公开(公告)号：WO2005075653A1

公开(公告)日：2005-08-18

申请号：PCT/KR2005/000356

申请日：2005-02-04

Applicant: BIOLEADERS CORPORATION ,  M.D. LAB Co. Ltd ,  BIOLEADERS JAPAN CORPORATION ,  IMBIO CORPORATION ,  SUNG, Moon Hee ,  KIM, Chul Joong ,  JUNG, Chang Min ,  HONG, Seung Pyo ,  LEE, Jong Su ,  CHOI, Jae Chul ,  SEWAKI, Motomitsu ,  KIM, Jong-Taik

Inventor： SUNG, Moon Hee ,  KIM, Chul Joong ,  JUNG, Chang Min ,  HONG, Seung Pyo ,  LEE, Jong Su ,  CHOI, Jae Chul ,  SEWAKI, Motomitsu ,  KIM, Jong-Taik

IPC: C12N15/63

CPC classification number: C07K14/005 ,  C12N15/74 ,  C12N2750/14322

Abstract: The present invention relates to a surface expression vector expressing a parvovirus antigen on the surface of microorganisms, the vector containing not only a gene encoding the capsid antigen protein of parvovirus causing canine parvovirus (CPV) infection and feline panleukopenia (FLP) but also at least one of pgsB, pgsC and pgsA genes encoding a poly-gamma-glutamate synthetase complex. Also, the present invention relates to microorganisms transformed with the surface expression vector, and parvovirus vaccines containing the transformed microorganisms. According to the present invention, the use of the recombinant bacterial strains expressing the parvovirus antigen on their surface allows the economical production of vaccines for the treatment and prevention of canine parvovirus infection and feline panleukopenia.

Abstract translation: 本发明涉及表达微生物表面的细小病毒抗原的表达表达载体，该载体不仅含有编码引起犬细小病毒（CPV）感染和猫泛白细胞减少症（FLP）的细小病毒的衣壳抗原蛋白的基因，而且至少 编码聚-γ-谷氨酸合成酶复合物的pgsB，pgsC和pgsA基因之一。 此外，本发明涉及用表面表达载体转化的微生物和含有转化的微生物的细小病毒疫苗。 根据本发明，在其表面上使用表达细小病毒抗原的重组细菌菌株可以经济地生产用于治疗和预防犬细小病毒感染和猫泛白细胞减少症的疫苗。

公开(公告)号：WO2004108937A1

公开(公告)日：2004-12-16

申请号：PCT/KR2004/001341

申请日：2004-06-04

Applicant: BIOLEADERS CORPORATION ,  M.D. LAB ,  BIOLEADERS JAPAN CORP. ,  KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY ,  SUNG, Moon Hee ,  KIM, Chul Joong ,  JUNG, Chang Min ,  HONG, Seung Pyo ,  LEE, Jong Su ,  CHOI, Jae Chul ,  KIM, Kwang ,  SHUNICHI, Kuroda ,  POO, Ha Ryoung

Inventor： SUNG, Moon Hee ,  KIM, Chul Joong ,  JUNG, Chang Min ,  HONG, Seung Pyo ,  LEE, Jong Su ,  CHOI, Jae Chul ,  KIM, Kwang ,  SHUNICHI, Kuroda ,  POO, Ha Ryoung

IPC: C12N15/86

CPC classification number: C07K14/005 ,  A61K39/00 ,  A61K2039/523 ,  C07K2319/00 ,  C12N9/93 ,  C12N15/74 ,  C12N2710/20022 ,  C12N2770/20022 ,  Y02A50/472 ,  Y02A50/476

Abstract: The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

Abstract translation: 本发明涉及SARS冠状病毒抗原的表达表达载体，其含有编码SARS诱导冠状病毒抗原的基因和编码聚-γ-谷氨酸合酶复合物的pgsB，pgsC和pgsA基因中的一种或两种以上的基因，微生物转化 通过表面表达载体和包含该微生物的SARS疫苗。 根据本发明，可以在表面上使用表达SARS冠状病毒抗原的重组菌株经济地制备用于预防和治疗SARS的疫苗。

