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    GENETICALLY ENCODED FRET-BASED MMP-9 ACTIVITY BIOSENSOR AND USE THEREOF
    1.
    发明申请
    GENETICALLY ENCODED FRET-BASED MMP-9 ACTIVITY BIOSENSOR AND USE THEREOF 审中-公开
    基因编码基于MMP-9的活性生物传感器及其用途

    公开(公告)号：WO2015022646A2

    公开(公告)日：2015-02-19

    申请号：PCT/IB2014/063883

    申请日：2014-08-12

    申请人： INSTYTUT BIOLOGII DOSWIADCZALNEJ IM. MARCELEGO NENCKIEGO POLSKA AKADEMIA NAUK

    发明人： STAWARSKI, Michal ,  WLODARCZYK, Jakub ,  KACZMAREK, Leszek

    IPC分类号： G01N33/542

    CPC分类号： C12Q1/37 ,  C07K14/00 ,  C07K2319/50 ,  C07K2319/60 ,  G01N33/542 ,  G01N2333/96494

    摘要： The present invention relates to a genetically encoded FRET-based biosensor to monitor the activity of matrix metalloproteinase 9 (MMP-9). MMP-9 is an extracellular acting endopeptidase implicated in both physiological and pathological processes. A genetically encoded FRET biosensor anchored in the cellular membrane allows studying the proteolytic activity of MMP-9 with high spatiotemporal resolution at the exact region of MMP-9 action on the cell. Applicability of the biosensor, both in vitro and in vivo in living cells, has been demonstrated by ratiometric analysis of cleavage of the biosensor by a purified auto-activating mutant of MMP-9.

    摘要翻译： 本发明涉及基因编码的基于FRET的生物传感器，用于监测基质金属蛋白酶9（MMP-9）的活性。 MMP-9是涉及生理和病理过程的胞外作用内肽酶。 锚定在细胞膜中的遗传编码的FRET生物传感器允许在细胞的MMP-9作用的确切区域以高时空分辨率研究MMP-9的蛋白水解活性。 生物传感器在活体细胞中的体外和体内的适用性已经通过MMP-9纯化的自动激活突变体对生物传感器的切割进行比较分析来证明。

    USE OF CACYBP/SIP IN THE TREATMENT AND DIAGNOSIS OF ALZHEIMER'S DISEASE
    4.
    发明申请
    USE OF CACYBP/SIP IN THE TREATMENT AND DIAGNOSIS OF ALZHEIMER'S DISEASE 审中-公开
    CACYBP / SIP在阿尔茨海默病的治疗和诊断中的应用

    公开(公告)号：WO2013171233A1

    公开(公告)日：2013-11-21

    申请号：PCT/EP2013/059969

    申请日：2013-05-14

    申请人： INSTYTUT BIOLOGII DOSWIADCZALNEJ IM. MARCELEGO NENCKIEGO POLSKA AKADEMIA NAUK

    发明人： FILIPEK, Anna ,  NIEWIADOMSKA, Grazyna ,  SCHNEIDER, Gabriela ,  WASIK, Urszula

    IPC分类号： A61K38/17 ,  A61P25/28

    CPC分类号： A61K38/1709

    摘要： CacyBP/SIP interacts with several targets, including tubulin, and was shown to play a role in the organization of microtubules. To better understand the involvement of CacyBP/SIP in cytoskeletal physiology and pathophysiology, the neuronal redistribution and possible function of CacyBP/SIP in Alzheimer disease (AD) pathology were investigated. CacyBP/SIP exhibits phosphatase activity towards phosphorylated tau, which suggests it might play a role in AD pathology specifically involving tau protein. Therefore, CacyBP/SIP may be useful in the diagnosis and treatment of AD.

    摘要翻译： CacyBP / SIP与几种靶标（包括微管蛋白）相互作用，并被证明在微管的组织中发挥作用。 为了更好地了解CacyBP / SIP在细胞骨架生理学和病理生理学中的参与，研究了CacyBP / SIP在阿尔茨海默病（AD）病理学中的神经元再分配和可能的功能。 CacyBP / SIP对磷酸化tau表现出磷酸酶活性，这表明它可能在特异性涉及tau蛋白的AD病理学中起作用。 因此，CacyBP / SIP可能有助于AD的诊断和治疗。