 75 % to the sequence of SEQ ID NO: 2.

Abstract translation: 一种在植物，植物组织或植物细胞的质体中表达感兴趣的编码序列的方法，所述方法包括向植物，植物组织或植物细胞提供包含翻译控制元件的核酸，并且在其下游提供编码序列 在所述植物，植物组织或植物细胞的质体中表达，所述翻译控制元件包括：i）SEQ ID NO：1的序列; 或ii）SEQ ID NO：2的序列; 或iii）与SEQ ID NO：2的序列具有至少75％的序列同一性的序列。

公开(公告)号：WO02057466A3

公开(公告)日：2003-02-27

申请号：PCT/EP0200481

申请日：2002-01-18

Applicant: ICON GENETICS AG ,  EIBL CHRISTIAN ,  HUANG FONG-CHIN ,  KLAUS SEBASTIAN ,  MUEHLBAUER STEFAN ,  HERZ STEFAN ,  KOOP HANS-ULRICH

Inventor： EIBL CHRISTIAN ,  HUANG FONG-CHIN ,  KLAUS SEBASTIAN ,  MUEHLBAUER STEFAN ,  HERZ STEFAN ,  KOOP HANS-ULRICH

IPC: A01H5/00 ,  C12N15/09 ,  C12N15/82

CPC classification number: C12N15/8214 ,  C12N15/8209

Abstract: A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.

Abstract translation: 提供了通过以下步骤生产多细胞植物，植物器官或其植物组织转化的方法：（a）改变或破坏质体基因组中的基因的功能以产生可选择或可识别的表型; （b）分离或选择具有表达所述表型的质体的植物或细胞; （c）用具有能够恢复所述功能的恢复序列的至少一个转化载体转化所述分离或选择的植物，植物器官或植物组织的所述质体基因组; 和（d）分离或选择具有表达所述恢复功能的质体的所述转化植物，植物器官或植物组织。

公开(公告)号：WO2004015115A1

公开(公告)日：2004-02-19

申请号：PCT/EP2003/008549

申请日：2003-08-01

Applicant: ICON GENETICS AG ,  HERZ, Stefan ,  KOOP, Hans-Ulrich ,  GOLDS, Timothy, J. ,  EIBL, Christian

Inventor： HERZ, Stefan ,  KOOP, Hans-Ulrich ,  GOLDS, Timothy, J. ,  EIBL, Christian

IPC: C12N15/82

CPC classification number: C12N15/8214

Abstract: A process of generating transgenic plants or plant cells transformed on their plastome, comprising (a) introducing into plant plastids a first DNA molecule and a second DNA molecule, wherein said first DNA molecule contains a first region homologous to a region of the plastome for directing plastome integration and a first sequence of interest, and said second DNA molecule contains a second region homologous to a region of the plastome for directing plastome integration and a second sequence of interest, whereby a sequence segment of said first sequence of interest is homologous to a sequence segment of said second sequence of interest, and selecting transformants having an integration sequence stably integrated in the plastome, whereby said integration sequence contains at least a portion of said first and at least a portion of said second sequence of interest as a continuous sequence.

Abstract translation: 一种产生在其塑料体上转化的转基因植物或植物细胞的方法，包括（a）向植物质体中引入第一DNA分子和第二DNA分子，其中所述第一DNA分子包含与所述塑性体的区域同源的第一区域，用于引导 plastome整合和第一个兴趣序列，并且所述第二DNA分子含有与所述plastome区域同源的第二区域，用于引导Plastome整合和第二感兴趣的序列，其中所述第一感兴趣序列的序列片段与 所述第二感兴趣序列的序列片段，以及选择具有稳定整合在所述塑性体中的整合序列的转化体，由此所述整合序列包含所述第一和至少一部分所述第二感兴趣序列的连续序列的至少一部分。

公开(公告)号：WO02055651A3

公开(公告)日：2003-02-20

申请号：PCT/EP0200301

申请日：2002-01-14

Applicant: ICON GENETICS AG ,  HERZ STEFAN ,  KLAUS SEBASTIAN ,  EIBL CHRISTIAN ,  MUEHLBAUER STEFAN ,  KOOP HANS-ULRICH ,  GLEBA YURI

Inventor： HERZ STEFAN ,  KLAUS SEBASTIAN ,  EIBL CHRISTIAN ,  MUEHLBAUER STEFAN ,  KOOP HANS-ULRICH ,  GLEBA YURI

IPC: A01H5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82

CPC classification number: C12N15/8209 ,  C12N15/8214

Abstract: This invention discloses a novel process and vector therefore for producing a multicellular plant or plant cells having stably transformed plastids. This process comprises transforming plastids or plant cells via homologous recombination with a DNA molecule enabling DNA modification, whereby said DNA molecule comprises a fragment of a gene requiring for expression a sequence element of the host plastid. The invention also includes the use of novel selection inhibitors for plastic transformation and the possibility of performing multiple rounds of transformation without accumulation of resistance genes.

Abstract translation: 本发明公开了一种用于生产具有稳定转化的质体的多细胞植物或植物细胞的新方法和载体。 该方法包括通过与能够进行DNA修饰的DNA分子的同源重组来转化质体或植物细胞，由此所述DNA分子包含需要表达宿主质体的序列元件的基因的片段。 本发明还包括使用新颖的选择抑制剂进行塑性转化和进行多轮转化而不积累抗性基因的可能性。