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    ROTAVIRUS-LIKE PARTICLE PRODUCTION IN PLANTS
    3.
    发明申请
    ROTAVIRUS-LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
    在植物中的旋风样颗粒生产

    公开(公告)号：WO2013166609A1

    公开(公告)日：2013-11-14

    申请号：PCT/CA2013/050364

    申请日：2013-05-10

    Applicant: MEDICAGO INC. ,  MITSUBISHI TANABE PHARMA CORPORATION

    Inventor： D'AOUST, Marc-Andre ,  LANDRY, Nathalie ,  LAVOIE, Pierre-Olivier ,  ARAI, Masaaki ,  ASAHARA, Naomi ,  MUTEPFA, David Levi Rutendo ,  HITZEROTH, Inga Isabel ,  RYBICKI, Edward Peter

    IPC: C12N7/04 ,  A61K39/15 ,  A61P31/14 ,  A61P37/04 ,  C07K14/14 ,  C12N15/46 ,  C12N15/82 ,  C12N7/01

    CPC classification number: A61K39/15 ,  A61K39/12 ,  A61K2039/5258 ,  C07K14/005 ,  C12N7/00 ,  C12N7/02 ,  C12N15/8203 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8221 ,  C12N15/8258 ,  C12N2720/12334 ,  C12N2720/12351

    Abstract: A method of producing a virus-like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant, or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein for example but not limited to rotavirus protein VP2. The nucleotide sequence may further comprise one or more than one amplification element and/or a compartment targeting sequence. A second nucleic acid might be introduced into the plant, or portion of the plant. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP6. Optionally, a third nucleic acid and/ or fourth nucleic acid might be introduced into the plant, or portion of the plant. The third nucleic acid comprises a third regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP4. The fourth nucleic acid comprises a fourth regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP7. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.

    Abstract translation: 提供了一种在植物中生产病毒样颗粒（VLP）的方法。 该方法包括将第一核酸引入植物或植物的一部分。 所述第一核酸包含在植物中活性的第一调节区，其可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP2）的核苷酸序列。 核苷酸序列还可以包含一个或多于一个扩增元件和/或隔室靶向序列。 可以将第二种核酸引入植物或植物的一部分。 第二核酸包含在植物中有活性的第二调控区，并可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP6）的核苷酸序列。 任选地，第三核酸和/或第四核酸可以被引入植物或植物的一部分。 第三核酸包含在植物中活性的第三调节区，并且与编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP4）的核苷酸序列可操作地连接。 第四核酸包含在植物中活性的第四调节区，并且可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP7）的核苷酸序列。 将植物或植物的一部分在允许核酸表达的条件下温育，从而产生VLP。

    PEPTIDES FOR THE BINDING OF NUCLEOTIDE TARGETS
    4.
    发明申请
    PEPTIDES FOR THE BINDING OF NUCLEOTIDE TARGETS 审中-公开
    核心目标结合的肽

    公开(公告)号：WO2013155555A1

    公开(公告)日：2013-10-24

    申请号：PCT/AU2013/000387

    申请日：2013-04-16

    Applicant: THE UNIVERSITY OF WESTERN AUSTRALIA ,  THE STATE BOARD OF HIGHER EDUCATION ON BEHALF OF THE UNIVERSITY OF OREGON

    Inventor： BARKAN, Alice ,  SMALL, Ian ,  ROJAS, Margarita ,  BOND, Charles ,  FUJII, Sota ,  CHONG, Yee Seng

    IPC: C07K14/415 ,  C07K19/00 ,  A61K38/16 ,  C12N15/09

    CPC classification number: C07K14/001 ,  A61K38/00 ,  C07K14/415 ,  C07K2319/24 ,  C07K2319/50 ,  C12N15/1062 ,  C12N15/67 ,  C12N15/8214 ,  C12N15/8216

    Abstract: A method of regulating expression of a gene in a cell is described, comprising the step of introducing into the cell a recombinant polypeptide comprising a PPR RNA-binding domain which itself comprises at least a pair of PPR RNA base-binding motifs. The PPR RNA base- binding motifs of the PPR RNA-binding domain are operably capable of binding the target RNA molecule with a target RNA sequence. Recombinant polypeptides comprising at least one PPR RNA-binding domain capable of binding to target RNA sequence are also described, together with fusion proteins comprising the recombinant PPR RNA-binding domains as well as isolated nucleic acids useful in preparing the recombinant polypeptides described. Recombinant vectors; compositions comprising the recombinant polypeptides; isolated nucleic acids; recombinant vectors; host cells comprising same; use of same in the manufacture of a medicament for regulating gene expression; as well as systems and kits for regulating gene expression are also described.