公开(公告)号：WO2004035795A1

公开(公告)日：2004-04-29

申请号：PCT/KR2003/002163

申请日：2003-10-17

Applicant: BIOLEADERS CORPORATION ,  KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY ,  SUNG, Moon-Hee ,  POO, Ha-Ryoung ,  LEE, Jong-Soo ,  JUNG, Chang-Min ,  HONG, Seung-Pyo ,  KIM, Chul-Joong ,  PARK, Sue-nie ,  PYO, Hyun-mi

Inventor： SUNG, Moon-Hee ,  POO, Ha-Ryoung ,  LEE, Jong-Soo ,  JUNG, Chang-Min ,  HONG, Seung-Pyo ,  KIM, Chul-Joong ,  PARK, Sue-nie ,  PYO, Hyun-mi

IPC: C12N15/63

CPC classification number: C07K14/005 ,  A61K39/0011 ,  A61K2039/523 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2730/10122 ,  C12N2830/008

Abstract: The current invention relates to expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface and bacterial strains harboring such surface display vectors and the use of the bacterial strains or it's extract, purified products as complex vaccines. More particularly, the present invention relates to surface display vectors contains one or more than two genes selected among pgsB, pgsC and pgsA, encoding a poly- -glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. Strain and genes which encode virion capsid proteins, tumor-associated proteins of human papillomavirus, and method for preparing the same.

Abstract translation: 本发明涉及可以有效地产生病毒体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白在微生物表面上的表达载体和含有这种表面显示载体的细菌菌株以及使用细菌菌株或其提取物，纯化产物作为复合疫苗 。 更具体地，本发明涉及含有一个或多于两个选自pgsB，pgsC和pgsA的基因的表面显示载体，其编码芽孢杆菌属的多聚谷氨酸合成酶复合物（pgsBCA）。 编码病毒载体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白的菌株和基因及其制备方法。

公开(公告)号：WO2017132306A1

公开(公告)日：2017-08-03

申请号：PCT/US2017/015015

申请日：2017-01-26

Applicant: RHODIA OPERATIONS ,  JUNG, Chang Min ,  SHEN, Lingjuan ,  ZHOU, Jian ,  QU, Qi

Inventor： JUNG, Chang Min ,  SHEN, Lingjuan ,  ZHOU, Jian ,  QU, Qi

IPC: C09K8/18 ,  C09K8/86

CPC classification number: C09K8/035 ,  C09K8/42 ,  C09K8/528 ,  C09K8/602 ,  C09K8/605 ,  C09K8/68 ,  C09K8/74 ,  C09K8/80 ,  C09K2208/12 ,  C09K2208/24 ,  C09K2208/26 ,  C09K2208/32 ,  C09K2208/34 ,  E21B21/00

Abstract: Methods of using a clay stabilizing agent to inhibit swelling and/or migration of clay subterranean materials upon exposure to water, wherein the clay stabilizing agent is selected from quaternary ammonium salts according to Formula I, Formula II, Formula III, Formula IV, Formula V, and combinations thereof.

Abstract translation: 使用粘土稳定剂抑制粘土地下材料暴露于水时膨胀和/或迁移的方法，其中粘土稳定剂选自根据式I，式II的季铵盐， 式III，式IV，式V及其组合。

公开(公告)号：WO2003014360A1

公开(公告)日：2003-02-20

申请号：PCT/KR2002/001522

申请日：2002-08-09

Applicant: BIOLEADERS CORPORATION ,  M. D. LAB CO., LTD. ,  SUNG, Moon-Hee ,  HONG, Seung-Pyo ,  LEE, Jong-Su ,  JUNG, Chang-Min ,  KIM, Chul-Joong ,  SODA, Kenji ,  ASHIUCHI, Makoto

Inventor： SUNG, Moon-Hee ,  HONG, Seung-Pyo ,  LEE, Jong-Su ,  JUNG, Chang-Min ,  KIM, Chul-Joong ,  SODA, Kenji ,  ASHIUCHI, Makoto

IPC: C12N15/63

CPC classification number: C12N9/1051 ,  C07K2319/02 ,  C12N9/1288 ,  C12Y204/01133 ,  C12Y207/08005

Abstract: The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

Abstract translation: 本发明涉及具有pgsBCA，编码聚-γ-谷氨酸合成酶的基因的表达表达载体和使用载体在微生物表面表达靶蛋白的方法。 插入外来基因的载体转化微生物，使外源蛋白稳定表达在微生物表面。