    Abstract translation: 描述了调节细胞中基因表达的方法，其包括将包含本身包含至少一对PPR RNA碱基结合基序的PPR RNA结合结构域的重组多肽引入细胞的步骤。 PPR RNA结合结构域的PPR RNA碱基结合基序可操作地能够将靶RNA分子与靶RNA序列结合。 还描述了包含能够结合靶RNA序列的至少一个PPR RNA结合结构域的重组多肽，以及包含重组PPR RNA结合结构域的融合蛋白以及可用于制备所述重组多肽的分离的核酸。 重组载体; 包含重组多肽的组合物; 分离核酸; 重组载体; 包含其的宿主细胞; 在制备用于调节基因表达的药物中的用途; 还描述了用于调节基因表达的系统和试剂盒。

    LTTR-MODULATED EXPRESSION IN GENTICALLY MODIFIED PHOTOSYNTHETIC ORGANISMS
    6.
    发明申请
    LTTR-MODULATED EXPRESSION IN GENTICALLY MODIFIED PHOTOSYNTHETIC ORGANISMS 审中-公开
    通用改性光合作用机制中的LTTR调制表达

    公开(公告)号：WO2010151854A3

    公开(公告)日：2011-03-31

    申请号：PCT/US2010040122

    申请日：2010-06-27

    Applicant: DONALD DANFORTH PLANT SCI CT ,  SUBRAMANIAN SENTHIL

    Inventor： SUBRAMANIAN SENTHIL

    IPC: A01H13/00 ,  A01H5/00 ,  C12N15/63 ,  C12N15/82

    CPC classification number: C12N15/8214 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8238

    Abstract: This invention provides a method of conveniently regulating transcription of a polynucleotide of interest in a photosynthetic organism by the administration of a modulator. The photosynthetic organism comprises 1) an LTTR trans-acting factor and 2) a promoter functional in the host, an LTTR cis-element, and a polynucleotide of interest, wherein the promoter and the LTTR cis-element are operably linked to the polynucleotide of interest..

    Abstract translation: 本发明提供了通过施用调节剂方便地调节光合生物体中目的多核苷酸转录的方法。 光合生物体包括1）LTTR反式作用因子和2）在宿主中有功能的启动子，LTTR顺式元件和感兴趣的多核苷酸，其中启动子和LTTR顺式元件可操作地连接到 利益..

    IMPROVED PRODUCTION OF PROTEINS WITH DOWNSTREAM BOX FUSIONS IN PLASTIDS AND IN BACTERIA
    10.
    发明申请
    IMPROVED PRODUCTION OF PROTEINS WITH DOWNSTREAM BOX FUSIONS IN PLASTIDS AND IN BACTERIA 审中-公开
    在水稻和细菌中改进下游蛋白质融合蛋白的生产

    公开(公告)号：WO2010021961A2

    公开(公告)日：2010-02-25

    申请号：PCT/US2009/053981

    申请日：2009-08-17

    Applicant: CORNELL UNIVERSITY ,  HANSON, Maureen, R. ,  GRAY, Benjamin, N. ,  AHNER, Beth A.

    Inventor： HANSON, Maureen, R. ,  GRAY, Benjamin, N. ,  AHNER, Beth A.

    IPC: C12N15/11 ,  C07K7/08 ,  C12N15/63 ,  A01H1/00

    CPC classification number: C12N9/2434 ,  C07K7/08 ,  C07K14/55 ,  C12N15/67 ,  C12N15/8214 ,  C12N15/8257

    Abstract: The present invention is directed to the use of certain selected downstream box ("DB") regions and codon-optimized DB regions to achieve high-level protein expression in transformed organisms or organelles. In particular, high level protein expression in plastids and bacteria can be achieved by fusion of the TetC or NPTII DB region to a gene of interest. Protein expression in a transformed organism or organelle can also be enhanced by optimization of codon usage within the DB region based on codons preferentially found in the DB regions of highly expressed native genes in the organism or organelle. Methods for enhanced protein expression, and related nucleic acid molecules, expression vectors, transformed cells, and transgenic organisms, are provided. The present invention is particularly useful for expressing cellulolytic enzymes, including a suite of several cellulolytic enzymes, in plastids, bacteria and algae.

    Abstract translation: 本发明涉及某些选择的下游盒（“DB”）区域和密码子优化的DB区域在转化的生物体或细胞器中实现高水平蛋白质表达的用途。 特别地，质体和细菌中的高水平蛋白质表达可以通过将TetC或NPTII DB区域融合到感兴趣的基因来实现。 转化的生物或细胞器中的蛋白质表达也可以通过基于在生物体或细胞器中高度表达的天然基因的DB区域中优先存在的密码子优化DB区域内的密码子来增强。 提供了增强蛋白质表达的方法，以及相关核酸分子，表达载体，转化细胞和转基因生物。 本发明特别可用于在质体，细菌和藻类中表达含有几种纤维素分解酶的纤维素分解酶。

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